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The map label for this gene is otsA [H]

Identifier: 238025174

GI number: 238025174

Start: 2300286

End: 2301707

Strand: Direct

Name: otsA [H]

Synonym: bglu_2g18450

Alternate gene names: 238025174

Gene position: 2300286-2301707 (Clockwise)

Preceding gene: 238025171

Following gene: 238025176

Centisome position: 81.36

GC content: 68.28

Gene sequence:

>1422_bases
GTGAGCCGTCTCGTCGTGGTATCGAATCGCACCTATGATCCGGACAGCCCGACGCCGGGCGGCCTGGCCGTCGCGCTGAA
CGAGAGTCTGCAGCAGCGCGGCGGCATCTGGTTCGGCTGGAGCGGCAAGCTCACCGAAGCCGACCCGGCTACCCAGCCAG
TGCAGACGCGCGAAGTGGGCAACATGCGGCTCGCCACCATCGACATGTCGCAGCGCGACTACGACGCGTTCTATCTCGGC
TACTCCAACAACGTGCTCTGGCCGGTGTTCCACTACCGGCTCGACCTCGCCAACTTCGACGTGCAGTTCAGCGACGGCTA
TCGCCGCGTGAACCGGCTGTTCGCGCAAAAGCTGCTGCCGCTGCTCGAGCCCGACGACGTGCTCTGGGTCCACGACTATC
ACCTGATCCCGCTCGCCACCGAACTGCGCGCCCAGGGCTGCCGCAACCGCATCGGCTTCTTCCTGCACATCCCCGTGCCG
CCGCCGCAGATCATGGCCGCCATCCCCGAACACGAATGGCTGATGCGCTCGCTGTTCGCCTACGACCTGGTCGGCTTCCA
GGCGCATACCGACGTCACCCACTTCGTGCGCTACGCCGAGGCCGAGGCCGAGGCGCAACTGATCGACGGCGAGCGGCTGC
GCGCCTTCGGCCGCACCATCCGCGTCGGCGCGTTCCCGATCGGCATCGACGTGGACGGCTTCTCGCAAATGGCCACCGAC
GACGACGGCCTCAACGTCTACGAACAGATGCGCGACGAATACTCGCGCCGTAAGCTGCTGCTCGGCGTGGACCGGCTCGA
TTACTCGAAAGGGCTGCCGCAGCGCGTGCAGGCGTTCCGCGCCATGCTCGAGATGTTCCCGGAGATGCGCAAGCACGCCA
CGCTGATCCAGATCGCCGCGCCCAGCCGCGAGGACGTGAGCGCCTACAGCCGGCTGCGCCAGCAGATGGATGCGTTGTGC
GGCTCGATCAACGGCGACTACGGCGAACTCGACTGGATGCCGGTACGCTACATCCACCGCAGCCTGGACCGCACCTCGCT
GCCGGGCCTCTATCGCGCGAGCCGCGTGGCGCTCGTCACGCCGCTGCGCGACGGCATGAACCTGGTGGCCAAGGAATTCA
TCGCCGCGCAGGACGCGAAGAACCCGGGCGTGCTGGTGCTCTCGCGCTTCGCCGGCGCCGCCGAGCAGCTGACCGACGCG
CTGCTCGTGAATCCCTACGATATCCAGGGCACCGCGCGCGCGATCCACGCCGCGCTGACGATGCCGCTGGAGGAACGCGT
GCGGCGCCATGCGGCGCTGCTGGCCCAGATCCGCAGACACGACGTGCACCACTGGGCGGCCGGCTTTCTCGGCGCGCTCG
ACGGCACGAATCGCCCGGCGCAGGCGCGGCGCACGCGCGAACTGGCCGATTCCGTCGCCTAG

Upstream 100 bases:

>100_bases
GTGGCGTTCGACGGCGTCATCGCCGGCTTGCCGGGCCTGCCGGAGACCGGCGGCCACCATCAAAGATCATCGGAGTCCCA
ACGAACCGCAGGAGCCCATC

Downstream 100 bases:

>100_bases
TTCCGCGGCGGCGGCACGCCCGCCTCGGCCGCCGCCGCCGCGCCCGCCCCGCCTCAGCCGCCGCTACGCCGCGGCCTGCT
AATCGGCCGTGCGGCTCTGC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRLVVVSNRTYDPDSPTPGGLAVALNESLQQRGGIWFGWSGKLTEADPATQPVQTREVGNMRLATIDMSQRDYDAFYLG
YSNNVLWPVFHYRLDLANFDVQFSDGYRRVNRLFAQKLLPLLEPDDVLWVHDYHLIPLATELRAQGCRNRIGFFLHIPVP
PPQIMAAIPEHEWLMRSLFAYDLVGFQAHTDVTHFVRYAEAEAEAQLIDGERLRAFGRTIRVGAFPIGIDVDGFSQMATD
DDGLNVYEQMRDEYSRRKLLLGVDRLDYSKGLPQRVQAFRAMLEMFPEMRKHATLIQIAAPSREDVSAYSRLRQQMDALC
GSINGDYGELDWMPVRYIHRSLDRTSLPGLYRASRVALVTPLRDGMNLVAKEFIAAQDAKNPGVLVLSRFAGAAEQLTDA
LLVNPYDIQGTARAIHAALTMPLEERVRRHAALLAQIRRHDVHHWAAGFLGALDGTNRPAQARRTRELADSVA

Sequences:

