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The map label for this gene is potF [H]

Identifier: 238027222

GI number: 238027222

Start: 1760295

End: 1761389

Strand: Direct

Name: potF [H]

Synonym: bglu_1g16080

Alternate gene names: 238027222

Gene position: 1760295-1761389 (Clockwise)

Preceding gene: 238027219

Following gene: 238027223

Centisome position: 45.06

GC content: 62.65

Gene sequence:

>1095_bases
ATGAAAGCAAAGGTGGCAGGCCGGTTCGTGGCGCTCTCGCTTTGTGTGGCCGCGTCGGCGGCTGCGGCGAAGGATACGCA
GCTCAACGTGTACAACTGGTCCGACTACATTGCCAAGGACACGATCCCGAACTTCGAGAAACAGACGGGCGTGAAGGTCC
GTTACGACAACTACGACAGCGACGACACGCTGCAGGCCAAGCTGCTCACCGGCAACTCGGGCTACGACATCGTGGTGCCG
ACCAGCAACTACGCGGGCAAGCAGATTGCTGCCGGCATCTTCGCGCCGCTCGACAAATCGAAGCTGCCGAACCTCAAGAA
CCTCGATCCGCAGCTGATGGCGCTGGTGGCCGGTGCCGATCCCGGCAACAAGTACGTGGTGCCCTGGGCCTACGGCACCA
CGGGCCTCGGCTACAACGTGAACAAGGCGCAGCAGATCCTCGGCAAGGTGCCGCTCGACAGCTGGGACATCCTGTTCAAG
CCGGAAAACATCTCGAAGCTGAAGACGTGCGGCGTGTCGGTGCTCGACGCGCCCGACCAGATGTTCGCCGCCACGTTGCA
CTACATCGGCAAGGATCCGATGAGCACCAACCCGGCCGACTATCAGGCCGCGATGCAGGTGCTCAAGAAGATCCGCCCGT
ACATCACGCAGTTCAACTCGTCGGGCTATATCAACGACCTGGTCGGCGGCGACATCTGCTTCGCGTTCGGCTGGTCCGGC
GACGTCGTGATCGCCAAGCATCGCGCGCAGGAAGCGAAGAAACCGTACAAGGTCGAGTACTACGTGCCGAAGGGCGGCGC
GCCGGTCTGGTTCGACGTGATGGCGATCCCGAAGGACGCGAAGCACAAGGAAGCGGCTCTCGAATGGATCAACTACATCG
AGGATCCGAAGGTCAACGCGGCGATCACGAACGCGGTCTATTACCCGAGCGCCAACGCGGCGGCGCGCAAGTTCGTGAAC
CCGGACGTGGCCAACGATCCGGCCGTCTATCCGCCCGCCGACGTGGTCAAGACGCTGTTCCTGCTCAAGCCGCTGCCGCC
GGAAATCCAGCGCCTGCAGACCCGGCTCTGGACGGAACTGAAATCGGGGCGCTGA

Upstream 100 bases:

>100_bases
TTAGTGATACTTTTCGGTGTTTTAGGGGACGGGTCGCGAGGGCGCGGGCCGTCGTGACGACAAGTCATACCGGCCGCACA
CGGCCGGACGGGAGAACGGG

Downstream 100 bases:

>100_bases
GCGCCAGCCCGGAGCACCGGCGCCGATCCACCAAGCCCCTGGCAACCCCGGGGGCTTTGTTCGTCTTCGCGCGCCCTTGC
CGAGGCGCATCGCCGTCCGT

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKAKVAGRFVALSLCVAASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGNSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKNLDPQLMALVAGADPGNKYVVPWAYGTTGLGYNVNKAQQILGKVPLDSWDILFK
PENISKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYQAAMQVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAQEAKKPYKVEYYVPKGGAPVWFDVMAIPKDAKHKEAALEWINYIEDPKVNAAITNAVYYPSANAAARKFVN
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Sequences:

>Translated_364_residues
MKAKVAGRFVALSLCVAASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGNSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKNLDPQLMALVAGADPGNKYVVPWAYGTTGLGYNVNKAQQILGKVPLDSWDILFK
PENISKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYQAAMQVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAQEAKKPYKVEYYVPKGGAPVWFDVMAIPKDAKHKEAALEWINYIEDPKVNAAITNAVYYPSANAAARKFVN
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR
>Mature_364_residues
MKAKVAGRFVALSLCVAASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDSDDTLQAKLLTGNSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKNLDPQLMALVAGADPGNKYVVPWAYGTTGLGYNVNKAQQILGKVPLDSWDILFK
PENISKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYQAAMQVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAQEAKKPYKVEYYVPKGGAPVWFDVMAIPKDAKHKEAALEWINYIEDPKVNAAITNAVYYPSANAAARKFVN
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=50.828729281768, Blast_Score=386, Evalue=1e-109,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=36.4485981308411, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=24.4514106583072, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39931; Mature: 39931

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKVAGRFVALSLCVAASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDS
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCCC
DDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKNLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCCCHHHEEEEECCC
PGNKYVVPWAYGTTGLGYNVNKAQQILGKVPLDSWDILFKPENISKLKTCGVSVLDAPDQ
CCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCEEECCCHH
MFAATLHYIGKDPMSTNPADYQAAMQVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCC
DVVIAKHRAQEAKKPYKVEYYVPKGGAPVWFDVMAIPKDAKHKEAALEWINYIEDPKVNA
CEEEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
AITNAVYYPSANAAARKFVNPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC
>Mature Secondary Structure
MKAKVAGRFVALSLCVAASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQTGVKVRYDNYDS
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCCC
DDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKNLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCCCHHHEEEEECCC
PGNKYVVPWAYGTTGLGYNVNKAQQILGKVPLDSWDILFKPENISKLKTCGVSVLDAPDQ
CCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCEEECCCHH
MFAATLHYIGKDPMSTNPADYQAAMQVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCC
DVVIAKHRAQEAKKPYKVEYYVPKGGAPVWFDVMAIPKDAKHKEAALEWINYIEDPKVNA
CEEEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
AITNAVYYPSANAAARKFVNPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]