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The map label for this gene is phoB [C]

Identifier: 238025565

GI number: 238025565

Start: 2805551

End: 2806240

Strand: Reverse

Name: phoB [C]

Synonym: bglu_2g22620

Alternate gene names: 238025565

Gene position: 2806240-2805551 (Counterclockwise)

Preceding gene: 238025568

Following gene: 238025556

Centisome position: 99.25

GC content: 67.68

Gene sequence:

>690_bases
ATGCTGACCGCCGTGCTGGAAGACGATGCCGACCAACGCGACCTGATCATGTTGTGGCTCGAACATGGCGGGCATCGCGT
ACACGGTTTTGCGACGGGCGCCGCGTTTACCGAGGCGCTGAAGAACGAGCGATTCCAGTTGCTCGTGATCGACTGGATGC
TGCCCGACACCACGGGCGACGCGATGATCCAGTGGGTGCGCGACCACCTGGGCTGGAACGTGCCCGTGATCGTGCTGACC
GCGCGCGACGACGAGCAGACGGTGCTGACCGCGCTGCGCGCCGGCGCCGACGACTATCTGGTGAAGCCGGCCCGCGCGGC
CGAAATGCTGGCGCGGATCGCGGCGGTGGCGCGCCGCTACGGGATCGGCGGCAAGGCGCCGCTCACGCTCGGCGCCTACG
AGATCGACATCCAGCGCGTGCGGATCGCCGTGAACGGCAGCTCGGTCGACCTCACGCAGAAGGAGTTCGACCTGGCCGTC
TACATGTTCCAGAGCCCGGGCCAGCTGCTGTCGCGCGACCACCTGCTGAACCGCGTCTGGGGCGTGCATGCCGACGTCGA
CACGCGCACCGTCGACACGCACGTGAGCCGGCTGCGCAAGAAGCTGCAGCTGGACGGTTCCAACGGCTGGAAGGTCATCC
CGATCTACGGCTACGGCTACCGCTGCGTGCGGCTCGACTCGCCCGAGTAA

Upstream 100 bases:

>100_bases
CCGGCCGCGACGCGCGCGGCGAGCCGCGCCGGTCATAAGCGATTTCCCAATCGCCCGGCGGGTGAGTAAGATGGGCCATT
TTCTGGAGGGAGTATCCGCA

Downstream 100 bases:

>100_bases
GGCGCGAGGGGCGGCCCGGCGCGGCGGCATGCAGCGGTGCGCGCGGCCGGAGCGGGCCTGGCGCTCAGCGCCACGGGCCC
GGCGGCGATTCGCGGCCGCC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLTAVLEDDADQRDLIMLWLEHGGHRVHGFATGAAFTEALKNERFQLLVIDWMLPDTTGDAMIQWVRDHLGWNVPVIVLT
ARDDEQTVLTALRAGADDYLVKPARAAEMLARIAAVARRYGIGGKAPLTLGAYEIDIQRVRIAVNGSSVDLTQKEFDLAV
YMFQSPGQLLSRDHLLNRVWGVHADVDTRTVDTHVSRLRKKLQLDGSNGWKVIPIYGYGYRCVRLDSPE

Sequences:

>Translated_229_residues
MLTAVLEDDADQRDLIMLWLEHGGHRVHGFATGAAFTEALKNERFQLLVIDWMLPDTTGDAMIQWVRDHLGWNVPVIVLT
ARDDEQTVLTALRAGADDYLVKPARAAEMLARIAAVARRYGIGGKAPLTLGAYEIDIQRVRIAVNGSSVDLTQKEFDLAV
YMFQSPGQLLSRDHLLNRVWGVHADVDTRTVDTHVSRLRKKLQLDGSNGWKVIPIYGYGYRCVRLDSPE
>Mature_229_residues
MLTAVLEDDADQRDLIMLWLEHGGHRVHGFATGAAFTEALKNERFQLLVIDWMLPDTTGDAMIQWVRDHLGWNVPVIVLT
ARDDEQTVLTALRAGADDYLVKPARAAEMLARIAAVARRYGIGGKAPLTLGAYEIDIQRVRIAVNGSSVDLTQKEFDLAV
YMFQSPGQLLSRDHLLNRVWGVHADVDTRTVDTHVSRLRKKLQLDGSNGWKVIPIYGYGYRCVRLDSPE

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=32.8767123287671, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789809, Length=223, Percent_Identity=30.0448430493274, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=29.3333333333333, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=33.1838565022422, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790860, Length=220, Percent_Identity=30.4545454545455, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=28.6995515695067, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=31.5555555555556, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787375, Length=204, Percent_Identity=29.9019607843137, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=28.125, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786784, Length=182, Percent_Identity=32.4175824175824, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=31.3636363636364, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=27.8761061946903, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI2367329, Length=176, Percent_Identity=34.0909090909091, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693140, Length=228, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25679; Mature: 25679

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAVLEDDADQRDLIMLWLEHGGHRVHGFATGAAFTEALKNERFQLLVIDWMLPDTTGD
CEEEEECCCCCCCCEEEEEEECCCEEEEEEECHHHHHHHHHCCCEEEEEEEEECCCCCHH
AMIQWVRDHLGWNVPVIVLTARDDEQTVLTALRAGADDYLVKPARAAEMLARIAAVARRY
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHH
GIGGKAPLTLGAYEIDIQRVRIAVNGSSVDLTQKEFDLAVYMFQSPGQLLSRDHLLNRVW
CCCCCCCEEEEEEEEEEEEEEEEEECCCEECCHHHCEEEEEEECCCCHHHHHHHHHHHHH
GVHADVDTRTVDTHVSRLRKKLQLDGSNGWKVIPIYGYGYRCVRLDSPE
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCC
>Mature Secondary Structure
MLTAVLEDDADQRDLIMLWLEHGGHRVHGFATGAAFTEALKNERFQLLVIDWMLPDTTGD
CEEEEECCCCCCCCEEEEEEECCCEEEEEEECHHHHHHHHHCCCEEEEEEEEECCCCCHH
AMIQWVRDHLGWNVPVIVLTARDDEQTVLTALRAGADDYLVKPARAAEMLARIAAVARRY
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHH
GIGGKAPLTLGAYEIDIQRVRIAVNGSSVDLTQKEFDLAVYMFQSPGQLLSRDHLLNRVW
CCCCCCCEEEEEEEEEEEEEEEEEECCCEECCHHHCEEEEEEECCCCHHHHHHHHHHHHH
GVHADVDTRTVDTHVSRLRKKLQLDGSNGWKVIPIYGYGYRCVRLDSPE
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]