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The map label for this gene is mdeA [H]

Identifier: 238025282

GI number: 238025282

Start: 2430234

End: 2431526

Strand: Direct

Name: mdeA [H]

Synonym: bglu_2g19560

Alternate gene names: 238025282

Gene position: 2430234-2431526 (Clockwise)

Preceding gene: 238025280

Following gene: 238025284

Centisome position: 85.95

GC content: 67.05

Gene sequence:

>1293_bases
ATGGCGTATTCCAGCTATCACAAGAAGGATTTGAACGGTCGCCCGCTGCACCCCGAAACGCAGATGATGTCCTACGGCTA
CGATCCGTTCCTCTCCGAGGGCGCGGTCAAGCCGCCGGTGTTCCTCACCTCCACCTTCGCGTTCAAGTCGGCCGAGGACG
GCGCCGAGTTCTTCGACATCGTGTCGGGCCGCAAGCCGCTGCCCGAGGGCGAGGCGGCCGGCCTGGTCTACAGCCGCTTC
AATCACCCGAACCTGGAAATCGTCGAGGACCGGCTCGCGCTGCTCGACGGCTCGGAGGCGGCGGTCGTCACCTCCAGCGG
CATGTCGGCGATCTCCACGGTGTTCCTCGCATTCCTGCGCCCCGGCGACCAGCTCGTCCAGTCGGTGCCGCTCTACGGCG
GCACCGAGACGCTGATCTCGAAGATCTTCCCCGACTGGGGGATCGGCTCGCACGCGATCCAGAAAGGCCTCGCGCCGGCC
TCGATCCGCGACGCGCTCGAGGCGGCCGCGCAAAAGGGCCCGGTGCGTCTCGTCTACGTCGAGACGCCGGCGAACCCCAC
CAATTCGCTGATCGACCTCGAAGGCATGGTGGCCGAAATCGATGCGTTCGAGCAGCGCCACGGCTATCGTCCGATCTCGG
TCTGCGACAACACGCTGCTCGGGCCGATCTTCCAGAAGCCGGCCGCGCACGGTGTCGACCTGTCGATCTATTCGCTGACG
AAATACGTGGGCGGCCACAGCGACCTGGTGGCGGGCGGCGTGACGGGCAGGCGCGCGCTGATGACGAAGGTGCGCTCGAT
TCGCAGCGCGTTCGGCTCGCAGCTCGATCCGCATTCGTCGTGGATGCTGCTGCGCTCGATGGAAACGGTCGTGCTGCGCA
TGACCCAGGCCACGCGCACGGCCAGCCAGGTGGCACGCTGGCTAGCCGGCAACCCGCACCAGAAGGTGCAGGTGTTCCAT
CCCGAATGCATCGCCGACGACGCGTATCAGGCCGTGTACCGGCGCCATTGCACGGGGGCCGGCTCGACCTTCGCGTTCGT
GCTGAACGGCGGGCGCGCGCAGGCGTTTCGCTTCATCAACGCCTTGCACCTGTTCAAGTCGGCGGTCAGCCTGGGCGGCT
CGGAATCGCTGATCTGCCATCCCGCCTCGACCACCCACTCGGGCGTATCGGCCGCGCAGCGCGAGCGGGACGGCGTATCG
GAAGGGCTGATCCGCGTCTCGATCGGCCTCGAACACGGCGACGACCTGATCGCCGATCTCGACCAGGCGTTCCAGGCCTG
CGCGAAGGGCTGA

Upstream 100 bases:

>100_bases
GCAACTCTACACTGACTGGCGATGCTGCCGTCGCACGAAGCCGCCGCGCATCCGCGCGCGCGTGCCGGCGCTCCCATCAT
TCAAGGTCGAGGAGACAAAC

Downstream 100 bases:

>100_bases
AGCCGGCACGGCGCTGACCGCCTGCCGCCACGACAGGCAGGGCGCGAGACCTCGCGTGGTGCCGCCGGGCCCGCCGTCGG
GCCGCCCGTCCTCCTCCACA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAYSSYHKKDLNGRPLHPETQMMSYGYDPFLSEGAVKPPVFLTSTFAFKSAEDGAEFFDIVSGRKPLPEGEAAGLVYSRF
NHPNLEIVEDRLALLDGSEAAVVTSSGMSAISTVFLAFLRPGDQLVQSVPLYGGTETLISKIFPDWGIGSHAIQKGLAPA
SIRDALEAAAQKGPVRLVYVETPANPTNSLIDLEGMVAEIDAFEQRHGYRPISVCDNTLLGPIFQKPAAHGVDLSIYSLT
KYVGGHSDLVAGGVTGRRALMTKVRSIRSAFGSQLDPHSSWMLLRSMETVVLRMTQATRTASQVARWLAGNPHQKVQVFH
PECIADDAYQAVYRRHCTGAGSTFAFVLNGGRAQAFRFINALHLFKSAVSLGGSESLICHPASTTHSGVSAAQRERDGVS
EGLIRVSIGLEHGDDLIADLDQAFQACAKG

Sequences:

