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The map label for this gene is dusB [H]

Identifier: 238026250

GI number: 238026250

Start: 611639

End: 612682

Strand: Reverse

Name: dusB [H]

Synonym: bglu_1g05830

Alternate gene names: 238026250

Gene position: 612682-611639 (Counterclockwise)

Preceding gene: 238026251

Following gene: 238026249

Centisome position: 15.68

GC content: 66.67

Gene sequence:

>1044_bases
ATGCCCGTTATCGGCCCCCATATCCTGCGCAACAATCTGTTCGTGGCCCCCATGGCGGGCGTCACGGACCGTCCGTTCCG
TCAGCTCTGCAAGCGGCTCGGCGCGGGTTACGCGGTGTCCGAGATGGTCGCCTCGAACGCGCAGCTGTGGAAAAGCGAGA
AGACCATGCGTCGCGCGAACCACGCCGGCGAGGTCGAGCCGATCGCCGTGCAGATCGCCGGCGCCGATCCGGCGATGATG
GCCGAGGCCGCGCGCTACAACGTGGCGAACGGCGCGCAGATCATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTCTG
CAACGTGGCGGCCGGCTCGGCGCTGCTGCAGAACGAGCCGCTGGTGGTGCGGATCGTCTCGGCCGTGGTGGCGGCGGTGG
GCACCGGCCCCGACGCGGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGAGCACCGCAACGCGCTGACCATC
GCACGTCTGGCCGAGGACGCCGGCATCGCGATGCTGACCGTCCACGGCCGCACGCGCGCCGACCTCTACCGCGGCGACGC
CGAATACGAGACGATCGCGGCCGTGAAGGCGGCGGTAAAGATTCCGGTGGTGGCCAACGGCGACATCACCTCGCCCGCCA
AGGCGAAGGCCGTGCTCGACTTCACCGGTGCCGACGCGCTGATGATCGGTCGCGCCGCGCAAGGTCGCCCATGGCTGTTC
CGCGAAATCGATCATTTCCTGCAAACCGGCACCACGCTGCCGGCGCCGAGCGTCGACGAGATCCAGCAACTGCTCAACGA
GCACCTGGAGGATCATTACGCGTTCTACGGGGAATTTACCGGCGTACGTACTGCACGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGCGCCAACGCGTTCCGGCACCGGATGAACACGCTCGATACCACACGCGAACAGCTTGCCGCCGTCAAC
GAGTATTTCGACGCGCAACGCGCGCTGTCGGACCATCTCGTCTATGTCGGCGACGAAGACGGCACATCCGACCCTATTCA
ATAG

Upstream 100 bases:

>100_bases
GCGGTTCGGCGCTTCGCCATGCCCGGCGGCGCTTACGGTAAAATGCGCGTTTCCCATCTCCCGACCGATGCCCGCGCCCC
GCGCGGGCGCCGCCATTGCG

Downstream 100 bases:

>100_bases
CAGCATGAGCAAGCACAACATCGAACAATGTGTCCGCGAAAGCCTGGACGGTTACTTCCGGGATCTCGACGGCTCCAATC
CGCACGACGTCTACGACATG

Product: putative TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MPVIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVSAVVAAVGTGPDAVPVTLKIRTGWDREHRNALTI
ARLAEDAGIAMLTVHGRTRADLYRGDAEYETIAAVKAAVKIPVVANGDITSPAKAKAVLDFTGADALMIGRAAQGRPWLF
REIDHFLQTGTTLPAPSVDEIQQLLNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDTTREQLAAVN
EYFDAQRALSDHLVYVGDEDGTSDPIQ

Sequences:

>Translated_347_residues
MPVIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVSAVVAAVGTGPDAVPVTLKIRTGWDREHRNALTI
ARLAEDAGIAMLTVHGRTRADLYRGDAEYETIAAVKAAVKIPVVANGDITSPAKAKAVLDFTGADALMIGRAAQGRPWLF
REIDHFLQTGTTLPAPSVDEIQQLLNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDTTREQLAAVN
EYFDAQRALSDHLVYVGDEDGTSDPIQ
>Mature_346_residues
PVIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMMA
EAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVSAVVAAVGTGPDAVPVTLKIRTGWDREHRNALTIA
RLAEDAGIAMLTVHGRTRADLYRGDAEYETIAAVKAAVKIPVVANGDITSPAKAKAVLDFTGADALMIGRAAQGRPWLFR
EIDHFLQTGTTLPAPSVDEIQQLLNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDTTREQLAAVNE
YFDAQRALSDHLVYVGDEDGTSDPIQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.4728033472803, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=33.195020746888, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=30.7017543859649, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=32.618025751073, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=28.4, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=57.9439252336449, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=31.1023622047244, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=27.9835390946502, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=249, Percent_Identity=31.7269076305221, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=29.0983606557377, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=31.4893617021277, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=322, Percent_Identity=26.3975155279503, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=30.9917355371901, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=322, Percent_Identity=23.9130434782609, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585320, Length=287, Percent_Identity=29.2682926829268, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.5652173913043, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=30.1960784313725, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37561; Mature: 37430

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCC
LVVRIVSAVVAAVGTGPDAVPVTLKIRTGWDREHRNALTIARLAEDAGIAMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCHHHHHHHHCCCCEEEEEECCCCHH
DLYRGDAEYETIAAVKAAVKIPVVANGDITSPAKAKAVLDFTGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHH
REIDHFLQTGTTLPAPSVDEIQQLLNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFRHRMNTLDTTREQLAAVNEYFDAQRALSDHLVYVGDEDGTSDPIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
PVIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCC
LVVRIVSAVVAAVGTGPDAVPVTLKIRTGWDREHRNALTIARLAEDAGIAMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCHHHHHHHHCCCCEEEEEECCCCHH
DLYRGDAEYETIAAVKAAVKIPVVANGDITSPAKAKAVLDFTGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHH
REIDHFLQTGTTLPAPSVDEIQQLLNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFRHRMNTLDTTREQLAAVNEYFDAQRALSDHLVYVGDEDGTSDPIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]