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The map label for this gene is leuA2 [H]

Identifier: 238025270

GI number: 238025270

Start: 2415501

End: 2416871

Strand: Direct

Name: leuA2 [H]

Synonym: bglu_2g19420

Alternate gene names: 238025270

Gene position: 2415501-2416871 (Clockwise)

Preceding gene: 238025269

Following gene: 238025271

Centisome position: 85.43

GC content: 65.21

Gene sequence:

>1371_bases
ATGCAAGCCAACATCATCGACTGCTCGCTGCGCGAAGGATGCCAGACCATCCAGTGCAATTTCGATATCGAGCAAAGCGA
GATTCTCGCCAGGAAGATCGCCGAGTTTGGCGTCGACATGATCGAATGCGGCCACCCCAAGATTTCGCCGTATGAACTCG
AGCGCGTGCGTGCCGTGGTGCGCGTCTCGCCGCTGCCGGTGCTGGCGCATGCGCGCTGCCGTAGCGAGGACATCGACGCG
GTGGCCGAGTCCGGCGCCGCCTGGGTGGGCATGTTCGCCTCGTTCAATCCGGTATCGCTGGCCACGAAATTCGGCGGCAT
GGGCAAGGATCAGGTGCTGGCGCTATTCGAGGCGTCGATTCGCTACGCCAAGTCCCGCGGCCTGAGCGTGCGCGCCACCA
TCGAGGACGCCGGCCGGACCGAGCAGTCCGATCTGTTCCGTATCTATCAGACCGCGGTGGCCGCCGGCGCCGACCGCATC
TGCTTCGCCGACAGCGTCGGCGCGCTGCTTCCGTCCGAGACCTACGACATCCTGGCGCTGCTGCGCCATGAGTTTCGCCA
GGTCCAGTTCGAATATCACGTCCACAACGATCGCGGCCTGGCCCTGGCCAGTTCGCTGGAGGCGCTGCGCGCCGGTGTCG
AGTGGATTTCCACGAGTTGCAACGGCATCGGGGAGCGCGCCGGGGTGACCGACACGTTTCAGTTGGCCGCCTTGCTGGAG
AGCCGCTTCGGCGACGCTTCGCGCCGGCTCGACAAGGCGCGCCAGCTCTCGGAACTGGTCAGGGTCTTCAGCCGCATCGA
CGGCTCGCCGATGCAGCCGATCGTCGGCGACAACGTCTTCGCCCATGCCTCGCGGCTGCATCGCATCGCCTGCGAGAAGG
ATGCGTCGGCCTACAACATCCATTCCCCGGCCCTGGCCGGCGACGCGGAGGTCCGCGAAAAGCACCCGCCGATGCACGAG
CAAGACCTGTTTCTCGTGCCCTTCGAGAAATCGTCCACCGAGCTTCGCTACCACCGCCACGGCCCCGGCAAGCGTTTCGT
GATGCTCGACCACCGCCTGCTGAGCGCCTCGCCGTTCTATTTCATCGCGCGCAAGGTCGAGGTGATCGCGGAAAACGAGG
AGGGCCACGTGGATACCCACCGCCACAACTGCGACTCCGTTTTCATGTTCCTCGGCGACGACGAGGACTATCGCGGGCTG
ACGGTCGAAGTCAGCATTGCCGGCGTATCGCGGACCCTGACCAGCCCGGCAACCGCCTTCGTGCCGGCAGGCGCGGAGCA
TTCCTACCGCTTCGTGGCCGGCAAGGGCTCGTTCATCAACTTCGTCCACAAGGGCGAATACGCGCGGAGCCTGCTCGAAA
TCGCCGCCTGA

Upstream 100 bases:

>100_bases
AGCCATGCCGGCACCGGCGGCTCCGCTCTCGCCCCGCGAGCGCCGCCGGCGCCGGCCACCCGATGACCGCCCCGTTGCGA
TACTCCTCCAGGACATGCCC

Downstream 100 bases:

>100_bases
GCGGCCCATGCCGTGACCGCTGCCGGGCCTCTCCGAGGCCCAGGCCAGGCGATGCGCGATGGCCGCCGGCACGGCCTCAT
CTCGATTCGACAACCCATCT

Product: Isopropylmalate/homocitrate/citramalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQANIIDCSLREGCQTIQCNFDIEQSEILARKIAEFGVDMIECGHPKISPYELERVRAVVRVSPLPVLAHARCRSEDIDA
VAESGAAWVGMFASFNPVSLATKFGGMGKDQVLALFEASIRYAKSRGLSVRATIEDAGRTEQSDLFRIYQTAVAAGADRI
CFADSVGALLPSETYDILALLRHEFRQVQFEYHVHNDRGLALASSLEALRAGVEWISTSCNGIGERAGVTDTFQLAALLE
SRFGDASRRLDKARQLSELVRVFSRIDGSPMQPIVGDNVFAHASRLHRIACEKDASAYNIHSPALAGDAEVREKHPPMHE
QDLFLVPFEKSSTELRYHRHGPGKRFVMLDHRLLSASPFYFIARKVEVIAENEEGHVDTHRHNCDSVFMFLGDDEDYRGL
TVEVSIAGVSRTLTSPATAFVPAGAEHSYRFVAGKGSFINFVHKGEYARSLLEIAA

