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The map label for this gene is yiaO [H]

Identifier: 238024740

GI number: 238024740

Start: 1763486

End: 1764478

Strand: Reverse

Name: yiaO [H]

Synonym: bglu_2g13770

Alternate gene names: 238024740

Gene position: 1764478-1763486 (Counterclockwise)

Preceding gene: 238024741

Following gene: 238024739

Centisome position: 62.41

GC content: 63.24

Gene sequence:

>993_bases
ATGAAAACGATCTTCACTCGCACCCGTGTCGCGGTTGCGATCGTGGCCGCGCTGGCCGCGGCCACCACGATGTCGGCGCA
GGCCCGCACGTTCCGCTCGGCCGACGTCCATAGCGACAGCTTCCCGACCAACATGGCCGTCAAGTTCATGAGCGACGAGA
TCAGCAAGGCCACGGGCGGCAAGGATTCGATCAAGGTGTACGGCAACAGCGCGCTCGGCTCGGAAAACGAGACGATCGAC
CAGGTGCGGATCGGCGCGATCGACATGGTTCGCGCCAACGGCGCCGCGTTCAACGAGATCGTGCCGGAATCGCTGATCCC
GTCGTTCCCGTTCCTGTTCCGTGACATCGAGCACTTCCGCAACGTGATGTACGGCCCGGAAGGCCAGAAGATCCTCGACG
CGTTCCAGGCCAAGGGCCTGGTCGCGCTGACCTTCTACGAAAGCGGCGCGCGTTCGATCTACGCGAAGCGTCCGGTCAAG
TCGCCGGCCGACATGAAGGGCCTCAAGGTGCGCGTGCAGCCCTCGGACCTGATGGTCGACGAGATCAAGGCGATGGGCGG
CGTGCCCACCCCGATGCCGTTCTCGGAAGTCTATACCGGCCTGAAGACGGGCCTGGTGGACGCGGCCGAGAACAACCTGC
CGTCGTATGACGAAACCAAGCACTACGAAGTGGCGAACGTGTACTCGGAAACGCAGCACGCCATGACCCCGGAAGTGCTG
GTGTTCTCGAAGAAGATCTGGGACACGCTCTCCCCGCAGGAGCAGACCATCATCAAGAAGGCGGCGGCCGATTCGGTGCC
GTACTACGTGAAGCTCTGGACGGCGCGTGAAAGCGATGCGCGCAAGGCGGTGCTGAAGGGCGGCGCCACCATCGTGCCGG
CCGCGCAGATCGATCGTGCCGCGTTCGTGAAGGCGATGCAGCCGCTCTGGGCCAAGTACGAGAAGACGCCGAAGATGAAG
CAGATCGTCGACGAAATCCAGGCGATCAAGTAA

Upstream 100 bases:

>100_bases
CGCCGCCACGCTCGACACTGCGCGTCCGGCCTCGCGCTGACGCCGTACCGGGGGCGGCGTGCGCCACCCAACACTCGATA
TTCCCAGAGGAGACTTGACC

Downstream 100 bases:

>100_bases
GGCCCGGCATGCGGCCCGGCGATGCCGGCCCGCATGCGCGAACCGGCGGCCGCGCGCGACTGCGCCCGGCCGCCTTTCCT
GTCCGATGAGGATTGAAGCA

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKTIFTRTRVAVAIVAALAAATTMSAQARTFRSADVHSDSFPTNMAVKFMSDEISKATGGKDSIKVYGNSALGSENETID
QVRIGAIDMVRANGAAFNEIVPESLIPSFPFLFRDIEHFRNVMYGPEGQKILDAFQAKGLVALTFYESGARSIYAKRPVK
SPADMKGLKVRVQPSDLMVDEIKAMGGVPTPMPFSEVYTGLKTGLVDAAENNLPSYDETKHYEVANVYSETQHAMTPEVL
VFSKKIWDTLSPQEQTIIKKAAADSVPYYVKLWTARESDARKAVLKGGATIVPAAQIDRAAFVKAMQPLWAKYEKTPKMK
QIVDEIQAIK

