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The map label for this gene is pmbA [H]

Identifier: 238024469

GI number: 238024469

Start: 1360256

End: 1361614

Strand: Direct

Name: pmbA [H]

Synonym: bglu_2g10650

Alternate gene names: 238024469

Gene position: 1360256-1361614 (Clockwise)

Preceding gene: 238024468

Following gene: 238024470

Centisome position: 48.11

GC content: 71.38

Gene sequence:

>1359_bases
ATGCAATCGAGCCGTATGCAGTGGCAGGAGCTTGCCGCGGAGCTGGAGACTTCGGCCGAGCGCGTGATCGAGCTCGCGCG
GCGGGCCGGCGCGCAGGGCGCCCGGGTCGAGATCGAGGCCGCCGAGTCGAGCGCCGTGACCGTGACGAACGGGGTGAGCA
CGGAGCGCAGCTTTTGCGGCACGCGCGAGATGACGGTCACGGTACTGCGTGACGGCAAACGCGCGCTCGCCAAATCGTCG
GACCTGTCGGACGAGGGGCTCAAGCAAGCGGTCCTCGCGGCCATCGAGGGCACCGCCGCCACCGAGGCGGACCCCGCGGC
CGGCCTGGCCGATCCCGGGCTCCTGGCCCGCGAGTTTCCGGACCTGGATCTGTACCACCCGCTGGATTGGTCGCTCGACG
AGCTCGGCGCCCATGCGCAACGTGCCGAGGACGCGGCACGCGCGCACGATCCGTCGATCAGCACCTCCAAGGGCGTGACG
GTGCGCACGGCGTCGGGCACTTCGCTGCTGGCGACCTCCGCGGGATTTCTCGCGACCGCGCCCTGGTCCGTCCATTCGAT
CGCCTGCGCCCCGGTCGCGTTGCGCGCCGGCGAACGGCAGATCGGCTTCTGGGGCGATGCCAGGCGTGCGTTCGACGATC
TCGCGCCGCCGGAGGCGGTCGGGGCGGCGGCCGCCCGGCGCGCGGTGGGCGCGCTCGGCGCCAGGCAGGTTGCCACGCAG
CAGTGCCCGGTGCTGTTCGAGCCCTCGGCGGCCCTCGGGCTGTTGGGCGATTTGGTGTCGGCGGCCTCGGGCGATGCGCT
CTACCGCAGCGGCTCGTTCCTGAGGAATGGCATCGACGCCCCCCTGTTTCCCTCGCACGTGCAGCTGCGCGAGGAGCCGT
TCCTGAAGCGCGGCATGGCCAGCCGCTGCTTCGACTGCGACGGCATCGCGGGGCAGTCGCGCAGCGTGGTGGAGGAGGGC
TGCCTGCGCGGCTTTTTCCTCGGGCTCTACGCGGCGCGCCGCCTGAACCTGGCGCCCACCGGCAACGGCTACGGGCCGCA
CAACCTCGAGATGCGCAGCACGCACACCCAGGCGGCCGACGACTTCGCCGCGATGCTGGGCAGGCTGCATCGCGGGCTGC
TGGTCACGGAGATGGTCGGCGGCGGAGTGAACCCGTCGACGGGGGATGTCTCGCGGGGCGCCAAGGGGTTCTGGGTGGAG
AACGGCGAGATCCAGTTTTCGGTGAGCGGCATCACGATAGCCTCCAATCTCGGGCGGATGTTCGGCGGGCTGCAGGCGAT
CGGCAGCGACGCGATCACCCGGGGATGGGCGACGTCGGGATCGTGGCTGATCGATGCAATGATGGTCGGGGGGGCTTGA

Upstream 100 bases:

>100_bases
AACGGGGCGGCGCGATCGGCGACATGCTGATTTCGCTGACCCGCCGGTGTGCCTGAGACATGCGATGCACGATGCCGGAT
GGCGAAGGAGGAGCAGGCTG

Downstream 100 bases:

>100_bases
GGCATGAGTGAGGCAAAGCAATCGGGCGCGCGGCTTGCCGCGCGCGACGGGGTGAGTGCGCTGGCGCACGGCATCCTGCT
GGAACAGCATGGCATCGACG

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQSSRMQWQELAAELETSAERVIELARRAGAQGARVEIEAAESSAVTVTNGVSTERSFCGTREMTVTVLRDGKRALAKSS
DLSDEGLKQAVLAAIEGTAATEADPAAGLADPGLLAREFPDLDLYHPLDWSLDELGAHAQRAEDAARAHDPSISTSKGVT
VRTASGTSLLATSAGFLATAPWSVHSIACAPVALRAGERQIGFWGDARRAFDDLAPPEAVGAAAARRAVGALGARQVATQ
QCPVLFEPSAALGLLGDLVSAASGDALYRSGSFLRNGIDAPLFPSHVQLREEPFLKRGMASRCFDCDGIAGQSRSVVEEG
CLRGFFLGLYAARRLNLAPTGNGYGPHNLEMRSTHTQAADDFAAMLGRLHRGLLVTEMVGGGVNPSTGDVSRGAKGFWVE
NGEIQFSVSGITIASNLGRMFGGLQAIGSDAITRGWATSGSWLIDAMMVGGA