Sequences:

>Translated_456_residues
MQANIIDCSLREGCQTIQCNFDIEQSEILARKIAEFGVDMIECGHPKISPYELERVRAVVRVSPLPVLAHARCRSEDIDA
VAESGAAWVGMFASFNPVSLATKFGGMGKDQVLALFEASIRYAKSRGLSVRATIEDAGRTEQSDLFRIYQTAVAAGADRI
CFADSVGALLPSETYDILALLRHEFRQVQFEYHVHNDRGLALASSLEALRAGVEWISTSCNGIGERAGVTDTFQLAALLE
SRFGDASRRLDKARQLSELVRVFSRIDGSPMQPIVGDNVFAHASRLHRIACEKDASAYNIHSPALAGDAEVREKHPPMHE
QDLFLVPFEKSSTELRYHRHGPGKRFVMLDHRLLSASPFYFIARKVEVIAENEEGHVDTHRHNCDSVFMFLGDDEDYRGL
TVEVSIAGVSRTLTSPATAFVPAGAEHSYRFVAGKGSFINFVHKGEYARSLLEIAA
>Mature_456_residues
MQANIIDCSLREGCQTIQCNFDIEQSEILARKIAEFGVDMIECGHPKISPYELERVRAVVRVSPLPVLAHARCRSEDIDA
VAESGAAWVGMFASFNPVSLATKFGGMGKDQVLALFEASIRYAKSRGLSVRATIEDAGRTEQSDLFRIYQTAVAAGADRI
CFADSVGALLPSETYDILALLRHEFRQVQFEYHVHNDRGLALASSLEALRAGVEWISTSCNGIGERAGVTDTFQLAALLE
SRFGDASRRLDKARQLSELVRVFSRIDGSPMQPIVGDNVFAHASRLHRIACEKDASAYNIHSPALAGDAEVREKHPPMHE
QDLFLVPFEKSSTELRYHRHGPGKRFVMLDHRLLSASPFYFIARKVEVIAENEEGHVDTHRHNCDSVFMFLGDDEDYRGL
TVEVSIAGVSRTLTSPATAFVPAGAEHSYRFVAGKGSFINFVHKGEYARSLLEIAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=323, Percent_Identity=30.9597523219814, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=305, Percent_Identity=31.4754098360656, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=305, Percent_Identity=30.8196721311475, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887 [H]

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 50403; Mature: 50403

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANIIDCSLREGCQTIQCNFDIEQSEILARKIAEFGVDMIECGHPKISPYELERVRAVV
CCCCEEECHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHH
RVSPLPVLAHARCRSEDIDAVAESGAAWVGMFASFNPVSLATKFGGMGKDQVLALFEASI
HCCCCCHHHHHHCCCCHHHHHHHCCCEEEEHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
RYAKSRGLSVRATIEDAGRTEQSDLFRIYQTAVAAGADRICFADSVGALLPSETYDILAL
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHH
LRHEFRQVQFEYHVHNDRGLALASSLEALRAGVEWISTSCNGIGERAGVTDTFQLAALLE
HHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SRFGDASRRLDKARQLSELVRVFSRIDGSPMQPIVGDNVFAHASRLHRIACEKDASAYNI
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
HSPALAGDAEVREKHPPMHEQDLFLVPFEKSSTELRYHRHGPGKRFVMLDHRLLSASPFY
CCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECHHHCCCCHH
FIARKVEVIAENEEGHVDTHRHNCDSVFMFLGDDEDYRGLTVEVSIAGVSRTLTSPATAF
HHEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHCCCCCEE
VPAGAEHSYRFVAGKGSFINFVHKGEYARSLLEIAA
EECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MQANIIDCSLREGCQTIQCNFDIEQSEILARKIAEFGVDMIECGHPKISPYELERVRAVV
CCCCEEECHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHH
RVSPLPVLAHARCRSEDIDAVAESGAAWVGMFASFNPVSLATKFGGMGKDQVLALFEASI
HCCCCCHHHHHHCCCCHHHHHHHCCCEEEEHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
RYAKSRGLSVRATIEDAGRTEQSDLFRIYQTAVAAGADRICFADSVGALLPSETYDILAL
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHH
LRHEFRQVQFEYHVHNDRGLALASSLEALRAGVEWISTSCNGIGERAGVTDTFQLAALLE
HHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SRFGDASRRLDKARQLSELVRVFSRIDGSPMQPIVGDNVFAHASRLHRIACEKDASAYNI
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
HSPALAGDAEVREKHPPMHEQDLFLVPFEKSSTELRYHRHGPGKRFVMLDHRLLSASPFY
CCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECHHHCCCCHH
FIARKVEVIAENEEGHVDTHRHNCDSVFMFLGDDEDYRGLTVEVSIAGVSRTLTSPATAF
HHEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHCCCCCEE
VPAGAEHSYRFVAGKGSFINFVHKGEYARSLLEIAA
EECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]