Sequences:

>Translated_330_residues
MKTIFTRTRVAVAIVAALAAATTMSAQARTFRSADVHSDSFPTNMAVKFMSDEISKATGGKDSIKVYGNSALGSENETID
QVRIGAIDMVRANGAAFNEIVPESLIPSFPFLFRDIEHFRNVMYGPEGQKILDAFQAKGLVALTFYESGARSIYAKRPVK
SPADMKGLKVRVQPSDLMVDEIKAMGGVPTPMPFSEVYTGLKTGLVDAAENNLPSYDETKHYEVANVYSETQHAMTPEVL
VFSKKIWDTLSPQEQTIIKKAAADSVPYYVKLWTARESDARKAVLKGGATIVPAAQIDRAAFVKAMQPLWAKYEKTPKMK
QIVDEIQAIK
>Mature_330_residues
MKTIFTRTRVAVAIVAALAAATTMSAQARTFRSADVHSDSFPTNMAVKFMSDEISKATGGKDSIKVYGNSALGSENETID
QVRIGAIDMVRANGAAFNEIVPESLIPSFPFLFRDIEHFRNVMYGPEGQKILDAFQAKGLVALTFYESGARSIYAKRPVK
SPADMKGLKVRVQPSDLMVDEIKAMGGVPTPMPFSEVYTGLKTGLVDAAENNLPSYDETKHYEVANVYSETQHAMTPEVL
VFSKKIWDTLSPQEQTIIKKAAADSVPYYVKLWTARESDARKAVLKGGATIVPAAQIDRAAFVKAMQPLWAKYEKTPKMK
QIVDEIQAIK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=301, Percent_Identity=32.890365448505, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36241; Mature: 36241

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIFTRTRVAVAIVAALAAATTMSAQARTFRSADVHSDSFPTNMAVKFMSDEISKATGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KDSIKVYGNSALGSENETIDQVRIGAIDMVRANGAAFNEIVPESLIPSFPFLFRDIEHFR
CCCEEEEECCCCCCCCCCHHHHHHCCEEEEECCCCHHHHHCCHHHCCCCHHHHHHHHHHH
NVMYGPEGQKILDAFQAKGLVALTFYESGARSIYAKRPVKSPADMKGLKVRVQPSDLMVD
HHHCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEECCCHHHHH
EIKAMGGVPTPMPFSEVYTGLKTGLVDAAENNLPSYDETKHYEVANVYSETQHAMTPEVL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
VFSKKIWDTLSPQEQTIIKKAAADSVPYYVKLWTARESDARKAVLKGGATIVPAAQIDRA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEECHHHHHHH
AFVKAMQPLWAKYEKTPKMKQIVDEIQAIK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIFTRTRVAVAIVAALAAATTMSAQARTFRSADVHSDSFPTNMAVKFMSDEISKATGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KDSIKVYGNSALGSENETIDQVRIGAIDMVRANGAAFNEIVPESLIPSFPFLFRDIEHFR
CCCEEEEECCCCCCCCCCHHHHHHCCEEEEECCCCHHHHHCCHHHCCCCHHHHHHHHHHH
NVMYGPEGQKILDAFQAKGLVALTFYESGARSIYAKRPVKSPADMKGLKVRVQPSDLMVD
HHHCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEECCCHHHHH
EIKAMGGVPTPMPFSEVYTGLKTGLVDAAENNLPSYDETKHYEVANVYSETQHAMTPEVL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
VFSKKIWDTLSPQEQTIIKKAAADSVPYYVKLWTARESDARKAVLKGGATIVPAAQIDRA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEECHHHHHHH
AFVKAMQPLWAKYEKTPKMKQIVDEIQAIK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]