Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 238026282

GI number: 238026282

Start: 648233

End: 649459

Strand: Reverse

Name: glcF [H]

Synonym: bglu_1g06150

Alternate gene names: 238026282

Gene position: 649459-648233 (Counterclockwise)

Preceding gene: 238026283

Following gene: 238026281

Centisome position: 16.63

GC content: 71.56

Gene sequence:

>1227_bases
ATGCAGACGAATCTCGCCGACTTCATCCGCGGCACCCCCGACGGCGACGAAGCCGAATCGATCCTGCGCAAGTGCGTCCA
TTGCGGCTTCTGCACGGCGACCTGCCCCACCTATCAGCTGCTCGGCGACGAGCTCGACGGCCCGCGCGGGCGCATCTACC
TGATCAAGCAGACGCTCGAAGGCGCGCCCGTCACGCGCAGCACGCAGCAGCATCTCGACCGCTGCCTGACCTGCCGCAGC
TGCGAGAGCACCTGCCCGTCGGGCGTGCAGTACGGGCGGCTGGTCGAGATCGGCCGCCAGCAGGTGGACGCGCGCGTGCC
GCGCCCGCTGTCGCAGCGGCTCGTGCGCCGCGCGCTCGCGAGCTTCGTGCCGAACGCCGCGCTGTTCGCGCCGGCCATGC
GGCTCGGCCAGCATTTGCGCCCGCTGCTGCCGCGGCGGCTGCGCGACAAGGTGCCGGCCCGGACCCGGCCGCTCGCATGG
CCGAGCGCGCGCCATCCGCGCAAGATGCTGATGCTGGCCGGCTGCGTGCAGCCCGCGATGATGCCGAACGTCAACATCGC
GACGGCGCGCGTGCTCGACGCGCTCGGCATCGAGACCGTGATCGCGCCCGAGGCCGGCTGCTGCGGCGCGATCCGGCTGC
ATCTGAACTACCGCGACGAGGCGCTCGCCGACGCACGCCGCAACATCGACGCGTGGTGGCCGTTCATCGAGCAGGGCGCC
GAGGCGATCGTGATGAACGCGAGCGGCTGCGGCGCGACCGTGCTCGAGTACGCGCACCTGCTGCGCGACGACGCCGCCTA
CGCGGACAAGGCGCGCCGCATCGTCGAGCTGACCCAGGACGTGTCCGGGCTGCTGGCCGGCTTCGCGCCGCAGCTCGTCG
CGCTCGCCCGGCACCGCGCGAGGCAGACCGTCGCCTACCATCCGCCCTGCACGCTGCAGCATGGCCAGCAATCGCGCGGC
AAGGTCGAGCGGCTGCTCGAGGCGCTCGGCGTCGAACTGCGCGTGCCGGCCGACAGCCATCTCTGCTGCGGCTCGGCGGG
CACCTATTCGCTGACCCAGCCGGCGCTGTCGTACAAGCTGCGCCGGCAGAAGCTCGCGAAGCTCGGCGAACTCGACGCGC
AGCTGATCGTATCGGGCAACATCGGCTGCATCACGCACCTGCAGAGCGGCACCACCACGCCCGTGATGCACTGGGTCCAG
CTGGTCGAGCAGCTGCTCGACGATTGA

Upstream 100 bases:

>100_bases
CTCGCGGGCCCGCTCGCGGCGATGCACCGCGGCCTGAAGGCCGCATTCGACCCGGACCGCGTGTTCAATCGCGGCCGGCT
CTATCCCGAGCTCTGAGCCC

Downstream 100 bases:

>100_bases
CGCAGTCGCGGCGCGCGCGCCGCCTCGCCGCGTCCGGCCCGCCCGCGGCCGGACGCGCTCCGTATAATGGACCGTGCCGC
GGTCGCGCGGCCCCTTTTCG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRGTPDGDEAESILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQTLEGAPVTRSTQQHLDRCLTCRS
CESTCPSGVQYGRLVEIGRQQVDARVPRPLSQRLVRRALASFVPNAALFAPAMRLGQHLRPLLPRRLRDKVPARTRPLAW
PSARHPRKMLMLAGCVQPAMMPNVNIATARVLDALGIETVIAPEAGCCGAIRLHLNYRDEALADARRNIDAWWPFIEQGA
EAIVMNASGCGATVLEYAHLLRDDAAYADKARRIVELTQDVSGLLAGFAPQLVALARHRARQTVAYHPPCTLQHGQQSRG
KVERLLEALGVELRVPADSHLCCGSAGTYSLTQPALSYKLRRQKLAKLGELDAQLIVSGNIGCITHLQSGTTTPVMHWVQ
LVEQLLDD

Sequences:

>Translated_408_residues
MQTNLADFIRGTPDGDEAESILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQTLEGAPVTRSTQQHLDRCLTCRS
CESTCPSGVQYGRLVEIGRQQVDARVPRPLSQRLVRRALASFVPNAALFAPAMRLGQHLRPLLPRRLRDKVPARTRPLAW
PSARHPRKMLMLAGCVQPAMMPNVNIATARVLDALGIETVIAPEAGCCGAIRLHLNYRDEALADARRNIDAWWPFIEQGA
EAIVMNASGCGATVLEYAHLLRDDAAYADKARRIVELTQDVSGLLAGFAPQLVALARHRARQTVAYHPPCTLQHGQQSRG
KVERLLEALGVELRVPADSHLCCGSAGTYSLTQPALSYKLRRQKLAKLGELDAQLIVSGNIGCITHLQSGTTTPVMHWVQ
LVEQLLDD
>Mature_408_residues
MQTNLADFIRGTPDGDEAESILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQTLEGAPVTRSTQQHLDRCLTCRS
CESTCPSGVQYGRLVEIGRQQVDARVPRPLSQRLVRRALASFVPNAALFAPAMRLGQHLRPLLPRRLRDKVPARTRPLAW
PSARHPRKMLMLAGCVQPAMMPNVNIATARVLDALGIETVIAPEAGCCGAIRLHLNYRDEALADARRNIDAWWPFIEQGA
EAIVMNASGCGATVLEYAHLLRDDAAYADKARRIVELTQDVSGLLAGFAPQLVALARHRARQTVAYHPPCTLQHGQQSRG
KVERLLEALGVELRVPADSHLCCGSAGTYSLTQPALSYKLRRQKLAKLGELDAQLIVSGNIGCITHLQSGTTTPVMHWVQ
LVEQLLDD

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=408, Percent_Identity=55.8823529411765, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788576, Length=414, Percent_Identity=23.9130434782609, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786497, Length=234, Percent_Identity=23.5042735042735, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 44820; Mature: 44820

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRGTPDGDEAESILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQTLE
CCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCC
GAPVTRSTQQHLDRCLTCRSCESTCPSGVQYGRLVEIGRQQVDARVPRPLSQRLVRRALA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SFVPNAALFAPAMRLGQHLRPLLPRRLRDKVPARTRPLAWPSARHPRKMLMLAGCVQPAM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHH
MPNVNIATARVLDALGIETVIAPEAGCCGAIRLHLNYRDEALADARRNIDAWWPFIEQGA
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDAAYADKARRIVELTQDVSGLLAGFAPQLVALARHRA
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQTVAYHPPCTLQHGQQSRGKVERLLEALGVELRVPADSHLCCGSAGTYSLTQPALSYKL
HCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEECCCCCCCCHHHHHHHHHH
RRQKLAKLGELDAQLIVSGNIGCITHLQSGTTTPVMHWVQLVEQLLDD
HHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRGTPDGDEAESILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQTLE
CCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCC
GAPVTRSTQQHLDRCLTCRSCESTCPSGVQYGRLVEIGRQQVDARVPRPLSQRLVRRALA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SFVPNAALFAPAMRLGQHLRPLLPRRLRDKVPARTRPLAWPSARHPRKMLMLAGCVQPAM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHH
MPNVNIATARVLDALGIETVIAPEAGCCGAIRLHLNYRDEALADARRNIDAWWPFIEQGA
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDAAYADKARRIVELTQDVSGLLAGFAPQLVALARHRA
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQTVAYHPPCTLQHGQQSRGKVERLLEALGVELRVPADSHLCCGSAGTYSLTQPALSYKL
HCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEECCCCCCCCHHHHHHHHHH
RRQKLAKLGELDAQLIVSGNIGCITHLQSGTTTPVMHWVQLVEQLLDD
HHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]