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The map label for this gene is capD [H]

Identifier: 238026407

GI number: 238026407

Start: 804160

End: 806043

Strand: Direct

Name: capD [H]

Synonym: bglu_1g07420

Alternate gene names: 238026407

Gene position: 804160-806043 (Clockwise)

Preceding gene: 238026406

Following gene: 238026413

Centisome position: 20.59

GC content: 69.16

Gene sequence:

>1884_bases
ATGATTCGACCCAAAGCATCCTGGCTCTCGTTCGGCGCGTTCCTGTTCGACGTGCTGGCCGTGGCGGCCGCCTGGCTTGT
CAGCTATCTGGTCCGTTTCAACGGCGAGATCCCCCCTGAGTTTTTCCACGGCGGCGTGACCGCACTCGCCTGGGTGCTGC
CCGTCTACGCCGTGCTGTTCCGCGCCCTCGGCCTCTATCGCGGGCTGTGGGTATTCGCGAGCCTGCCCGACCTGCTGCGG
ATCTCGAAGGCCGTGATGGGCGGCGGCGGCATCGTGATGGCCTGCGCCGTGATGTTCCAGCCGGCACCCATCATTCCGCG
CTCGGTGCTGCTGCTCTCGCCGATGCTGCTGTTCCTCGTGATGGGCGGCGCGCGCGCGCTGTACCGTGCGATCAAGGAGT
TCTATCGCTACGGCGGCCTCGTCGGCCAGGGCAAGCCGGTGCTGGTGCTCGGCGCGGGCGGCGCCGGCGCGAGCCTCGCG
CGCGAGCTGTCGCGCTCGGGCGAATGGCGGCTGGTGGGCCTGCTCGACGACGAGCCGGCCAAGCGCGGCCGCGAGGTGTA
CGGCTACAAGGTGCTCGGGCCGATTTCCGAACTGCCGCGCTGGGCCGAGGCGACGAAGGCCGAGCACGTGATCATCGCGA
TGCCGTCGGCCTCGGTGGAGGTGCAGCGCCGCGTCGCCACGTTGTGCGTGCGGGCGGGCGTGAAGGCGATGGTCCTGCCG
TCGTTGACCGGGCTGATGCCCGGGCAGGGCTTCCTGTCCCAGGTGCGGAGCATCAACCTCGAGGACCTGCTCGGCCGCGA
CGAGGTGACGATCGACACGGCCCACGTCGACGCCCTGCTGCACGATCGCGTGGTGATGGTGACGGGCGCGGGCGGCTCGA
TCGGCTCCGAGCTGTGCCGCCAGATCCTGCGCTTCTCGCCCGCGCAGCTGATCGCGTTCGACCTGTCCGAATTCGCGATC
TACCGGCTCACCGAGGACCTGCGCGAGCGCTTCGGCGACAGCCGCGTGGTGCCGATCATCGGCGACGCCAAGGATTCGCT
GCTGCTCGAGCACGTGATGTCGAGCTACGCGCCGCACATCGTGTTCCATGCGGCCGCCTACAAGCACGTGCCGCTGATGG
AGGAGCTCAACGCCTGGCAGGCGCTGCGCAACAACGTGCTCGGCACCTACCGCGTGGCGCGCGCGGCGATCCGCCATGGC
GTGCGCCACTTCGTGCTGATCTCCACCGACAAGGCCGTCAATCCGACCAACGTGATGGGGGCCAGCAAGCGGCTCGCCGA
GATGGCCTGCCAGGCGCTGCAGCAGACCAGCCCGAACACGCAGTTCGAGACGGTGCGCTTCGGCAACGTGCTCGGCAGCG
CCGGCAGCGTGATCCCGAAGTTCCAGCAGCAGATCGCCAAGGGCGGCCCGGTGACGGTCACGCACCCCGAGATCACGCGC
TTCTTCATGACGATTCCCGAGGCCTCGCAACTCGTGCTGCAGGCCTCGAGCATGGGGCAGGGCGGCGAGATCTTCATCCT
CGACATGGGCGAGCCGGTGCGCATCGTCGATCTGGCGCGCGACCTGATCCGCCTCTACGGCTTCGACGAACAGCAGATCC
GCATCGAGTTCACGGGCCTGCGGCCCGGCGAGAAGCTCTACGAGGAACTGCTCGCCGACGACGAGACCACCACCCGCACG
CCGCATCCGAAGCTGCGCACCGCGAAGGCGCGCGAGGTGCCCGACCACCTGCTCGACGAATTGCTGCCTTGGCTCATGCA
GCATCGCGTGCTCGGCGACGACGAGGTGCGCCGCGACCTGCGCCGCTGGGTGCCCGAGTATCAGCCGGCCTGCGCGCCGA
CCTTGCAGAGCATCGCCGGCGGCGGCGCGCAGCAGGCCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCTCTGGTATCTCGTGCTCGGCGCGCTGGCGCTGGCGATTGACGCGCGGTGGCGACGCTATCAGGCGGCGCTGTCA
TCTACTCGAGGTATGCGCCC

