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The map label for this gene is fhlA [H]

Identifier: 238027228

GI number: 238027228

Start: 1767114

End: 1768592

Strand: Direct

Name: fhlA [H]

Synonym: bglu_1g16140

Alternate gene names: 238027228

Gene position: 1767114-1768592 (Clockwise)

Preceding gene: 238027225

Following gene: 238027232

Centisome position: 45.24

GC content: 73.97

Gene sequence:

>1479_bases
ATGCCGCCGTCCGATGCTCCCGCCGCCGATGCCGCCGATGCCGCCAATGCGGCCAGCGCTTCCGATGCGCCCGGCCGTGG
CGGCGCCGCGCGCCGGCCGCACGGTGGGCGGCGCGCCATCCCGATCGTGCCCGCCGATCCGGACCGCGCGCCGCCGCGCG
TCGAGGCGGCCGCCGCGCCCGGGCTCGGCGCGCTCGTGTCGTATCTGGAGCATGATCCGCAGCCGATGATCGTGGTCGAT
CGCGACTATCGGATCCTCGCCGCGAACGGCGCCTATCAGCGCCAGTTCGGCGGCGCGGGCCGCGAGCATGTGGGCCGCCA
CTGCTACCGCGTCTCGCATCATTACGACGTGCCCTGCGACCAGGCCGGCGAGCATTGTCCGATGCGCGCCGCGCTCGAAT
CGGGCACGGCCTCGCGCGTGCTGCACATCCATCACACGCCGCGCGGGCCGGAGCACGTGGACGTCGAGATGCGGCCCGTG
TTCGATGCCGGCGGCGAGGTGATCGCCTACGTCGAGCGGCTGACCACCGTGCGCAGCGCCTCGGCGCGGCCGAGCGCGGG
CGGGCTGGTGGGTAGCTCGGATGCGTTCAACGGCGCGCTGTCGGCGCTGCAGCGCGTGGCCCCGTCGATGCTGCCGGTGC
TGCTGCTCGGCGAATCGGGCACCGGCAAGGAGCTGTTCGCGCGGGCGCTGCACGAGGCCAGCGCGCGGGCGGGCGGGCCG
TTCGTGGTGGTCGATTGCTCGGGGATCGCCGAGTCGCTGTTCGAGAGCGAGCTGTTCGGCTATGAGAAGGGCGCGTTCAC
TGGCGCCAACGCGCGCAAGCCGGGGCTCGTGGAGACGGCGCAGGGCGGCACGCTGTTCGTCGACGAGATCGGCGACGTGC
CCGCCTCGATGCAGGTCAAGCTGCTGCGGCTGATCGAGTCGGGTACGTTTCGCCGCGTCGGCGGCGTGGAGGCGCTGCGC
GCCGACTTCCGGCTCGTCGCGGCCACCCATCAGCCGCTCAGCGAGATGATCGGCGACGGCCGCTTCCGGCAGGATCTCTA
TTACCGGATCAGCGCGTTTCCGATCGAGCTGCCGCCGGTGCGCGAGCGGCCCGGCGACATCGGCCTGCTGGCCGAGTCGA
TCCTGCGCCGCATCGCCGACGCGCGGCCCGGCGCGCGTGCCCACGTGCTGCACGAGACGGCGCGCGCCTGCCTCGACGCC
TATGCCTGGCCGGGCAACATCCGCGAACTGCGCAACGTGCTCGAGCGCGCCTGCCTGTTCGCCGACGACGGCGTGATCCG
CGTCGAGCACCTGCCCGATGCGTTGCGCGAACCGCCCGCCGACGCGGCCGCGCGCGGCGCGCCCGACGACGACACGCTGC
GGCAGGTGGCGCGCACCTTTCGCGGCACCCGCAAGGCGCTGGCGGCGCGGCTCGGCATGAGCGAGCGCACGCTGTATCGG
CGCATGCGGGCGCTGGGGCTGGGCGGGAGCGGGGAGTGA

Upstream 100 bases:

>100_bases
CGAGGCAAAATGCCTCCGAGCGCGGCGCGTTCCACCGCTGTTCCGTTTCGCGTACCGAGCCCGCCCGATGCCGCTTTCCC
CTCCCGCCGAGACGGCGCCT

Downstream 100 bases:

>100_bases
GGGCGCGCGCCGCGGCAAGCGATGCGGCGGCGCCGCACGCGACGCGAGCCAGGCAGGCGCGCGGTGCGCCGCGTCGGCGC
CTCAGGGCTCGAGCACCCGC

Product: Fis family sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MPPSDAPAADAADAANAASASDAPGRGGAARRPHGGRRAIPIVPADPDRAPPRVEAAAAPGLGALVSYLEHDPQPMIVVD
RDYRILAANGAYQRQFGGAGREHVGRHCYRVSHHYDVPCDQAGEHCPMRAALESGTASRVLHIHHTPRGPEHVDVEMRPV
FDAGGEVIAYVERLTTVRSASARPSAGGLVGSSDAFNGALSALQRVAPSMLPVLLLGESGTGKELFARALHEASARAGGP
FVVVDCSGIAESLFESELFGYEKGAFTGANARKPGLVETAQGGTLFVDEIGDVPASMQVKLLRLIESGTFRRVGGVEALR
ADFRLVAATHQPLSEMIGDGRFRQDLYYRISAFPIELPPVRERPGDIGLLAESILRRIADARPGARAHVLHETARACLDA
YAWPGNIRELRNVLERACLFADDGVIRVEHLPDALREPPADAAARGAPDDDTLRQVARTFRGTRKALAARLGMSERTLYR
RMRALGLGGSGE

Sequences:

