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The map label for this gene is lpdA [H]

Identifier: 238028029

GI number: 238028029

Start: 2812952

End: 2814721

Strand: Reverse

Name: lpdA [H]

Synonym: bglu_1g24790

Alternate gene names: 238028029

Gene position: 2814721-2812952 (Counterclockwise)

Preceding gene: 238028030

Following gene: 238028027

Centisome position: 72.05

GC content: 65.99

Gene sequence:

>1770_bases
ATGAGTCTCATCGAAGTCAAAGTGCCGGACATCGGCGATTACAACGATATTCCCGTCATCGAGGTGCTGGTGAAGGCGGG
CGACACGGTCGAGAAGGAGCAATCGCTCGTCACGCTCGAATCGGACAAGGCGACGATGGACGTGCCGAGTTCGGCGGCCG
GCGTCGTCAAGGAAGTGAAGGTCAAGGTCGGTGATCCGGTCTCGCAGGGCACGGTGATCGTCGTGCTCGAGGGCGCCGCC
GAGGCGGCCCAGCCCGCTGCAAAGGCGCCCGAGGCGTCGGCCGCCAAGGCCGCCGAGAAGCCGGCTGAAAAACCCGCCGA
GAAGGCCGCGCCGCAGGCCGGCAGCTACTCGGGCAAGGCCGACGTCGAATGCGACATGCTGGTGCTCGGCTCCGGCCCCG
GCGGCTACTCGGCGGCGTTCCGCTCGGCCGATCTCGGCATGAAGACGGTGCTCGTCGAGCGTTACGCGACGCTCGGCGGC
GTGTGCCTGAACGTCGGCTGCATCCCGTCGAAGGCGTTGCTGCACACCGCGCTGGTGATCGACGAGGCGGCTGCGCTGGC
CGATCACGGCATCACGTTCGGCAAGCCCGAGGTCAATCTCGACAAGCTGCGCGACTTCAAGTCGAGCGTGGTCAAGAAGC
TCACGGTCGGCCTCGCCGGCATGGCGAAGGCGCGTAAGGTCCAGGTGGTCTCGGGCGTCGGCAGCTTCGTCGATCCGTAC
CATCTCGAAGTCGAGGGCGAGGGCGGCAAGACCGTCGTCAAGTTCAAGCAGGCGATCATCGCGGCCGGCTCGCAGGCGGT
GAAGCTGCCGTTCATGCCGGAGGACCCGCGCGTGATCGATTCGACCGGCGCGCTCGAATTGCGCCAGCTGCCCAAGCGCA
TGCTCGTCATCGGCGGCGGCATTATCGGCCTCGAAATGGCCACCGTCTACTCGACGCTCGGCGCCGAGATCGACGTGGTC
GAAATGATGGACGGCCTGATGATGGGAGCCGACCGCGATCTCGTGAAGGTCTGGGAGAAGTACAACGCGAAGCGCTTCGG
CAACGTGATGCTGAAGACCAAGACGGTGGGCGCCGAGGCGAAGGAAGACGGCATCTACGTCAAGTTCGAGGGGGAGAAGG
CCCCTGCGGACGCGCAGCGTTACGACCTCGTGCTGGTGGCGGTCGGCCGCAGCCCGAACGGCAAGAAGATCGGTGCCGAC
AAGGCCGGCGTTGCGGTCACGGATCGCGGCTTCATCGAAGTCGACAAGCAGATGCGCACCAATGTGCCGCACATCTTCGC
GATCGGCGACATCGTCGGCCAGCCGATGCTCGCCCACAAGGCCGTGCATGAAGGCCACGTCGCGGCCGAGGCCGCGCACG
GCGAAAAGGCCTATTTCGACGCGCTGCAGATCCCGTCGGTGGCCTACACCGATCCGGAAGTGGCCTGGGCCGGCAAGACC
GAGGACCAACTGAAGGCGGAAGGCGTCAAGTACGGCAAGGCGGTGTTCCCGTGGGCCGCGTCGGGCCGTGCAATCGCCAA
TGGCCGCGACGAGGGCTTCACGAAGCTCCTGTTCGACGAGGAAACGCATCGCGTGATCGGCGGCGGAATCGTCGGCCTGA
ACGCGGGCGACCTGATCAGCGAGGTATGCCTCGCGGTCGAGATGGGCGCCGACGCCGAAGACATCGGCAAGACGATCCAT
CCGCACCCGACGCTCGGCGAATCGATCGGGATGGCCGCCGAGCTGTACGAAGGCGTTTGCACCGACCTGCCGCCGCAGCG
GAAGAAGTAA

Upstream 100 bases:

>100_bases
GCGGCCATCGCGCTGCGCGCGGGAACCGCCTCGGCGGCGCGCGCAGCGGCGCGGATGCGTCGTTCGATCGGCGGTAGTCC
ATACAAGTAGGGGACAGTTC

Downstream 100 bases:

>100_bases
CGGGTGGTCGGGCCGGCGCGCGGGTGGCGCCGGCCCGATGCGATGCGCCGGCCCCGCCGGACCGAGGAAGGTCCGGCGGG
GCCGTTTTCATTGGCGCGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSLIEVKVPDIGDYNDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDPVSQGTVIVVLEGAA
EAAQPAAKAPEASAAKAAEKPAEKPAEKAAPQAGSYSGKADVECDMLVLGSGPGGYSAAFRSADLGMKTVLVERYATLGG
VCLNVGCIPSKALLHTALVIDEAAALADHGITFGKPEVNLDKLRDFKSSVVKKLTVGLAGMAKARKVQVVSGVGSFVDPY
HLEVEGEGGKTVVKFKQAIIAAGSQAVKLPFMPEDPRVIDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVV
EMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPADAQRYDLVLVAVGRSPNGKKIGAD
KAGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
EDQLKAEGVKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIH
PHPTLGESIGMAAELYEGVCTDLPPQRKK