Downstream 100 bases:

>100_bases
CAGGTCCGGCCCGTATGTGAGGGGGCGGCGGCAAAGCGAAAGGCGCTCGAACGAGCGCCTTTTTTCATGGTGAAGCCGGC
GCCGCGCGGCACGCCGCCCG

Product: Capsular polysaccharide biosynthesis

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MIRPKASWLSFGAFLFDVLAVAAAWLVSYLVRFNGEIPPEFFHGGVTALAWVLPVYAVLFRALGLYRGLWVFASLPDLLR
ISKAVMGGGGIVMACAVMFQPAPIIPRSVLLLSPMLLFLVMGGARALYRAIKEFYRYGGLVGQGKPVLVLGAGGAGASLA
RELSRSGEWRLVGLLDDEPAKRGREVYGYKVLGPISELPRWAEATKAEHVIIAMPSASVEVQRRVATLCVRAGVKAMVLP
SLTGLMPGQGFLSQVRSINLEDLLGRDEVTIDTAHVDALLHDRVVMVTGAGGSIGSELCRQILRFSPAQLIAFDLSEFAI
YRLTEDLRERFGDSRVVPIIGDAKDSLLLEHVMSSYAPHIVFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHG
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPNTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGQGGEIFILDMGEPVRIVDLARDLIRLYGFDEQQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRTAKAREVPDHLLDELLPWLMQHRVLGDDEVRRDLRRWVPEYQPACAPTLQSIAGGGAQQAG

Sequences:

>Translated_627_residues
MIRPKASWLSFGAFLFDVLAVAAAWLVSYLVRFNGEIPPEFFHGGVTALAWVLPVYAVLFRALGLYRGLWVFASLPDLLR
ISKAVMGGGGIVMACAVMFQPAPIIPRSVLLLSPMLLFLVMGGARALYRAIKEFYRYGGLVGQGKPVLVLGAGGAGASLA
RELSRSGEWRLVGLLDDEPAKRGREVYGYKVLGPISELPRWAEATKAEHVIIAMPSASVEVQRRVATLCVRAGVKAMVLP
SLTGLMPGQGFLSQVRSINLEDLLGRDEVTIDTAHVDALLHDRVVMVTGAGGSIGSELCRQILRFSPAQLIAFDLSEFAI
YRLTEDLRERFGDSRVVPIIGDAKDSLLLEHVMSSYAPHIVFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHG
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPNTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGQGGEIFILDMGEPVRIVDLARDLIRLYGFDEQQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRTAKAREVPDHLLDELLPWLMQHRVLGDDEVRRDLRRWVPEYQPACAPTLQSIAGGGAQQAG
>Mature_627_residues
MIRPKASWLSFGAFLFDVLAVAAAWLVSYLVRFNGEIPPEFFHGGVTALAWVLPVYAVLFRALGLYRGLWVFASLPDLLR
ISKAVMGGGGIVMACAVMFQPAPIIPRSVLLLSPMLLFLVMGGARALYRAIKEFYRYGGLVGQGKPVLVLGAGGAGASLA
RELSRSGEWRLVGLLDDEPAKRGREVYGYKVLGPISELPRWAEATKAEHVIIAMPSASVEVQRRVATLCVRAGVKAMVLP
SLTGLMPGQGFLSQVRSINLEDLLGRDEVTIDTAHVDALLHDRVVMVTGAGGSIGSELCRQILRFSPAQLIAFDLSEFAI
YRLTEDLRERFGDSRVVPIIGDAKDSLLLEHVMSSYAPHIVFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHG
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPNTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGQGGEIFILDMGEPVRIVDLARDLIRLYGFDEQQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRTAKAREVPDHLLDELLPWLMQHRVLGDDEVRRDLRRWVPEYQPACAPTLQSIAGGGAQQAG

