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The map label for this gene is paaF [C]

Identifier: 238024522

GI number: 238024522

Start: 1441499

End: 1442296

Strand: Reverse

Name: paaF [C]

Synonym: bglu_2g11280

Alternate gene names: 238024522

Gene position: 1442296-1441499 (Counterclockwise)

Preceding gene: 238024523

Following gene: 238024521

Centisome position: 51.01

GC content: 75.69

Gene sequence:

>798_bases
ATGCGGTACGAGACCAGATACGAGACGATCCGCGTCACCGAGGAGCAGCGCATCGCCACGGTCACGCTCGCGCGGCCCGA
GGTGCGCAACGCGTTCAACGAGACCATGATCGCGGAGCTGACGGCCGCGTTCGGCGAGCTCGACGCGCACGACGGCATCC
GCGCGGTGGTGCTGGCCGCCGAGGGGCCGGCGTTCTGCGCCGGCGCGGACCTGAGCTGGATGCGCCGCATGGCCGGCTAT
AGCGACGACGAGAACCGCGCCGACGCGCGCCGGCTCGCGCGCATGCTGGAGGCGGTGTACCGCTGCGGCAAGCCGGTGAT
CGCGCGCGTGCACGGCGACGCCTACGCGGGCGGGGTCGGCCTGGTGGCGGCCTGCGACGTCGCGATCGGCGCCGAGCCGG
CGCGCTTCTGCCTCTCGGAGGCGCGGCTCGGGCTGATTCCGGCCACCATCGCGCCCTACGTGGTGCGTGCGCTCGGCGAG
CGCGCCGCGCGCCGCTACTTCACCACCGCCGAGGTGTTCGGCAGCGCGCGCGCCGCCCAGCTCGGGCTGATCCACGAGGC
GGTGCCGGCCGAGGTGCTCGACGCGGCCGTCGCGCAGCTGGGCGACGCGCTGCGCGCCAACGGGCCGGGCGCCGTGCGGG
CCGCCAAGCGGCTGGTGGCCGAGGTGGCCGGCCGCGCGCTCGACGACGCGCTGATCGAGCAGACCGCCGACTGGATCGCG
GCGACGCGCGCGGGCGCCGAGGCGCGCGAGGGCATCGCCGCGTTCCTCGACAAGCGCACGCCGTCATGGCGCGAATGA

Upstream 100 bases:

>100_bases
GCGCGCACGCGCGACGTGCTCGGGCTGGGCCTGGCCGCCGCCATGAACGCGCCGATCGAGCAGACGCGCTTCGGCGTGTT
CCGGATGTAAGGAGGCAGGC

Downstream 100 bases:

>100_bases
GGCCGCTGCGCTGGCGGCCCGCCGGCGCGGGGCGCTAGCCCGCATCGCGTCCCTGATTCGCCGTCCCGGCCTTTCCGAAC
CCCGCTTACCGAATCCCGAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRYETRYETIRVTEEQRIATVTLARPEVRNAFNETMIAELTAAFGELDAHDGIRAVVLAAEGPAFCAGADLSWMRRMAGY
SDDENRADARRLARMLEAVYRCGKPVIARVHGDAYAGGVGLVAACDVAIGAEPARFCLSEARLGLIPATIAPYVVRALGE
RAARRYFTTAEVFGSARAAQLGLIHEAVPAEVLDAAVAQLGDALRANGPGAVRAAKRLVAEVAGRALDDALIEQTADWIA
ATRAGAEAREGIAAFLDKRTPSWRE

Sequences:

>Translated_265_residues
MRYETRYETIRVTEEQRIATVTLARPEVRNAFNETMIAELTAAFGELDAHDGIRAVVLAAEGPAFCAGADLSWMRRMAGY
SDDENRADARRLARMLEAVYRCGKPVIARVHGDAYAGGVGLVAACDVAIGAEPARFCLSEARLGLIPATIAPYVVRALGE
RAARRYFTTAEVFGSARAAQLGLIHEAVPAEVLDAAVAQLGDALRANGPGAVRAAKRLVAEVAGRALDDALIEQTADWIA
ATRAGAEAREGIAAFLDKRTPSWRE
>Mature_265_residues
MRYETRYETIRVTEEQRIATVTLARPEVRNAFNETMIAELTAAFGELDAHDGIRAVVLAAEGPAFCAGADLSWMRRMAGY
SDDENRADARRLARMLEAVYRCGKPVIARVHGDAYAGGVGLVAACDVAIGAEPARFCLSEARLGLIPATIAPYVVRALGE
RAARRYFTTAEVFGSARAAQLGLIHEAVPAEVLDAAVAQLGDALRANGPGAVRAAKRLVAEVAGRALDDALIEQTADWIA
ATRAGAEAREGIAAFLDKRTPSWRE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=27.34375, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=273, Percent_Identity=30.03663003663, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4502327, Length=277, Percent_Identity=28.158844765343, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI62530384, Length=178, Percent_Identity=27.5280898876405, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=31.0077519379845, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788597, Length=268, Percent_Identity=29.8507462686567, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=29.3436293436293, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=23.5074626865672, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=26.4925373134328, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788682, Length=217, Percent_Identity=27.6497695852535, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.9212598425197, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=177, Percent_Identity=29.9435028248588, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=282, Percent_Identity=24.822695035461, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=152, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=25.1207729468599, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=33.2046332046332, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=29.5546558704453, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=29.5546558704453, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.3243243243243, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583165, Length=212, Percent_Identity=25.9433962264151, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921018, Length=212, Percent_Identity=27.8301886792453, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654903, Length=202, Percent_Identity=26.2376237623762, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=29.2035398230088, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28382; Mature: 28382

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYETRYETIRVTEEQRIATVTLARPEVRNAFNETMIAELTAAFGELDAHDGIRAVVLAA
CCCCCHHHEEEECCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
EGPAFCAGADLSWMRRMAGYSDDENRADARRLARMLEAVYRCGKPVIARVHGDAYAGGVG
CCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCHH
LVAACDVAIGAEPARFCLSEARLGLIPATIAPYVVRALGERAARRYFTTAEVFGSARAAQ
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LGLIHEAVPAEVLDAAVAQLGDALRANGPGAVRAAKRLVAEVAGRALDDALIEQTADWIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRAGAEAREGIAAFLDKRTPSWRE
HHHCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRYETRYETIRVTEEQRIATVTLARPEVRNAFNETMIAELTAAFGELDAHDGIRAVVLAA
CCCCCHHHEEEECCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
EGPAFCAGADLSWMRRMAGYSDDENRADARRLARMLEAVYRCGKPVIARVHGDAYAGGVG
CCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCHH
LVAACDVAIGAEPARFCLSEARLGLIPATIAPYVVRALGERAARRYFTTAEVFGSARAAQ
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LGLIHEAVPAEVLDAAVAQLGDALRANGPGAVRAAKRLVAEVAGRALDDALIEQTADWIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRAGAEAREGIAAFLDKRTPSWRE
HHHCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA