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The map label for this gene is speE [H]

Identifier: 238026203

GI number: 238026203

Start: 561900

End: 562757

Strand: Reverse

Name: speE [H]

Synonym: bglu_1g05340

Alternate gene names: 238026203

Gene position: 562757-561900 (Counterclockwise)

Preceding gene: 238026206

Following gene: 238026202

Centisome position: 14.41

GC content: 72.14

Gene sequence:

>858_bases
GTGAACACCACGCTCTGCTTCCAGCCCTGCCCCGACGCGATCTACGGCTTTCCGAACGCCAGCCGCCTCGCCGCGTTCGC
CTCGCCTTACCAGCGCATCGAGGTCTGGGACACGCCGCTGCTCGGCCGCCTGTTTACGCTCGACGGCCGGCCGATGACCT
CGGTCGGCGACGAGGCCGTCTACCACGAGTGCATGACGCATCCGGCTGCGCTCGCGCATCCGCGGCCGCGCCGCGCGCTG
GTGCTAGGCGGCGGCGACGGCGGCGCCGCGCGCCAGCTGCTGAAGCATCCGTCGATCGAACGCATCGTGATCGCCGAACT
CGATGCCGCGGTGGTCGAGATGGCGCGGCGCCACCTCGAGGCCGTGCATCGCGGCGCCTTCGATGATCCGCGTGTCGAGC
TCGTGATCGGCGACGCGGCGGATTACGTCGACCACACGCCGCTGCGCTTCGATCTGGTCGTGTTCGACCTCACGCCGCCC
GATTCGCCGGCCGCCGGCCTCTACACGCGCGCGTTCCTCGCCCGGCTCAAGCGGCGCCTCACGCCCTGCGCGGCGCTCTC
GATGCATCTGGGCGCGCCGGCCTTCCACGCGCCGCGCATCGCCGGGCTCGTCGCCGAACTGCGCGCCTCGTTCGCCGTGG
TCGCGCCGCTCGCCGCGCCGGTGCCGCTCTACGGCGGCGAATGGCTGATGGCGGTGGCGAGCGACACGCTCGATGCGGGC
GCGCTGTTCGCGCACGATATCGAGCAACGGCTCGCCGCGCGCGGCATCGCCGATCTGCGGTTCTACGACGCCGGACTCCA
TCCGGCGCTGTTCACCCTGCGCCGCGCGCTGCGCGATACACTCGGCATGCGCCACTGA

Upstream 100 bases:

>100_bases
CGCATCGGCCGGGCCCGGCCGCCGGCATCGCCCAGCGCCTTCTATAATTCGGGGCAGCCCCGCCCTCGCCCGCCAACCCG
CCGCCCGCCTGCCCGCCGCC

Downstream 100 bases:

>100_bases
CGCTGACGCGGCGCCCCGTCCCACCATCCCGCAGGAGATCGTTCAATGACCGGTCCGCGTCCGGCCGACGTGCCTTGGTT
GACGCCTTATCTGACCGTCC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNTTLCFQPCPDAIYGFPNASRLAAFASPYQRIEVWDTPLLGRLFTLDGRPMTSVGDEAVYHECMTHPAALAHPRPRRAL
VLGGGDGGAARQLLKHPSIERIVIAELDAAVVEMARRHLEAVHRGAFDDPRVELVIGDAADYVDHTPLRFDLVVFDLTPP
DSPAAGLYTRAFLARLKRRLTPCAALSMHLGAPAFHAPRIAGLVAELRASFAVVAPLAAPVPLYGGEWLMAVASDTLDAG
ALFAHDIEQRLAARGIADLRFYDAGLHPALFTLRRALRDTLGMRH

Sequences:

>Translated_285_residues
MNTTLCFQPCPDAIYGFPNASRLAAFASPYQRIEVWDTPLLGRLFTLDGRPMTSVGDEAVYHECMTHPAALAHPRPRRAL
VLGGGDGGAARQLLKHPSIERIVIAELDAAVVEMARRHLEAVHRGAFDDPRVELVIGDAADYVDHTPLRFDLVVFDLTPP
DSPAAGLYTRAFLARLKRRLTPCAALSMHLGAPAFHAPRIAGLVAELRASFAVVAPLAAPVPLYGGEWLMAVASDTLDAG
ALFAHDIEQRLAARGIADLRFYDAGLHPALFTLRRALRDTLGMRH
>Mature_285_residues
MNTTLCFQPCPDAIYGFPNASRLAAFASPYQRIEVWDTPLLGRLFTLDGRPMTSVGDEAVYHECMTHPAALAHPRPRRAL
VLGGGDGGAARQLLKHPSIERIVIAELDAAVVEMARRHLEAVHRGAFDDPRVELVIGDAADYVDHTPLRFDLVVFDLTPP
DSPAAGLYTRAFLARLKRRLTPCAALSMHLGAPAFHAPRIAGLVAELRASFAVVAPLAAPVPLYGGEWLMAVASDTLDAG
ALFAHDIEQRLAARGIADLRFYDAGLHPALFTLRRALRDTLGMRH

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=260, Percent_Identity=28.0769230769231, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786312, Length=255, Percent_Identity=33.7254901960784, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25154106, Length=261, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645616, Length=215, Percent_Identity=29.7674418604651, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323175, Length=254, Percent_Identity=28.3464566929134, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325326, Length=254, Percent_Identity=26.7716535433071, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24645443, Length=253, Percent_Identity=30.8300395256917, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355941, Length=209, Percent_Identity=31.1004784688995, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 30978; Mature: 30978

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTLCFQPCPDAIYGFPNASRLAAFASPYQRIEVWDTPLLGRLFTLDGRPMTSVGDEAV
CCCCEECCCCCHHHHCCCCHHHHHHHHCCHHHEEECCCCHHCEEEEECCCCCCCCCHHHH
YHECMTHPAALAHPRPRRALVLGGGDGGAARQLLKHPSIERIVIAELDAAVVEMARRHLE
HHHHHCCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHH
AVHRGAFDDPRVELVIGDAADYVDHTPLRFDLVVFDLTPPDSPAAGLYTRAFLARLKRRL
HHHCCCCCCCCEEEEECCCHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
TPCAALSMHLGAPAFHAPRIAGLVAELRASFAVVAPLAAPVPLYGGEWLMAVASDTLDAG
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCHH
ALFAHDIEQRLAARGIADLRFYDAGLHPALFTLRRALRDTLGMRH
HHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTTLCFQPCPDAIYGFPNASRLAAFASPYQRIEVWDTPLLGRLFTLDGRPMTSVGDEAV
CCCCEECCCCCHHHHCCCCHHHHHHHHCCHHHEEECCCCHHCEEEEECCCCCCCCCHHHH
YHECMTHPAALAHPRPRRALVLGGGDGGAARQLLKHPSIERIVIAELDAAVVEMARRHLE
HHHHHCCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHH
AVHRGAFDDPRVELVIGDAADYVDHTPLRFDLVVFDLTPPDSPAAGLYTRAFLARLKRRL
HHHCCCCCCCCEEEEECCCHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
TPCAALSMHLGAPAFHAPRIAGLVAELRASFAVVAPLAAPVPLYGGEWLMAVASDTLDAG
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCHH
ALFAHDIEQRLAARGIADLRFYDAGLHPALFTLRRALRDTLGMRH
HHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]