Sequences:

>Translated_452_residues
MQSSRMQWQELAAELETSAERVIELARRAGAQGARVEIEAAESSAVTVTNGVSTERSFCGTREMTVTVLRDGKRALAKSS
DLSDEGLKQAVLAAIEGTAATEADPAAGLADPGLLAREFPDLDLYHPLDWSLDELGAHAQRAEDAARAHDPSISTSKGVT
VRTASGTSLLATSAGFLATAPWSVHSIACAPVALRAGERQIGFWGDARRAFDDLAPPEAVGAAAARRAVGALGARQVATQ
QCPVLFEPSAALGLLGDLVSAASGDALYRSGSFLRNGIDAPLFPSHVQLREEPFLKRGMASRCFDCDGIAGQSRSVVEEG
CLRGFFLGLYAARRLNLAPTGNGYGPHNLEMRSTHTQAADDFAAMLGRLHRGLLVTEMVGGGVNPSTGDVSRGAKGFWVE
NGEIQFSVSGITIASNLGRMFGGLQAIGSDAITRGWATSGSWLIDAMMVGGA
>Mature_452_residues
MQSSRMQWQELAAELETSAERVIELARRAGAQGARVEIEAAESSAVTVTNGVSTERSFCGTREMTVTVLRDGKRALAKSS
DLSDEGLKQAVLAAIEGTAATEADPAAGLADPGLLAREFPDLDLYHPLDWSLDELGAHAQRAEDAARAHDPSISTSKGVT
VRTASGTSLLATSAGFLATAPWSVHSIACAPVALRAGERQIGFWGDARRAFDDLAPPEAVGAAAARRAVGALGARQVATQ
QCPVLFEPSAALGLLGDLVSAASGDALYRSGSFLRNGIDAPLFPSHVQLREEPFLKRGMASRCFDCDGIAGQSRSVVEEG
CLRGFFLGLYAARRLNLAPTGNGYGPHNLEMRSTHTQAADDFAAMLGRLHRGLLVTEMVGGGVNPSTGDVSRGAKGFWVE
NGEIQFSVSGITIASNLGRMFGGLQAIGSDAITRGWATSGSWLIDAMMVGGA

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=437, Percent_Identity=36.1556064073227, Blast_Score=261, Evalue=9e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47285; Mature: 47285

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSRMQWQELAAELETSAERVIELARRAGAQGARVEIEAAESSAVTVTNGVSTERSFCG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHCC
TREMTVTVLRDGKRALAKSSDLSDEGLKQAVLAAIEGTAATEADPAAGLADPGLLAREFP
CCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCC
DLDLYHPLDWSLDELGAHAQRAEDAARAHDPSISTSKGVTVRTASGTSLLATSAGFLATA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCEEEC
PWSVHSIACAPVALRAGERQIGFWGDARRAFDDLAPPEAVGAAAARRAVGALGARQVATQ
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QCPVLFEPSAALGLLGDLVSAASGDALYRSGSFLRNGIDAPLFPSHVQLREEPFLKRGMA
CCCEEECCCHHHHHHHHHHHHCCCCHHHHCCHHHHCCCCCCCCCCHHHHHHCHHHHHHHH
SRCFDCDGIAGQSRSVVEEGCLRGFFLGLYAARRLNLAPTGNGYGPHNLEMRSTHTQAAD
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHH
DFAAMLGRLHRGLLVTEMVGGGVNPSTGDVSRGAKGFWVENGEIQFSVSGITIASNLGRM
HHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEEEECCEEHHHHHHHH
FGGLQAIGSDAITRGWATSGSWLIDAMMVGGA
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MQSSRMQWQELAAELETSAERVIELARRAGAQGARVEIEAAESSAVTVTNGVSTERSFCG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHCC
TREMTVTVLRDGKRALAKSSDLSDEGLKQAVLAAIEGTAATEADPAAGLADPGLLAREFP
CCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCC
DLDLYHPLDWSLDELGAHAQRAEDAARAHDPSISTSKGVTVRTASGTSLLATSAGFLATA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCEEEC
PWSVHSIACAPVALRAGERQIGFWGDARRAFDDLAPPEAVGAAAARRAVGALGARQVATQ
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QCPVLFEPSAALGLLGDLVSAASGDALYRSGSFLRNGIDAPLFPSHVQLREEPFLKRGMA
CCCEEECCCHHHHHHHHHHHHCCCCHHHHCCHHHHCCCCCCCCCCHHHHHHCHHHHHHHH
SRCFDCDGIAGQSRSVVEEGCLRGFFLGLYAARRLNLAPTGNGYGPHNLEMRSTHTQAAD
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHH
DFAAMLGRLHRGLLVTEMVGGGVNPSTGDVSRGAKGFWVENGEIQFSVSGITIASNLGRM
HHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEEEECCEEHHHHHHHH
FGGLQAIGSDAITRGWATSGSWLIDAMMVGGA
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]