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The map label for this gene is nuoE2 [H]

Identifier: 238026516

GI number: 238026516

Start: 935066

End: 935554

Strand: Reverse

Name: nuoE2 [H]

Synonym: bglu_1g08600

Alternate gene names: 238026516

Gene position: 935554-935066 (Counterclockwise)

Preceding gene: 238026519

Following gene: 238026515

Centisome position: 23.95

GC content: 70.55

Gene sequence:

>489_bases
ATGTCCCCATCCCCCGTTTCAGCGGACGCGCTCGTCTCGCGGCATGTACGCGCCGGCCGATCGCTCGTGGCAATTCTGCA
CGCCATTCAGGATGAAGCCGGCTACGTGCCCGAGGGCTGCGTGCCGCCGCTCGCGAAAGCGCTGAACCTGTCACGCGCCG
AAGTGCATGGCGTGCTCACCTACTACCACCATTTCCGGACCACGCCGCCCGCGCGCGTGACGATCCAGCTGTGCCGCGCC
GAAGCCTGCCGCAGCCTCGGCGGCGAGGCGCTGGTCGAGCACGCGCAGGCGCGCACCGGCTGCCGAATCGACGCCGGGCA
CAACGGCGACGTCGCGCTCGAATCGGTGTACTGCCTCGGCTTCTGCGCGCAGTCGCCATCCGCGATGATCAACGGCGAGC
CGCATGCGCGCCTGAGCCCGGCGCGCTTCGACGCGCTGCTCGCCGCAGCCGTGGCCGACACCGCCGCCGACCTGAAGGAG
CCCGTATGA

Upstream 100 bases:

>100_bases
CTGGATTCTAACGGCTTATCGTCTTCTGCACTCGATCAAACAACAGCGTCGATGCCGGGATATATGAAACCCCGAGCATA
TAGACCTGGAGGAGCTCAGG

Downstream 100 bases:

>100_bases
GCCAGTCCGCCGTCCGCATCCATGTGCCACGCGACTCGGCCGCGCTGGCGGTCGGCGCCGAGGCGCTGGCCCGCGCGATC
ACGGCCGAAGCCGCCGCGCG

Product: formate dehydrogenase subunit gamma

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSPSPVSADALVSRHVRAGRSLVAILHAIQDEAGYVPEGCVPPLAKALNLSRAEVHGVLTYYHHFRTTPPARVTIQLCRA
EACRSLGGEALVEHAQARTGCRIDAGHNGDVALESVYCLGFCAQSPSAMINGEPHARLSPARFDALLAAAVADTAADLKE
PV

Sequences:

>Translated_162_residues
MSPSPVSADALVSRHVRAGRSLVAILHAIQDEAGYVPEGCVPPLAKALNLSRAEVHGVLTYYHHFRTTPPARVTIQLCRA
EACRSLGGEALVEHAQARTGCRIDAGHNGDVALESVYCLGFCAQSPSAMINGEPHARLSPARFDALLAAAVADTAADLKE
PV
>Mature_161_residues
SPSPVSADALVSRHVRAGRSLVAILHAIQDEAGYVPEGCVPPLAKALNLSRAEVHGVLTYYHHFRTTPPARVTIQLCRAE
ACRSLGGEALVEHAQARTGCRIDAGHNGDVALESVYCLGFCAQSPSAMINGEPHARLSPARFDALLAAAVADTAADLKEP
V

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788621, Length=128, Percent_Identity=28.90625, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17059; Mature: 16927

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSPVSADALVSRHVRAGRSLVAILHAIQDEAGYVPEGCVPPLAKALNLSRAEVHGVLT
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
YYHHFRTTPPARVTIQLCRAEACRSLGGEALVEHAQARTGCRIDAGHNGDVALESVYCLG
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
FCAQSPSAMINGEPHARLSPARFDALLAAAVADTAADLKEPV
HHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SPSPVSADALVSRHVRAGRSLVAILHAIQDEAGYVPEGCVPPLAKALNLSRAEVHGVLT
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
YYHHFRTTPPARVTIQLCRAEACRSLGGEALVEHAQARTGCRIDAGHNGDVALESVYCLG
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
FCAQSPSAMINGEPHARLSPARFDALLAAAVADTAADLKEPV
HHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]