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The map label for this gene is citA [H]

Identifier: 238026510

GI number: 238026510

Start: 925001

End: 926299

Strand: Direct

Name: citA [H]

Synonym: bglu_1g08540

Alternate gene names: 238026510

Gene position: 925001-926299 (Clockwise)

Preceding gene: 238026509

Following gene: 238026512

Centisome position: 23.68

GC content: 67.05

Gene sequence:

>1299_bases
ATGTCAACTGCGACTCACACGCACGCCACTCAGGAATCCAAGGCCCGCTCCGTATTCCGCGTGGTCAGCGGCAACTTCCT
GGAAATGTACGATTTCATGGTCTACGGCTATTACGCCTCGGCGATCGCCAAGACCTACTTTCCGAACGGCGACGCGTTCG
CCTCGCTGATGCTGTCGCTGTCGGTGTTCGGCGCCGGCTTCCTGGTGCGCCCGATCGGCGCGATCGTGCTCGGCGCGTAC
ATCGACCACCACGGCCGCCGCAAGGGGCTGATCCTCACGCTGAGCCTGATGGCGCTCGGCACGCTGGCGGTCGCGACCGT
GCCCGGCTACGCGACCATCGGCGTGGTCGCGCCGATCATCGTGCTGCTCGGGCGGCTGTTGCAGGGCTTTTCGGCCGGGG
TCGAGCTGGGCGGCGTGTCGGTCTACCTGTCGGAGATCGCGACGAAGGGCAACAAGGGCTTCTATACGTCGTGGCAGTCG
GGCAGCCAGCAGGTGGCGGTGGTGTTCGCGGCGCTGGTGGGAGTGCTGCTGCACAGCGCGCTGCCGGTCGAGCAGATGAC
GGCCTGGGGCTGGCGCGTGCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGCTGAGGG
AAACCGACGAGTTTCTCGCCAAGCGCCACCGTCCGAGCATGGGCGAGATCATGCGGTCGATGGTGCAGAACTGGGGCGTC
GTGCTGGCCGGGATGGGCATGGTGATCATGACCACCGTGTCGTTCTACATGATCACGGCCTACACGCCGACCTTCGGCAA
GGAAGAACTGCACTTGTCGGCTGTCGACGCGCTCGTCGTGACGGTCTGCGTCGGCCTGTCGAACCTGTTCTGGCTGCCGC
TGTCGGGCAGCGTGTCGGACCGCATCGGCCGCCGGCCGGTGCTGATCACCTTCACCGTGCTGACGCTGCTGACCGCCTAT
CCGGCCGTGCAGTGGCTGGTGGCCGAGCCGTCGTTCGCGCGCCTGCTGACCGTGGAGCTGTGGCTGTCGTTCCTGTACGG
CTCGTACAACGGCGCGATGGTGGTCGCGCTGACCGAGGTGATGCCGGCCGACGTGCGCACCGCCGGCTTCTCGCTCGCCT
ACAGTCTCGCCACGACGATCGGCGGCTTCACGCCGGCGATCTCGACGCTGCTGATCCACGAGACCCACAACAAGGCGGCG
CCTGGGCTCTGGCTCGGCGTGGCGGCGATCTGCGGGCTGATCGCGACGCTGGTGCTGTACCGCACGCCGGAGGCGCGCCG
GCAGTACAAGGGGGTCTGA

Upstream 100 bases:

>100_bases
CGCCGCGCGGCGCGGTGCCGGTCCGCTACCGCATTCGGCCGCCGTGCCAGCTTCGGTCGCATCGCGACGGCGCACGGCCC
ACGCTCTATCGAGATCAATC

Downstream 100 bases:

>100_bases
GGGCGCCGCGGTGTGCCGTGAGGGCATGTGGAACAGGGGGCGCCGCAGCGCTCCCTTTTCGTTTCGAGACGGGCGGCGAG
GTTGCCCGTCGCGCGCGTGC

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSTATHTHATQESKARSVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPNGDAFASLMLSLSVFGAGFLVRPIGAIVLGAY
IDHHGRRKGLILTLSLMALGTLAVATVPGYATIGVVAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQS
GSQQVAVVFAALVGVLLHSALPVEQMTAWGWRVPFLIGCLIVPFLFLIRRSLRETDEFLAKRHRPSMGEIMRSMVQNWGV
VLAGMGMVIMTTVSFYMITAYTPTFGKEELHLSAVDALVVTVCVGLSNLFWLPLSGSVSDRIGRRPVLITFTVLTLLTAY
PAVQWLVAEPSFARLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETHNKAA
PGLWLGVAAICGLIATLVLYRTPEARRQYKGV

Sequences:

>Translated_432_residues
MSTATHTHATQESKARSVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPNGDAFASLMLSLSVFGAGFLVRPIGAIVLGAY
IDHHGRRKGLILTLSLMALGTLAVATVPGYATIGVVAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQS
GSQQVAVVFAALVGVLLHSALPVEQMTAWGWRVPFLIGCLIVPFLFLIRRSLRETDEFLAKRHRPSMGEIMRSMVQNWGV
VLAGMGMVIMTTVSFYMITAYTPTFGKEELHLSAVDALVVTVCVGLSNLFWLPLSGSVSDRIGRRPVLITFTVLTLLTAY
PAVQWLVAEPSFARLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETHNKAA
PGLWLGVAAICGLIATLVLYRTPEARRQYKGV
>Mature_431_residues
STATHTHATQESKARSVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPNGDAFASLMLSLSVFGAGFLVRPIGAIVLGAYI
DHHGRRKGLILTLSLMALGTLAVATVPGYATIGVVAPIIVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSG
SQQVAVVFAALVGVLLHSALPVEQMTAWGWRVPFLIGCLIVPFLFLIRRSLRETDEFLAKRHRPSMGEIMRSMVQNWGVV
LAGMGMVIMTTVSFYMITAYTPTFGKEELHLSAVDALVVTVCVGLSNLFWLPLSGSVSDRIGRRPVLITFTVLTLLTAYP
AVQWLVAEPSFARLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETHNKAAP
GLWLGVAAICGLIATLVLYRTPEARRQYKGV

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=29.8850574712644, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=31.2941176470588, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788292, Length=391, Percent_Identity=28.3887468030691, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=29.2792792792793, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.9911504424779, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46714; Mature: 46582

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHCCCC
>Mature Secondary Structure 
STATHTHATQESKARSVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPNGDAFASLMLSL
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SVFGAGFLVRPIGAIVLGAYIDHHGRRKGLILTLSLMALGTLAVATVPGYATIGVVAPII
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGSQQVAVVFAALVGVLLHSA
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCC
LPVEQMTAWGWRVPFLIGCLIVPFLFLIRRSLRETDEFLAKRHRPSMGEIMRSMVQNWGV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
VLAGMGMVIMTTVSFYMITAYTPTFGKEELHLSAVDALVVTVCVGLSNLFWLPLSGSVSD
HHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHH
RIGRRPVLITFTVLTLLTAYPAVQWLVAEPSFARLLTVELWLSFLYGSYNGAMVVALTEV
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
MPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETHNKAAPGLWLGVAAICGLIATLVLY
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RTPEARRQYKGV
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]