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 68814; Mature: 68814

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPKASWLSFGAFLFDVLAVAAAWLVSYLVRFNGEIPPEFFHGGVTALAWVLPVYAVLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHH
RALGLYRGLWVFASLPDLLRISKAVMGGGGIVMACAVMFQPAPIIPRSVLLLSPMLLFLV
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MGGARALYRAIKEFYRYGGLVGQGKPVLVLGAGGAGASLARELSRSGEWRLVGLLDDEPA
HCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCHH
KRGREVYGYKVLGPISELPRWAEATKAEHVIIAMPSASVEVQRRVATLCVRAGVKAMVLP
HCCCCCCCEEEECCHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHH
SLTGLMPGQGFLSQVRSINLEDLLGRDEVTIDTAHVDALLHDRVVMVTGAGGSIGSELCR
HHHHCCCCHHHHHHHHCCCHHHHCCCCCEEEEHHHHHHHHHCCEEEEECCCCCHHHHHHH
QILRFSPAQLIAFDLSEFAIYRLTEDLRERFGDSRVVPIIGDAKDSLLLEHVMSSYAPHI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHH
VFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHGVRHFVLISTDKAVNPTNVMG
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHH
ASKRLAEMACQALQQTSPNTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH
FFMTIPEASQLVLQASSMGQGGEIFILDMGEPVRIVDLARDLIRLYGFDEQQIRIEFTGL
HHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
RPGEKLYEELLADDETTTRTPHPKLRTAKAREVPDHLLDELLPWLMQHRVLGDDEVRRDL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RRWVPEYQPACAPTLQSIAGGGAQQAG
HHHCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIRPKASWLSFGAFLFDVLAVAAAWLVSYLVRFNGEIPPEFFHGGVTALAWVLPVYAVLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHH
RALGLYRGLWVFASLPDLLRISKAVMGGGGIVMACAVMFQPAPIIPRSVLLLSPMLLFLV
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MGGARALYRAIKEFYRYGGLVGQGKPVLVLGAGGAGASLARELSRSGEWRLVGLLDDEPA
HCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCHH
KRGREVYGYKVLGPISELPRWAEATKAEHVIIAMPSASVEVQRRVATLCVRAGVKAMVLP
HCCCCCCCEEEECCHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHH
SLTGLMPGQGFLSQVRSINLEDLLGRDEVTIDTAHVDALLHDRVVMVTGAGGSIGSELCR
HHHHCCCCHHHHHHHHCCCHHHHCCCCCEEEEHHHHHHHHHCCEEEEECCCCCHHHHHHH
QILRFSPAQLIAFDLSEFAIYRLTEDLRERFGDSRVVPIIGDAKDSLLLEHVMSSYAPHI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHH
VFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHGVRHFVLISTDKAVNPTNVMG
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHH
ASKRLAEMACQALQQTSPNTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH
FFMTIPEASQLVLQASSMGQGGEIFILDMGEPVRIVDLARDLIRLYGFDEQQIRIEFTGL
HHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
RPGEKLYEELLADDETTTRTPHPKLRTAKAREVPDHLLDELLPWLMQHRVLGDDEVRRDL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RRWVPEYQPACAPTLQSIAGGGAQQAG
HHHCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]