>Translated_430_residues
MAYSSYHKKDLNGRPLHPETQMMSYGYDPFLSEGAVKPPVFLTSTFAFKSAEDGAEFFDIVSGRKPLPEGEAAGLVYSRF
NHPNLEIVEDRLALLDGSEAAVVTSSGMSAISTVFLAFLRPGDQLVQSVPLYGGTETLISKIFPDWGIGSHAIQKGLAPA
SIRDALEAAAQKGPVRLVYVETPANPTNSLIDLEGMVAEIDAFEQRHGYRPISVCDNTLLGPIFQKPAAHGVDLSIYSLT
KYVGGHSDLVAGGVTGRRALMTKVRSIRSAFGSQLDPHSSWMLLRSMETVVLRMTQATRTASQVARWLAGNPHQKVQVFH
PECIADDAYQAVYRRHCTGAGSTFAFVLNGGRAQAFRFINALHLFKSAVSLGGSESLICHPASTTHSGVSAAQRERDGVS
EGLIRVSIGLEHGDDLIADLDQAFQACAKG
>Mature_429_residues
AYSSYHKKDLNGRPLHPETQMMSYGYDPFLSEGAVKPPVFLTSTFAFKSAEDGAEFFDIVSGRKPLPEGEAAGLVYSRFN
HPNLEIVEDRLALLDGSEAAVVTSSGMSAISTVFLAFLRPGDQLVQSVPLYGGTETLISKIFPDWGIGSHAIQKGLAPAS
IRDALEAAAQKGPVRLVYVETPANPTNSLIDLEGMVAEIDAFEQRHGYRPISVCDNTLLGPIFQKPAAHGVDLSIYSLTK
YVGGHSDLVAGGVTGRRALMTKVRSIRSAFGSQLDPHSSWMLLRSMETVVLRMTQATRTASQVARWLAGNPHQKVQVFHP
ECIADDAYQAVYRRHCTGAGSTFAFVLNGGRAQAFRFINALHLFKSAVSLGGSESLICHPASTTHSGVSAAQRERDGVSE
GLIRVSIGLEHGDDLIADLDQAFQACAKG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=412, Percent_Identity=34.2233009708738, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI299473759, Length=412, Percent_Identity=31.5533980582524, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25453487, Length=412, Percent_Identity=29.126213592233, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1790375, Length=398, Percent_Identity=35.929648241206, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789383, Length=409, Percent_Identity=27.8728606356968, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=30.1204819277108, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=30.690537084399, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=31.2849162011173, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319307, Length=398, Percent_Identity=31.6582914572864, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=25.858123569794, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=28.25, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=26.685393258427, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=23.8805970149254, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922554, Length=400, Percent_Identity=33, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46308; Mature: 46177

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSSYHKKDLNGRPLHPETQMMSYGYDPFLSEGAVKPPVFLTSTFAFKSAEDGAEFFDI
CCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCEEEEEHHHHCCCCCHHHHHHH
VSGRKPLPEGEAAGLVYSRFNHPNLEIVEDRLALLDGSEAAVVTSSGMSAISTVFLAFLR
HCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
PGDQLVQSVPLYGGTETLISKIFPDWGIGSHAIQKGLAPASIRDALEAAAQKGPVRLVYV
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEE
ETPANPTNSLIDLEGMVAEIDAFEQRHGYRPISVCDNTLLGPIFQKPAAHGVDLSIYSLT
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHH
KYVGGHSDLVAGGVTGRRALMTKVRSIRSAFGSQLDPHSSWMLLRSMETVVLRMTQATRT
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASQVARWLAGNPHQKVQVFHPECIADDAYQAVYRRHCTGAGSTFAFVLNGGRAQAFRFIN
HHHHHHHHCCCCCCEEEEECCHHHCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHH
ALHLFKSAVSLGGSESLICHPASTTHSGVSAAQRERDGVSEGLIRVSIGLEHGDDLIADL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHCEEEEEEECCCCHHHHHHH
DQAFQACAKG
HHHHHHHHCC
>Mature Secondary Structure 
AYSSYHKKDLNGRPLHPETQMMSYGYDPFLSEGAVKPPVFLTSTFAFKSAEDGAEFFDI
CCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCEEEEEHHHHCCCCCHHHHHHH
VSGRKPLPEGEAAGLVYSRFNHPNLEIVEDRLALLDGSEAAVVTSSGMSAISTVFLAFLR
HCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
PGDQLVQSVPLYGGTETLISKIFPDWGIGSHAIQKGLAPASIRDALEAAAQKGPVRLVYV
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEE
ETPANPTNSLIDLEGMVAEIDAFEQRHGYRPISVCDNTLLGPIFQKPAAHGVDLSIYSLT
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHH
KYVGGHSDLVAGGVTGRRALMTKVRSIRSAFGSQLDPHSSWMLLRSMETVVLRMTQATRT
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASQVARWLAGNPHQKVQVFHPECIADDAYQAVYRRHCTGAGSTFAFVLNGGRAQAFRFIN
HHHHHHHHCCCCCCEEEEECCHHHCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHH
ALHLFKSAVSLGGSESLICHPASTTHSGVSAAQRERDGVSEGLIRVSIGLEHGDDLIADL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHCEEEEEEECCCCHHHHHHH
DQAFQACAKG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]