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The map label for this gene is fdoH [H]

Identifier: 238024282

GI number: 238024282

Start: 1050956

End: 1051870

Strand: Direct

Name: fdoH [H]

Synonym: bglu_2g08650

Alternate gene names: 238024282

Gene position: 1050956-1051870 (Clockwise)

Preceding gene: 238024281

Following gene: 238024283

Centisome position: 37.17

GC content: 66.12

Gene sequence:

>915_bases
ATGGCATTGCAATCGCTGGACATCAAACGGGTGTCCGCCACCACCACCCCGCCGCCCACCGCGCGCGATCCGGTCACGGG
CACCGTCGCGAAGCTGATCGACGTGTCGAAGTGCATCGGCTGCAAGGCATGCCAGACCGCCTGCATGGAGTGGAACGACC
TGCGCGACGAGATCGGCACCAACGTCGGCGTCTACGACAATCCGGCCGACCTCAGCGAGCATTCGTGGACGGTGATGCGG
TTCTCCGAATACGAGAACCCGGCCGGCGACCTCGAATGGCTGATCCGCAAGGACGGCTGCATGCACTGCGAGGACCCGGG
CTGCCTGAAGGCCTGCCCGTCGCCGGGCGCGATCGTGCAGTACAACAACGGCATCGTCGATTTCCACGAGGAGAACTGCA
TCGGTTGCGGCTATTGCGTGACCGGCTGCCCGTTCAATATCCCGCGGATCTCGAAGCAGGACAACCGTGCCTACAAATGC
ACGCTGTGCTCGGATCGCGTGGCCGTCGGCCAGGAGCCCGCCTGCGTGAAGACCTGCCCGACGGGCGCCATCATGTTCGG
CACCAAGGAGGACATGAAGCACCAGGCGGCCGAACGGATCGCGGACCTGAAGGATCGCGGCTTCGCCCAGGCGGGGCTGT
ACGACCCGCAGGGCGTGGGCGGCACGCACGTGATGTACGTGCTGCACCATGCGGACAAGCCGTCGCTGTACCACGGCCTG
CCCGACAATCCGTCGATCAGCCCGTTCGTGAAGCTCTGGAAGGGCATCGCCAAGCCGCTGGCGGTGGCCGGTCTCGCGCT
GACCGCGCTGGCCGGGTTCTTCCACTACACGCGGGTCGGCCCCAACGAGGTGAGCGAGCAAGAGGACGCCGCCGCCCGTG
AGGAAGCCCGCCGGATCGAGGAGGACACCAAATGA

Upstream 100 bases:

>100_bases
CGCGAACACCCTGACGCCGTCGGTCGGCGACGGCAACTCGCAGACACCGGAATTCAAGTCGTTCCTGGTGAAAGTGGAAA
AGGCATAAGGGGAACCCGAG

Downstream 100 bases:

>100_bases
GCCACCAGGACCCGAACCTGATCGTCCGTTACTCCGCGAATGAACGCTCGAATCACTGGATCACCGCGATCTCGTTCGTG
CTGCTCGCGCTGTCCGGCCT

Product: Fe-S-cluster-containing hydrogenase

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MALQSLDIKRVSATTTPPPTARDPVTGTVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLSEHSWTVMR
FSEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNIPRISKQDNRAYKC
TLCSDRVAVGQEPACVKTCPTGAIMFGTKEDMKHQAAERIADLKDRGFAQAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPFVKLWKGIAKPLAVAGLALTALAGFFHYTRVGPNEVSEQEDAAAREEARRIEEDTK

Sequences:

>Translated_304_residues
MALQSLDIKRVSATTTPPPTARDPVTGTVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLSEHSWTVMR
FSEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNIPRISKQDNRAYKC
TLCSDRVAVGQEPACVKTCPTGAIMFGTKEDMKHQAAERIADLKDRGFAQAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPFVKLWKGIAKPLAVAGLALTALAGFFHYTRVGPNEVSEQEDAAAREEARRIEEDTK
>Mature_303_residues
ALQSLDIKRVSATTTPPPTARDPVTGTVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLSEHSWTVMRF
SEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNIPRISKQDNRAYKCT
LCSDRVAVGQEPACVKTCPTGAIMFGTKEDMKHQAAERIADLKDRGFAQAGLYDPQGVGGTHVMYVLHHADKPSLYHGLP
DNPSISPFVKLWKGIAKPLAVAGLALTALAGFFHYTRVGPNEVSEQEDAAAREEARRIEEDTK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=297, Percent_Identity=70.03367003367, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1787749, Length=292, Percent_Identity=69.1780821917808, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1789370, Length=282, Percent_Identity=30.4964539007092, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=33.6787564766839, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=33.160621761658, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI2367345, Length=168, Percent_Identity=30.3571428571429, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510944, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=29.0502793296089, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082179, Length=174, Percent_Identity=28.1609195402299, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787740, Length=170, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787963, Length=158, Percent_Identity=25.3164556962025, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787478, Length=131, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082180, Length=176, Percent_Identity=26.7045454545455, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367245, Length=172, Percent_Identity=29.0697674418605, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33139; Mature: 33008

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQSLDIKRVSATTTPPPTARDPVTGTVAKLIDVSKCIGCKACQTACMEWNDLRDEIGT
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
NVGVYDNPADLSEHSWTVMRFSEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEENCIGCGYCVTGCPFNIPRISKQDNRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCEEEECC
TGAIMFGTKEDMKHQAAERIADLKDRGFAQAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPFVKLWKGIAKPLAVAGLALTALAGFFHYTRVGPNEVSEQEDAAAREEARRIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EDTK
CCCC
>Mature Secondary Structure 
ALQSLDIKRVSATTTPPPTARDPVTGTVAKLIDVSKCIGCKACQTACMEWNDLRDEIGT
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
NVGVYDNPADLSEHSWTVMRFSEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEENCIGCGYCVTGCPFNIPRISKQDNRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCEEEECC
TGAIMFGTKEDMKHQAAERIADLKDRGFAQAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPFVKLWKGIAKPLAVAGLALTALAGFFHYTRVGPNEVSEQEDAAAREEARRIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EDTK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]