>Translated_492_residues
MPPSDAPAADAADAANAASASDAPGRGGAARRPHGGRRAIPIVPADPDRAPPRVEAAAAPGLGALVSYLEHDPQPMIVVD
RDYRILAANGAYQRQFGGAGREHVGRHCYRVSHHYDVPCDQAGEHCPMRAALESGTASRVLHIHHTPRGPEHVDVEMRPV
FDAGGEVIAYVERLTTVRSASARPSAGGLVGSSDAFNGALSALQRVAPSMLPVLLLGESGTGKELFARALHEASARAGGP
FVVVDCSGIAESLFESELFGYEKGAFTGANARKPGLVETAQGGTLFVDEIGDVPASMQVKLLRLIESGTFRRVGGVEALR
ADFRLVAATHQPLSEMIGDGRFRQDLYYRISAFPIELPPVRERPGDIGLLAESILRRIADARPGARAHVLHETARACLDA
YAWPGNIRELRNVLERACLFADDGVIRVEHLPDALREPPADAAARGAPDDDTLRQVARTFRGTRKALAARLGMSERTLYR
RMRALGLGGSGE
>Mature_491_residues
PPSDAPAADAADAANAASASDAPGRGGAARRPHGGRRAIPIVPADPDRAPPRVEAAAAPGLGALVSYLEHDPQPMIVVDR
DYRILAANGAYQRQFGGAGREHVGRHCYRVSHHYDVPCDQAGEHCPMRAALESGTASRVLHIHHTPRGPEHVDVEMRPVF
DAGGEVIAYVERLTTVRSASARPSAGGLVGSSDAFNGALSALQRVAPSMLPVLLLGESGTGKELFARALHEASARAGGPF
VVVDCSGIAESLFESELFGYEKGAFTGANARKPGLVETAQGGTLFVDEIGDVPASMQVKLLRLIESGTFRRVGGVEALRA
DFRLVAATHQPLSEMIGDGRFRQDLYYRISAFPIELPPVRERPGDIGLLAESILRRIADARPGARAHVLHETARACLDAY
AWPGNIRELRNVLERACLFADDGVIRVEHLPDALREPPADAAARGAPDDDTLRQVARTFRGTRKALAARLGMSERTLYRR
MRALGLGGSGE

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=40.8955223880597, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1788550, Length=324, Percent_Identity=39.1975308641975, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1790437, Length=304, Percent_Identity=43.0921052631579, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082117, Length=324, Percent_Identity=40.4320987654321, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1790299, Length=360, Percent_Identity=37.7777777777778, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=37.797619047619, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=42.1052631578947, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=263, Percent_Identity=43.7262357414449, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=39.7476340694006, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1788905, Length=287, Percent_Identity=39.7212543554007, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=444, Percent_Identity=32.8828828828829, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081858, Length=293, Percent_Identity=33.1058020477816, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789828, Length=302, Percent_Identity=34.1059602649007, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52462; Mature: 52331

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSDAPAADAADAANAASASDAPGRGGAARRPHGGRRAIPIVPADPDRAPPRVEAAAAP
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCC
GLGALVSYLEHDPQPMIVVDRDYRILAANGAYQRQFGGAGREHVGRHCYRVSHHYDVPCD
CHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHEECCCCCCCCCC
QAGEHCPMRAALESGTASRVLHIHHTPRGPEHVDVEMRPVFDAGGEVIAYVERLTTVRSA
CCCCCCCHHHHHHCCCCCEEEEEEECCCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHC
SARPSAGGLVGSSDAFNGALSALQRVAPSMLPVLLLGESGTGKELFARALHEASARAGGP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
FVVVDCSGIAESLFESELFGYEKGAFTGANARKPGLVETAQGGTLFVDEIGDVPASMQVK
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHH
LLRLIESGTFRRVGGVEALRADFRLVAATHQPLSEMIGDGRFRQDLYYRISAFPIELPPV
HHHHHHCCCCHHHCCHHHHHHHHHHEEHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCC
RERPGDIGLLAESILRRIADARPGARAHVLHETARACLDAYAWPGNIRELRNVLERACLF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
ADDGVIRVEHLPDALREPPADAAARGAPDDDTLRQVARTFRGTRKALAARLGMSERTLYR
CCCCEEEECCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RMRALGLGGSGE
HHHHHCCCCCCC
>Mature Secondary Structure 
PPSDAPAADAADAANAASASDAPGRGGAARRPHGGRRAIPIVPADPDRAPPRVEAAAAP
CCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCC
GLGALVSYLEHDPQPMIVVDRDYRILAANGAYQRQFGGAGREHVGRHCYRVSHHYDVPCD
CHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHEECCCCCCCCCC
QAGEHCPMRAALESGTASRVLHIHHTPRGPEHVDVEMRPVFDAGGEVIAYVERLTTVRSA
CCCCCCCHHHHHHCCCCCEEEEEEECCCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHC
SARPSAGGLVGSSDAFNGALSALQRVAPSMLPVLLLGESGTGKELFARALHEASARAGGP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
FVVVDCSGIAESLFESELFGYEKGAFTGANARKPGLVETAQGGTLFVDEIGDVPASMQVK
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHH
LLRLIESGTFRRVGGVEALRADFRLVAATHQPLSEMIGDGRFRQDLYYRISAFPIELPPV
HHHHHHCCCCHHHCCHHHHHHHHHHEEHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCC
RERPGDIGLLAESILRRIADARPGARAHVLHETARACLDAYAWPGNIRELRNVLERACLF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
ADDGVIRVEHLPDALREPPADAAARGAPDDDTLRQVARTFRGTRKALAARLGMSERTLYR
CCCCEEEECCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RMRALGLGGSGE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]