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The map label for this gene is pdhB [H]

Identifier: 238028030

GI number: 238028030

Start: 2814873

End: 2816507

Strand: Reverse

Name: pdhB [H]

Synonym: bglu_1g24800

Alternate gene names: 238028030

Gene position: 2816507-2814873 (Counterclockwise)

Preceding gene: 238028031

Following gene: 238028029

Centisome position: 72.1

GC content: 67.58

Gene sequence:

>1635_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGATATCGGCGATTACAAGGACATCCCCGTCATCGAGGTGCTGGTGAAGGC
GGGCGATACGGTGGAGGCCGAGCAATCGCTCGTCACGCTCGAGTCCGACAAGGCGACGATGGACGTGCCGAGCCCGTCGG
CCGGCACGGTCAAGGAAGTGAAGGTCAAGGTGGGCGACACCGTGTCGGAAGGCACGCTGATCGTGGTGTTCGAAGGCGCG
GGCGACGCCAAGGCGGAAGCGCCGAAGGCCGAGGCCCCGCAAGCGGCAGCCGCGCCGGCCAAGGCCGAGGCGCCGAAGGC
CGACGCTTTGGCGGGCGGCGGCACGGTCGAGGTCAAGGTGCCGGACATCGGCGACTACAAGGACATTCCCGTCATCGAGA
TCGGCGTGAAGGTCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATGGACGTG
CCGAGCCCGGCGGCCGGCACCGTCAAGGAAATCAAGGTCAAGGTCGGCGACACGGTATCCGAAGGCGCGCTGATCGTGGT
GCTGGAAAGTGGCGACGCCGCGCCGGCTGCGGCTCCGAAGGCGCAAGCGCCGAAGGCCGAGGCGCCGAAGGCGGCGCCGG
CCCCGGCCGCGCAGGCTTCGGCTCCGGCTCCGGCTCCGGCTCCGGCTCCGGCTCCGGCTCCGGCCGCGTCGGGCCGAGCG
AGCCATGCTTCGCCGTCGGTGCGCAAGTTCGCGCGCGAGCTCGGCGTGGACGTCGGCCGCGTGACGGGTTCGGGTCCGAA
GGGCCGCATCACGAAGGAAGACGTGACGGCGTTCGTGAAGGGCGTGATGACGGGCCAGACCGCGGCCCCGGCCGGCGCTG
CCGCGCCGGCGGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAGATCGACTTCACGAAGTTCGGCCCGGTCGAGGCG
CAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACTTGCACCGCAACTGGGTCATGATCCCGCACGTCACCAACAA
CGACGAGGCGGACATCACCGAGCTCGAGGCGCTGCGCGTCCAGCTGAACAAGGAAAACGAGAAGTCGGGCGTGAAGTTCA
CGATGCTCGCCTTCGTCATCAAGGCCGTGGTCGCGGCGCTGAAGAAGTTCCCGACCTTCAACGCGAGCCTGGACGGCGAC
AACCTGATCCTCAAGCAGTACTTCCACATCGGGTTCGCCGCCGATACGCCGAACGGGCTGGTGGTGCCGGTGATCCGCGA
CGCGGACAAGAAGGGCCTCGTCGACATCGCCAAGGAAATGGCCGACCTGTCGAAGGCGGCCCGTGACGGCAAGCTCAAGC
CCGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTGGGCGGCATCGGCGGCACGCATTTCACGCCGATCGTCAAC
GCGCCGGAAGTCGCGATTCTCGGTCTGTCGCGCAGTGCGATGAAGCCGGTCTGGGACGGCAAGCAGTTCGTGCCGCGCCT
AACGCTGCCGATGTCGCTGTCATACGATCACCGCGTGATCGACGGCGCCGCAGCCGCGCGCTTCAACGCTTACCTGGCGT
CGATCCTCGGCGATTTCCGCCGCGTGATTCTTTGA

Upstream 100 bases:

>100_bases
CGATCCGTCGAAACCCAATCCGATGACGGTTTAAACGCATACGTGCCATGTCACGCCGCGCCGCCCGACCAGGGCGGCGC
GGCCCAGGAGACTTTTACAG

Downstream 100 bases:

>100_bases
TGCATGACGGCATCCGCCGTTGGCCCGCGCGGTGTGCCGGATGCGCGCCGCGCGGCCATCGCGCTGCGCGCGGGAACCGC
CTCGGCGGCGCGCGCAGCGG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEAEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDTVSEGTLIVVFEGA
GDAKAEAPKAEAPQAAAAPAKAEAPKADALAGGGTVEVKVPDIGDYKDIPVIEIGVKVGDTVEKEQSLVTLESDKATMDV
PSPAAGTVKEIKVKVGDTVSEGALIVVLESGDAAPAAAPKAQAPKAEAPKAAPAPAAQASAPAPAPAPAPAPAPAASGRA
SHASPSVRKFARELGVDVGRVTGSGPKGRITKEDVTAFVKGVMTGQTAAPAGAAAPAGGGELNLLPWPKIDFTKFGPVEA
QPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKSGVKFTMLAFVIKAVVAALKKFPTFNASLDGD
NLILKQYFHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMADLSKAARDGKLKPDQMQGGCFSISSLGGIGGTHFTPIVN
APEVAILGLSRSAMKPVWDGKQFVPRLTLPMSLSYDHRVIDGAAAARFNAYLASILGDFRRVIL

Sequences:

>Translated_544_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEAEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDTVSEGTLIVVFEGA
GDAKAEAPKAEAPQAAAAPAKAEAPKADALAGGGTVEVKVPDIGDYKDIPVIEIGVKVGDTVEKEQSLVTLESDKATMDV
PSPAAGTVKEIKVKVGDTVSEGALIVVLESGDAAPAAAPKAQAPKAEAPKAAPAPAAQASAPAPAPAPAPAPAPAASGRA
SHASPSVRKFARELGVDVGRVTGSGPKGRITKEDVTAFVKGVMTGQTAAPAGAAAPAGGGELNLLPWPKIDFTKFGPVEA
QPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKSGVKFTMLAFVIKAVVAALKKFPTFNASLDGD
NLILKQYFHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMADLSKAARDGKLKPDQMQGGCFSISSLGGIGGTHFTPIVN
APEVAILGLSRSAMKPVWDGKQFVPRLTLPMSLSYDHRVIDGAAAARFNAYLASILGDFRRVIL
>Mature_543_residues
SQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEAEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDTVSEGTLIVVFEGAG
DAKAEAPKAEAPQAAAAPAKAEAPKADALAGGGTVEVKVPDIGDYKDIPVIEIGVKVGDTVEKEQSLVTLESDKATMDVP
SPAAGTVKEIKVKVGDTVSEGALIVVLESGDAAPAAAPKAQAPKAEAPKAAPAPAAQASAPAPAPAPAPAPAPAASGRAS
HASPSVRKFARELGVDVGRVTGSGPKGRITKEDVTAFVKGVMTGQTAAPAGAAAPAGGGELNLLPWPKIDFTKFGPVEAQ
PLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKSGVKFTMLAFVIKAVVAALKKFPTFNASLDGDN
LILKQYFHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMADLSKAARDGKLKPDQMQGGCFSISSLGGIGGTHFTPIVNA
PEVAILGLSRSAMKPVWDGKQFVPRLTLPMSLSYDHRVIDGAAAARFNAYLASILGDFRRVIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=450, Percent_Identity=30.8888888888889, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI31711992, Length=421, Percent_Identity=30.6413301662708, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=27.4298056155508, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI203098753, Length=428, Percent_Identity=28.0373831775701, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=33.6206896551724, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI1786305, Length=543, Percent_Identity=54.8802946593002, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=30.3240740740741, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=30.0715990453461, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=31.6628701594533, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=36.6666666666667, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=32.5732899022801, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=30.0480769230769, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=435, Percent_Identity=29.4252873563218, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=31.6901408450704, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=31.1688311688312, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=31.140350877193, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=33.8164251207729, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56266; Mature: 56135

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEAEQSLVTLESDKATMDVPSPSAGTVKEV
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEE
KVKVGDTVSEGTLIVVFEGAGDAKAEAPKAEAPQAAAAPAKAEAPKADALAGGGTVEVKV
EEEECCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
PDIGDYKDIPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGTVKEIKVKVGDTVS
CCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCCCCCCEEEEEEECCCCC
EGALIVVLESGDAAPAAAPKAQAPKAEAPKAAPAPAAQASAPAPAPAPAPAPAPAASGRA
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHASPSVRKFARELGVDVGRVTGSGPKGRITKEDVTAFVKGVMTGQTAAPAGAAAPAGGG
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ELNLLPWPKIDFTKFGPVEAQPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRV
EEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHEEHHE
QLNKENEKSGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLILKQYFHIGFAADTPNGL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHEEEECCCCCCE
VVPVIRDADKKGLVDIAKEMADLSKAARDGKLKPDQMQGGCFSISSLGGIGGTHFTPIVN
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCC
APEVAILGLSRSAMKPVWDGKQFVPRLTLPMSLSYDHRVIDGAAAARFNAYLASILGDFR
CCCEEEEECCHHHCCCCCCCCHHCCEEECEEECCCCCEECCCHHHHHHHHHHHHHHHHHH
RVIL
HHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEAEQSLVTLESDKATMDVPSPSAGTVKEV
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEE
KVKVGDTVSEGTLIVVFEGAGDAKAEAPKAEAPQAAAAPAKAEAPKADALAGGGTVEVKV
EEEECCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
PDIGDYKDIPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGTVKEIKVKVGDTVS
CCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCCCCCCEEEEEEECCCCC
EGALIVVLESGDAAPAAAPKAQAPKAEAPKAAPAPAAQASAPAPAPAPAPAPAPAASGRA
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHASPSVRKFARELGVDVGRVTGSGPKGRITKEDVTAFVKGVMTGQTAAPAGAAAPAGGG
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ELNLLPWPKIDFTKFGPVEAQPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRV
EEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHEEHHE
QLNKENEKSGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLILKQYFHIGFAADTPNGL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHEEEECCCCCCE
VVPVIRDADKKGLVDIAKEMADLSKAARDGKLKPDQMQGGCFSISSLGGIGGTHFTPIVN
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCC
APEVAILGLSRSAMKPVWDGKQFVPRLTLPMSLSYDHRVIDGAAAARFNAYLASILGDFR
CCCEEEEECCHHHCCCCCCCCHHCCEEECEEECCCCCEECCCHHHHHHHHHHHHHHHHHH
RVIL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]