>Translated_473_residues
MSRLVVVSNRTYDPDSPTPGGLAVALNESLQQRGGIWFGWSGKLTEADPATQPVQTREVGNMRLATIDMSQRDYDAFYLG
YSNNVLWPVFHYRLDLANFDVQFSDGYRRVNRLFAQKLLPLLEPDDVLWVHDYHLIPLATELRAQGCRNRIGFFLHIPVP
PPQIMAAIPEHEWLMRSLFAYDLVGFQAHTDVTHFVRYAEAEAEAQLIDGERLRAFGRTIRVGAFPIGIDVDGFSQMATD
DDGLNVYEQMRDEYSRRKLLLGVDRLDYSKGLPQRVQAFRAMLEMFPEMRKHATLIQIAAPSREDVSAYSRLRQQMDALC
GSINGDYGELDWMPVRYIHRSLDRTSLPGLYRASRVALVTPLRDGMNLVAKEFIAAQDAKNPGVLVLSRFAGAAEQLTDA
LLVNPYDIQGTARAIHAALTMPLEERVRRHAALLAQIRRHDVHHWAAGFLGALDGTNRPAQARRTRELADSVA
>Mature_472_residues
SRLVVVSNRTYDPDSPTPGGLAVALNESLQQRGGIWFGWSGKLTEADPATQPVQTREVGNMRLATIDMSQRDYDAFYLGY
SNNVLWPVFHYRLDLANFDVQFSDGYRRVNRLFAQKLLPLLEPDDVLWVHDYHLIPLATELRAQGCRNRIGFFLHIPVPP
PQIMAAIPEHEWLMRSLFAYDLVGFQAHTDVTHFVRYAEAEAEAQLIDGERLRAFGRTIRVGAFPIGIDVDGFSQMATDD
DGLNVYEQMRDEYSRRKLLLGVDRLDYSKGLPQRVQAFRAMLEMFPEMRKHATLIQIAAPSREDVSAYSRLRQQMDALCG
SINGDYGELDWMPVRYIHRSLDRTSLPGLYRASRVALVTPLRDGMNLVAKEFIAAQDAKNPGVLVLSRFAGAAEQLTDAL
LVNPYDIQGTARAIHAALTMPLEERVRRHAALLAQIRRHDVHHWAAGFLGALDGTNRPAQARRTRELADSVA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=455, Percent_Identity=47.4725274725275, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71987012, Length=430, Percent_Identity=28.3720930232558, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI115533558, Length=427, Percent_Identity=29.5081967213115, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115533560, Length=427, Percent_Identity=29.5081967213115, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=468, Percent_Identity=34.8290598290598, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6320279, Length=403, Percent_Identity=29.0322580645161, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323537, Length=411, Percent_Identity=28.9537712895377, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323917, Length=393, Percent_Identity=28.498727735369, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=37.037037037037, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53426; Mature: 53294

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRTYDPDSPTPGGLAVALNESLQQRGGIWFGWSGKLTEADPATQPVQTREVG
CCCEEEEECCCCCCCCCCCCCEEEEECHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCC
NMRLATIDMSQRDYDAFYLGYSNNVLWPVFHYRLDLANFDVQFSDGYRRVNRLFAQKLLP
CEEEEEEECCCCCCCEEEEECCCCEEEEEHHEEEEHCCCEEEECHHHHHHHHHHHHHHHH
LLEPDDVLWVHDYHLIPLATELRAQGCRNRIGFFLHIPVPPPQIMAAIPEHEWLMRSLFA
CCCCCCEEEEECEEEECHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHHHH
YDLVGFQAHTDVTHFVRYAEAEAEAQLIDGERLRAFGRTIRVGAFPIGIDVDGFSQMATD
HHHHCCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEEEECCCCCHHHCCC
DDGLNVYEQMRDEYSRRKLLLGVDRLDYSKGLPQRVQAFRAMLEMFPEMRKHATLIQIAA
CCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEC
PSREDVSAYSRLRQQMDALCGSINGDYGELDWMPVRYIHRSLDRTSLPGLYRASRVALVT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCEEEEE
PLRDGMNLVAKEFIAAQDAKNPGVLVLSRFAGAAEQLTDALLVNPYDIQGTARAIHAALT
CCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHC
MPLEERVRRHAALLAQIRRHDVHHWAAGFLGALDGTNRPAQARRTRELADSVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLVVVSNRTYDPDSPTPGGLAVALNESLQQRGGIWFGWSGKLTEADPATQPVQTREVG
CCEEEEECCCCCCCCCCCCCEEEEECHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCC
NMRLATIDMSQRDYDAFYLGYSNNVLWPVFHYRLDLANFDVQFSDGYRRVNRLFAQKLLP
CEEEEEEECCCCCCCEEEEECCCCEEEEEHHEEEEHCCCEEEECHHHHHHHHHHHHHHHH
LLEPDDVLWVHDYHLIPLATELRAQGCRNRIGFFLHIPVPPPQIMAAIPEHEWLMRSLFA
CCCCCCEEEEECEEEECHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHHHH
YDLVGFQAHTDVTHFVRYAEAEAEAQLIDGERLRAFGRTIRVGAFPIGIDVDGFSQMATD
HHHHCCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEEEECCCCCHHHCCC
DDGLNVYEQMRDEYSRRKLLLGVDRLDYSKGLPQRVQAFRAMLEMFPEMRKHATLIQIAA
CCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEC
PSREDVSAYSRLRQQMDALCGSINGDYGELDWMPVRYIHRSLDRTSLPGLYRASRVALVT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCEEEEE
PLRDGMNLVAKEFIAAQDAKNPGVLVLSRFAGAAEQLTDALLVNPYDIQGTARAIHAALT
CCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHC
MPLEERVRRHAALLAQIRRHDVHHWAAGFLGALDGTNRPAQARRTRELADSVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA