Click here to switch to the map view.

The map label for this gene is hsrA [H]

Identifier: 238025137

GI number: 238025137

Start: 2251916

End: 2253313

Strand: Direct

Name: hsrA [H]

Synonym: bglu_2g18020

Alternate gene names: 238025137

Gene position: 2251916-2253313 (Clockwise)

Preceding gene: 238025135

Following gene: 238025138

Centisome position: 79.65

GC content: 68.96

Gene sequence:

>1398_bases
ATGCCGCCCGTCGTCCATCCCTTCATCGCACCGCTGATCGTCGCCTGCGCGTTGTTCATGGAGACGGTGGACGCCAACAT
CATCGTCACCGCCTTGCCCGCGATGGCCCGGGCCTTCGGCCACAGCCCCGTCACGCTCAACATCGCGATCACCGCCTACG
TCGTGGGGCTCGGCGTGTTCATCCCGATCTGCGGCTGGCTGGCCGACCGCTACGGCGCGCGCACCGTGTTCCGCACCGCG
ATCGGCATCTTCGTGGCCGGCTCGCTGATGTGCGCGGCCTCCACCAACCTCGCGCTGTTCACGTTCGCGCGCTTCGTGCA
GGGCGTGGGCGGCGCGATGATGGTGCCGGTCGGGCGCATCATCATTTTTCGCGCGGTGCCGCGCTCGGACCTGATCCGCG
CGATGAACTACCTGAGCCTGCCCGCCCTCTTCGGCCCCGCGTTCGGGCCGCTGCTCGGCGGCTTCCTCACCACCTACCTG
CACTGGCGCCTGATCTTCTTCATCAACGTGCCGGTGGGCATCTACGGCATCTATCTCGCCAACAAGTACATCGCCAACAC
GCACGAGCCGAACCCGGGGCCGCTCGACTGGTTCGGCTTCCTGCTGTCGGCCGCGGGCGCCTCGCTGCTGCTGATGGGGC
TCACCATGCTGGACGGCGCGCTCGTCAGCCAGCGCGCCGCGTTCCTGATGTGCGTGGCGGGCGGCCTGCTGCTGAGCGGC
TACGTGCTCTATGCGCGGCGCGCCGCGCAGCCGGTGCTCGACCTGCGGTTCCTGAAGATTCCCACCTTCCATGCGAGCGT
GGCGGGCGGCTCGCTGTTTCGCATCGGACTCGGCGCAGTGCCGTTCCTGCTGCCGCTGACGCTGCAGGAGGGGCTCGGCA
TGAGCGCGTTCACCTCCGGGCTCGTGACCTGCGCATCGGCGGTGGGCGGCACGCTCTCGCGGCTGCTGTCGCAGAAGCTG
CTGCGCCGCTACGGCTTTCGCCGCGTGCTGATGGTGAACGCCGCGCTCGCCGGCCTTGCCATCGCGATGTACGGCACCTT
CTATCCGGGCATGCCGACCTGGGCGATCTGGGTAATCGTGCTGGTGGGCGGCGTGTTCCCCGCGCTGCAGTTCACCAGCC
TCAACTCGGTGATCTACGCCGAGATCGCGCCGCGCGACATCGGCCGCGCCACCAGCCTCGGCAGCGTGGTCCAGCAGATG
TCGCTGGGCCTCGGCGTGACGGTGGCCGGCATCGTGCTGCATCTGTCGGTGGCGCTGCGCGGCCATCCCTCGGTGCAGTG
GTCCGATTTCTGGCCGGCATTCCTGGTGGTGGGGCTCTGCTCGTTCGCATCGATTCCAATCACGCGCCGGCTCGCGCCGA
ATGCGGGCGACGAGATCGCGCGCGGCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCCTGGCCGTTCGTCCAGGTCACTGGCGGCGGCGGTCGCGCCGACGCCGGGGCGGGATCGATGCAATAATGGCGCC
TTTGCCGCGCGTCGATCGCC

Downstream 100 bases:

>100_bases
CGCGCGACGCCGGCCCGCCGCGCCGGCGAGGCACGGGTGAGAGGCGAAACGGGAAAACGGCGCAAAGCCGGCCGGAAGAA
CGCCGTGGCGGCCGCGCCGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPPVVHPFIAPLIVACALFMETVDANIIVTALPAMARAFGHSPVTLNIAITAYVVGLGVFIPICGWLADRYGARTVFRTA
IGIFVAGSLMCAASTNLALFTFARFVQGVGGAMMVPVGRIIIFRAVPRSDLIRAMNYLSLPALFGPAFGPLLGGFLTTYL
HWRLIFFINVPVGIYGIYLANKYIANTHEPNPGPLDWFGFLLSAAGASLLLMGLTMLDGALVSQRAAFLMCVAGGLLLSG
YVLYARRAAQPVLDLRFLKIPTFHASVAGGSLFRIGLGAVPFLLPLTLQEGLGMSAFTSGLVTCASAVGGTLSRLLSQKL
LRRYGFRRVLMVNAALAGLAIAMYGTFYPGMPTWAIWVIVLVGGVFPALQFTSLNSVIYAEIAPRDIGRATSLGSVVQQM
SLGLGVTVAGIVLHLSVALRGHPSVQWSDFWPAFLVVGLCSFASIPITRRLAPNAGDEIARGKRG

Sequences:

>Translated_465_residues
MPPVVHPFIAPLIVACALFMETVDANIIVTALPAMARAFGHSPVTLNIAITAYVVGLGVFIPICGWLADRYGARTVFRTA
IGIFVAGSLMCAASTNLALFTFARFVQGVGGAMMVPVGRIIIFRAVPRSDLIRAMNYLSLPALFGPAFGPLLGGFLTTYL
HWRLIFFINVPVGIYGIYLANKYIANTHEPNPGPLDWFGFLLSAAGASLLLMGLTMLDGALVSQRAAFLMCVAGGLLLSG
YVLYARRAAQPVLDLRFLKIPTFHASVAGGSLFRIGLGAVPFLLPLTLQEGLGMSAFTSGLVTCASAVGGTLSRLLSQKL
LRRYGFRRVLMVNAALAGLAIAMYGTFYPGMPTWAIWVIVLVGGVFPALQFTSLNSVIYAEIAPRDIGRATSLGSVVQQM
SLGLGVTVAGIVLHLSVALRGHPSVQWSDFWPAFLVVGLCSFASIPITRRLAPNAGDEIARGKRG
>Mature_464_residues
PPVVHPFIAPLIVACALFMETVDANIIVTALPAMARAFGHSPVTLNIAITAYVVGLGVFIPICGWLADRYGARTVFRTAI
GIFVAGSLMCAASTNLALFTFARFVQGVGGAMMVPVGRIIIFRAVPRSDLIRAMNYLSLPALFGPAFGPLLGGFLTTYLH
WRLIFFINVPVGIYGIYLANKYIANTHEPNPGPLDWFGFLLSAAGASLLLMGLTMLDGALVSQRAAFLMCVAGGLLLSGY
VLYARRAAQPVLDLRFLKIPTFHASVAGGSLFRIGLGAVPFLLPLTLQEGLGMSAFTSGLVTCASAVGGTLSRLLSQKLL
RRYGFRRVLMVNAALAGLAIAMYGTFYPGMPTWAIWVIVLVGGVFPALQFTSLNSVIYAEIAPRDIGRATSLGSVVQQMS
LGLGVTVAGIVLHLSVALRGHPSVQWSDFWPAFLVVGLCSFASIPITRRLAPNAGDEIARGKRG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=454, Percent_Identity=35.4625550660793, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI1788392, Length=468, Percent_Identity=34.1880341880342, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789042, Length=415, Percent_Identity=25.7831325301205, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=26.4058679706601, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87081983, Length=397, Percent_Identity=26.7002518891688, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=227, Percent_Identity=26.8722466960352, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=230, Percent_Identity=29.1304347826087, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49423; Mature: 49291

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVVHPFIAPLIVACALFMETVDANIIVTALPAMARAFGHSPVTLNIAITAYVVGLGVF
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
IPICGWLADRYGARTVFRTAIGIFVAGSLMCAASTNLALFTFARFVQGVGGAMMVPVGRI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEHHHHHHE
IIFRAVPRSDLIRAMNYLSLPALFGPAFGPLLGGFLTTYLHWRLIFFINVPVGIYGIYLA
EEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHH
NKYIANTHEPNPGPLDWFGFLLSAAGASLLLMGLTMLDGALVSQRAAFLMCVAGGLLLSG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVLYARRAAQPVLDLRFLKIPTFHASVAGGSLFRIGLGAVPFLLPLTLQEGLGMSAFTSG
HHHHHHHHCCHHHHHHEEECCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHH
LVTCASAVGGTLSRLLSQKLLRRYGFRRVLMVNAALAGLAIAMYGTFYPGMPTWAIWVIV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVGGVFPALQFTSLNSVIYAEIAPRDIGRATSLGSVVQQMSLGLGVTVAGIVLHLSVALR
HHHHHHHHHHHHCCCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GHPSVQWSDFWPAFLVVGLCSFASIPITRRLAPNAGDEIARGKRG
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
PPVVHPFIAPLIVACALFMETVDANIIVTALPAMARAFGHSPVTLNIAITAYVVGLGVF
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
IPICGWLADRYGARTVFRTAIGIFVAGSLMCAASTNLALFTFARFVQGVGGAMMVPVGRI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEHHHHHHE
IIFRAVPRSDLIRAMNYLSLPALFGPAFGPLLGGFLTTYLHWRLIFFINVPVGIYGIYLA
EEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHH
NKYIANTHEPNPGPLDWFGFLLSAAGASLLLMGLTMLDGALVSQRAAFLMCVAGGLLLSG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVLYARRAAQPVLDLRFLKIPTFHASVAGGSLFRIGLGAVPFLLPLTLQEGLGMSAFTSG
HHHHHHHHCCHHHHHHEEECCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHH
LVTCASAVGGTLSRLLSQKLLRRYGFRRVLMVNAALAGLAIAMYGTFYPGMPTWAIWVIV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVGGVFPALQFTSLNSVIYAEIAPRDIGRATSLGSVVQQMSLGLGVTVAGIVLHLSVALR
HHHHHHHHHHHHCCCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GHPSVQWSDFWPAFLVVGLCSFASIPITRRLAPNAGDEIARGKRG
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]