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The map label for this gene is emrB [H]

Identifier: 238027581

GI number: 238027581

Start: 2189283

End: 2190845

Strand: Direct

Name: emrB [H]

Synonym: bglu_1g19990

Alternate gene names: 238027581

Gene position: 2189283-2190845 (Clockwise)

Preceding gene: 238027580

Following gene: 238027591

Centisome position: 56.04

GC content: 66.35

Gene sequence:

>1563_bases
ATGGCCAACACCCAACCCGTCATCCACCCTCCGCTGGAGGGCGGTCAGCTACTGCTCGGCACCGTCGCCGTGTCGCTTGC
GGTGTTCATGAACGTGCTCGACACCTCGATCGCCAACGTCGCGATCCCGACCATCTCGGGCGATCTCGGCGTCTCGTCGG
ACCAGGGCACCTGGGTCATCACCTCGTTCGCGGTCGCCAACGCGATCTCGGTGCCGCTGACCGGCTGGCTGACCGACCGG
ATCGGCCAGGTCCGGCTGTTCTTCGCCTCGATCATCCTGTTCGTGATCTCGTCGTGGCTCTGCGGGCTCGCGCCGACGCT
GCCGTTCCTGCTGGTCGCGCGCGTGATCCAGGGCGCCGTGGCCGGGCCGATGATTCCGCTCTCGCAATCGCTGCTGCTGT
CGAGCTATCCACGCGCGCGCGCGCCGATGGCGCTCGCGCTGTGGGCCATGACCACGCTGATCGCCCCCGTGGCCGGGCCG
ATCCTCGGCGGCTGGATCTCCGACAACTACTCGTGGCCGTGGATCTTCTACGTGAACATTCCGGTCGGCATCGCCGCCGC
GATCGTCACCTGGATGATCTACCGCGACCGCGAATCGACCACACGCAAGGCGCCGATCGACGGCGTCGGGCTCGCGCTGC
TGGTGATCTGGGTCGGCTCGCTGCAGATCATGCTCGATAAGGGCAAGGATCTCGACTGGTTCTCCTCGACCACCATCGTG
GCGCTTGCGCTGACCGCCGTCATCGCGTTCGCGTTCTTCGTGATCTGGGAGCTGACGGCCGAGCACCCGGTGGTCGATCT
GTCGCTGTTCGCGATGCGCAACTTCAGCGGCGGCACGATCGCGCTGTCGGTCGGCTACGGGCTCTACTTCGGCAACCTCG
TGCTGCTGCCGCTGTGGCTGCAGACGCAGATCGGCTACACGGCAACCGACGCCGGGCTCGTGATGGCGCCGGTCGGCTTC
TTCGCGATCCTGCTTTCGCCGCTGACGGGCAAGTACCTGCCGCGCACCGATCCGCGCTACATCGCCACCGCCGCGTTCCT
GATCTTCGCGCTGTGCTTCTGGATGCGCTCGCGCTACACCACCGGCGTCGACCAGTGGTCGCTGATGCTGCCGACCTTCG
TGCAGGGCATCGGGATGGCGGGCTTCTTCATCCCGCTCGTCTCGATCACGCTGTCGGGCCTGCCCGGCCATCGGATTCCG
GCCGCCTCGGGCCTGTCGAACTTCGTGCGGATCATGTTCGGCGGCATCGGCACCTCGATCTTCCAGACCGCGTGGGACCA
TCGCAACAACTTCCACCACGCGCAGATCGTCGAGGCGGCCAACGTCTACAACCCGATCTACAACCAGGCAGTCGCGCAGA
TGGGCAGCCTCGGGCTCTCCCAGCAGCAGGCCAACGGGCTCATCAACCAGATGGCCACGCAACAGGCCGCGCAACTCGGC
GTCAACGACCTGTTCTATGTGTCGGCCCTGATCTTCGTGATACTGATCGGCCTGATCTGGATCACCAAGCCCGAACGCGC
GGGCGGCGCGGATTCGAGCGCGGCGGCATCGGCCGCGCATTGA

Upstream 100 bases:

>100_bases
CCGGATCATCGCGCAGAACGCCGGCGGCGCGACGCCGGCCGCCACCAAGCCCGGCGCCGGCGCCGCGCCGAAGCGGATGT
GAGCCCGGAACGGAACGCCC

Downstream 100 bases:

