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The map label for this gene is phzD [H]

Identifier: 238024340

GI number: 238024340

Start: 1148751

End: 1149389

Strand: Direct

Name: phzD [H]

Synonym: bglu_2g09280

Alternate gene names: 238024340

Gene position: 1148751-1149389 (Clockwise)

Preceding gene: 238024339

Following gene: 238024341

Centisome position: 40.63

GC content: 65.57

Gene sequence:

>639_bases
ATGAACACCAGTCGATTCGAAAGCCTCACGGGACGCGTCGATGTCCTGTTCCCCGAATATGACGACCCGCCGTCCGAGCC
GATCACGCTGCTCAAGCGTTGGCTCGCTGCGGCCGACGTCGCGGGCGTCCGCGAGCCCAAGGCGCTCGCGCTCGCGACCG
CCACGTCCGACGGGCGCATGTCTTCGCGCATCATCGCGTTCTCCTCGATCGACGACCGCGGCGTGATTTTCTGCACGCAT
TCGACGAGCCGCAAAGGACGCGAACTCACGGAGACAGGCTGGGCCTCGGGCTTGCTGTATTGGCGTGAAACCGGGCAGCA
GATCATGATTTCCGGCCGGGCGGTTCCGCTCGAGGAAAGCGAGAACGACAAGCTCTGGTTCGGACGCGCGGTGCCGATGC
ACGCGATGTCGAGCGCCTCGCACCAGAGCGACGAACTCGTCGACCGCGAAGCGCTGCTCGCGCACGCCGCCGAGCTGCTC
GCGCTTGGCGTCGCGTTGCCGCGGCCGCCGCGCTTCGTCGGTTATCGGCTCGAGCCTCACGAGATGGAATTCTGGGCCGC
CAGCTCCGACCGGCTTCACCGGCGCCTCAGATACGAACGCAATGGCGACGCTTGGAAAACCATGCAACTCCAACCTTGA

Upstream 100 bases:

>100_bases
ATCGAGGCGGTCGAGGTCAGCGGTCACGGTGTCGTCGTCGCGCGCGGCCGCCTGGGCGTCTAGCGTCGCCCCCGCCTTTT
GCCATCAAGAAAACACTGCC

Downstream 100 bases:

>100_bases
TTCTCTTGCGGATAAGGAACCTGTCTGTGTCTACTTTCGATATTCCGGTTGGCGTCGGCCAAACCGCACTGTTCATTGCC
TGGCAACGTCATGCCGAAAG

Product: Pyridoxamine-phosphate oxidase

Products: Hydrogen peroxide; ammonia; Pyridoxal 5'-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNTSRFESLTGRVDVLFPEYDDPPSEPITLLKRWLAAADVAGVREPKALALATATSDGRMSSRIIAFSSIDDRGVIFCTH
STSRKGRELTETGWASGLLYWRETGQQIMISGRAVPLEESENDKLWFGRAVPMHAMSSASHQSDELVDREALLAHAAELL
ALGVALPRPPRFVGYRLEPHEMEFWAASSDRLHRRLRYERNGDAWKTMQLQP

Sequences:

>Translated_212_residues
MNTSRFESLTGRVDVLFPEYDDPPSEPITLLKRWLAAADVAGVREPKALALATATSDGRMSSRIIAFSSIDDRGVIFCTH
STSRKGRELTETGWASGLLYWRETGQQIMISGRAVPLEESENDKLWFGRAVPMHAMSSASHQSDELVDREALLAHAAELL
ALGVALPRPPRFVGYRLEPHEMEFWAASSDRLHRRLRYERNGDAWKTMQLQP
>Mature_212_residues
MNTSRFESLTGRVDVLFPEYDDPPSEPITLLKRWLAAADVAGVREPKALALATATSDGRMSSRIIAFSSIDDRGVIFCTH
STSRKGRELTETGWASGLLYWRETGQQIMISGRAVPLEESENDKLWFGRAVPMHAMSSASHQSDELVDREALLAHAAELL
ALGVALPRPPRFVGYRLEPHEMEFWAASSDRLHRRLRYERNGDAWKTMQLQP

Specific function: Involved in the biosynthesis of the antibiotic, phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control. Probably catalyzes the final step in the conversion of trans-2,3- dihydro-3-hyd

COG id: COG0259

COG function: function code H; Pyridoxamine-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxamine 5'-phosphate oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922498, Length=179, Percent_Identity=32.4022346368715, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787926, Length=194, Percent_Identity=31.9587628865979, Blast_Score=101, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17553712, Length=198, Percent_Identity=33.8383838383838, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319509, Length=196, Percent_Identity=30.1020408163265, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644901, Length=179, Percent_Identity=30.7262569832402, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551845, Length=179, Percent_Identity=30.7262569832402, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000659
- InterPro:   IPR019740
- InterPro:   IPR019576
- InterPro:   IPR011576
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF10590 PNPOx_C; PF01243 Pyridox_oxidase [H]

EC number: 1.4.3.5 [C]

Molecular weight: Translated: 23837; Mature: 23837

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSRFESLTGRVDVLFPEYDDPPSEPITLLKRWLAAADVAGVREPKALALATATSDGRM
CCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SSRIIAFSSIDDRGVIFCTHSTSRKGRELTETGWASGLLYWRETGQQIMISGRAVPLEES
CCEEEEEECCCCCEEEEEECCCCCCCCCHHHCCHHHCEEEEECCCCEEEEECCEECCCCC
ENDKLWFGRAVPMHAMSSASHQSDELVDREALLAHAAELLALGVALPRPPRFVGYRLEPH
CCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
EMEFWAASSDRLHRRLRYERNGDAWKTMQLQP
CEEEEECCHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MNTSRFESLTGRVDVLFPEYDDPPSEPITLLKRWLAAADVAGVREPKALALATATSDGRM
CCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SSRIIAFSSIDDRGVIFCTHSTSRKGRELTETGWASGLLYWRETGQQIMISGRAVPLEES
CCEEEEEECCCCCEEEEEECCCCCCCCCHHHCCHHHCEEEEECCCCEEEEECCEECCCCC
ENDKLWFGRAVPMHAMSSASHQSDELVDREALLAHAAELLALGVALPRPPRFVGYRLEPH
CCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
EMEFWAASSDRLHRRLRYERNGDAWKTMQLQP
CEEEEECCHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN [C]

Metal ions: NA

Kcat value (1/min): 1.8 [C]

Specific activity: NA

Km value (mM): 0.002 {Tris,} [C]

Substrates: H2O; O2; Pyridoxamine 5'-phosphate [C]

Specific reaction: H2O + O2 + Pyridoxamine 5'-phosphate --> Hydrogen peroxide + ammonia + Pyridoxal 5'-phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8586283 [H]