Sequences:

>Translated_589_residues
MSLIEVKVPDIGDYNDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDPVSQGTVIVVLEGAA
EAAQPAAKAPEASAAKAAEKPAEKPAEKAAPQAGSYSGKADVECDMLVLGSGPGGYSAAFRSADLGMKTVLVERYATLGG
VCLNVGCIPSKALLHTALVIDEAAALADHGITFGKPEVNLDKLRDFKSSVVKKLTVGLAGMAKARKVQVVSGVGSFVDPY
HLEVEGEGGKTVVKFKQAIIAAGSQAVKLPFMPEDPRVIDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVV
EMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPADAQRYDLVLVAVGRSPNGKKIGAD
KAGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
EDQLKAEGVKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIH
PHPTLGESIGMAAELYEGVCTDLPPQRKK
>Mature_588_residues
SLIEVKVPDIGDYNDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDPVSQGTVIVVLEGAAE
AAQPAAKAPEASAAKAAEKPAEKPAEKAAPQAGSYSGKADVECDMLVLGSGPGGYSAAFRSADLGMKTVLVERYATLGGV
CLNVGCIPSKALLHTALVIDEAAALADHGITFGKPEVNLDKLRDFKSSVVKKLTVGLAGMAKARKVQVVSGVGSFVDPYH
LEVEGEGGKTVVKFKQAIIAAGSQAVKLPFMPEDPRVIDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVVE
MMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPADAQRYDLVLVAVGRSPNGKKIGADK
AGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
DQLKAEGVKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIHP
HPTLGESIGMAAELYEGVCTDLPPQRKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=41.5384615384615, Blast_Score=338, Evalue=1e-92,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=27.3706896551724, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=27.816091954023, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=26.8131868131868, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI148277071, Length=442, Percent_Identity=24.4343891402715, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI33519430, Length=443, Percent_Identity=24.6049661399549, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519428, Length=443, Percent_Identity=24.6049661399549, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519426, Length=443, Percent_Identity=24.6049661399549, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI148277065, Length=442, Percent_Identity=24.4343891402715, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI291045268, Length=428, Percent_Identity=26.4018691588785, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=65.8174097664543, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=473, Percent_Identity=30.0211416490486, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=25.5555555555556, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.8288288288288, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=58.9041095890411, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=43.0735930735931, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=26.6112266112266, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=25.9259259259259, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=443, Percent_Identity=24.8306997742664, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=458, Percent_Identity=40.3930131004367, Blast_Score=307, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.9317697228145, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=26.4069264069264, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358499, Length=453, Percent_Identity=44.3708609271523, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24640551, Length=515, Percent_Identity=27.5728155339806, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=28.1573498964803, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=27.9166666666667, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=25.625, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61919; Mature: 61788

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIEVKVPDIGDYNDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVK
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHHE
VKVGDPVSQGTVIVVLEGAAEAAQPAAKAPEASAAKAAEKPAEKPAEKAAPQAGSYSGKA
EEECCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCCCCCCCCCC
DVECDMLVLGSGPGGYSAAFRSADLGMKTVLVERYATLGGVCLNVGCIPSKALLHTALVI
CCEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
DEAAALADHGITFGKPEVNLDKLRDFKSSVVKKLTVGLAGMAKARKVQVVSGVGSFVDPY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCE
HLEVEGEGGKTVVKFKQAIIAAGSQAVKLPFMPEDPRVIDSTGALELRQLPKRMLVIGGG
EEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCHHEEEECCC
IIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEA
CHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCC
KEDGIYVKFEGEKAPADAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRT
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHC
NVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
CCCEEEEEHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHHHHHCCCCEEECCCCEEECCCC
EDQLKAEGVKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLIS
HHHHHHCCCCCCCEECCCCCCCCEECCCCCCCCEEEEECCCCCEEECCCEEECCHHHHHH
EVCLAVEMGADAEDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQRKK
HHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLIEVKVPDIGDYNDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVK
CEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHHE
VKVGDPVSQGTVIVVLEGAAEAAQPAAKAPEASAAKAAEKPAEKPAEKAAPQAGSYSGKA
EEECCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCCCCCCCCCC
DVECDMLVLGSGPGGYSAAFRSADLGMKTVLVERYATLGGVCLNVGCIPSKALLHTALVI
CCEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
DEAAALADHGITFGKPEVNLDKLRDFKSSVVKKLTVGLAGMAKARKVQVVSGVGSFVDPY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCE
HLEVEGEGGKTVVKFKQAIIAAGSQAVKLPFMPEDPRVIDSTGALELRQLPKRMLVIGGG
EEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCHHEEEECCC
IIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEA
CHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCC
KEDGIYVKFEGEKAPADAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRT
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHC
NVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
CCCEEEEEHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHHHHHCCCCEEECCCCEEECCCC
EDQLKAEGVKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLIS
HHHHHHCCCCCCCEECCCCCCCCEECCCCCCCCEEEEECCCCCEEECCCEEECCHHHHHH
EVCLAVEMGADAEDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQRKK
HHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]