>100_bases
GGCGGCGGCCCGCCTCGGCAAGGCGGTGCGGGCCGCCGCCCTTTGAACAGCACGACGCCCGGCAACGCGAGGCAGCGGGC
CGTCAGCCCGCCGCCTCGCC

Product: EmrB/QacA family protein drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MANTQPVIHPPLEGGQLLLGTVAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDR
IGQVRLFFASIILFVISSWLCGLAPTLPFLLVARVIQGAVAGPMIPLSQSLLLSSYPRARAPMALALWAMTTLIAPVAGP
ILGGWISDNYSWPWIFYVNIPVGIAAAIVTWMIYRDRESTTRKAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFSSTTIV
ALALTAVIAFAFFVIWELTAEHPVVDLSLFAMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGF
FAILLSPLTGKYLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDQWSLMLPTFVQGIGMAGFFIPLVSITLSGLPGHRIP
AASGLSNFVRIMFGGIGTSIFQTAWDHRNNFHHAQIVEAANVYNPIYNQAVAQMGSLGLSQQQANGLINQMATQQAAQLG
VNDLFYVSALIFVILIGLIWITKPERAGGADSSAAASAAH

Sequences:

>Translated_520_residues
MANTQPVIHPPLEGGQLLLGTVAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDR
IGQVRLFFASIILFVISSWLCGLAPTLPFLLVARVIQGAVAGPMIPLSQSLLLSSYPRARAPMALALWAMTTLIAPVAGP
ILGGWISDNYSWPWIFYVNIPVGIAAAIVTWMIYRDRESTTRKAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFSSTTIV
ALALTAVIAFAFFVIWELTAEHPVVDLSLFAMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGF
FAILLSPLTGKYLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDQWSLMLPTFVQGIGMAGFFIPLVSITLSGLPGHRIP
AASGLSNFVRIMFGGIGTSIFQTAWDHRNNFHHAQIVEAANVYNPIYNQAVAQMGSLGLSQQQANGLINQMATQQAAQLG
VNDLFYVSALIFVILIGLIWITKPERAGGADSSAAASAAH
>Mature_519_residues
ANTQPVIHPPLEGGQLLLGTVAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRI
GQVRLFFASIILFVISSWLCGLAPTLPFLLVARVIQGAVAGPMIPLSQSLLLSSYPRARAPMALALWAMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAIVTWMIYRDRESTTRKAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFSSTTIVA
LALTAVIAFAFFVIWELTAEHPVVDLSLFAMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGFF
AILLSPLTGKYLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDQWSLMLPTFVQGIGMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMFGGIGTSIFQTAWDHRNNFHHAQIVEAANVYNPIYNQAVAQMGSLGLSQQQANGLINQMATQQAAQLGV
NDLFYVSALIFVILIGLIWITKPERAGGADSSAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=54.3259557344064, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=495, Percent_Identity=47.0707070707071, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790195, Length=376, Percent_Identity=32.4468085106383, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=30.0995024875622, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081983, Length=394, Percent_Identity=24.8730964467005, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790146, Length=157, Percent_Identity=28.0254777070064, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=197, Percent_Identity=24.8730964467005, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=134, Percent_Identity=32.8358208955224, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=170, Percent_Identity=27.6470588235294, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55811; Mature: 55680

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTQPVIHPPLEGGQLLLGTVAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTDRIGQVRLFFASIILFVISSWLCGLAPTLPFLLVARVIQGAV
EHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPLSQSLLLSSYPRARAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNI
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
PVGIAAAIVTWMIYRDRESTTRKAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFSSTTIV
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
ALALTAVIAFAFFVIWELTAEHPVVDLSLFAMRNFSGGTIALSVGYGLYFGNLVLLPLWL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHH
QTQIGYTATDAGLVMAPVGFFAILLSPLTGKYLPRTDPRYIATAAFLIFALCFWMRSRYT
HHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TGVDQWSLMLPTFVQGIGMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMFGGIGTSI
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FQTAWDHRNNFHHAQIVEAANVYNPIYNQAVAQMGSLGLSQQQANGLINQMATQQAAQLG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VNDLFYVSALIFVILIGLIWITKPERAGGADSSAAASAAH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
ANTQPVIHPPLEGGQLLLGTVAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTDRIGQVRLFFASIILFVISSWLCGLAPTLPFLLVARVIQGAV
EHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPLSQSLLLSSYPRARAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNI
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
PVGIAAAIVTWMIYRDRESTTRKAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFSSTTIV
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
ALALTAVIAFAFFVIWELTAEHPVVDLSLFAMRNFSGGTIALSVGYGLYFGNLVLLPLWL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHH
QTQIGYTATDAGLVMAPVGFFAILLSPLTGKYLPRTDPRYIATAAFLIFALCFWMRSRYT
HHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TGVDQWSLMLPTFVQGIGMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMFGGIGTSI
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FQTAWDHRNNFHHAQIVEAANVYNPIYNQAVAQMGSLGLSQQQANGLINQMATQQAAQLG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VNDLFYVSALIFVILIGLIWITKPERAGGADSSAAASAAH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]