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The map label for this gene is purF [H]

Identifier: 238024266

GI number: 238024266

Start: 1027665

End: 1029197

Strand: Direct

Name: purF [H]

Synonym: bglu_2g08490

Alternate gene names: 238024266

Gene position: 1027665-1029197 (Clockwise)

Preceding gene: 238024265

Following gene: 238024267

Centisome position: 36.35

GC content: 65.69

Gene sequence:

>1533_bases
ATGTGCGGCATCGTAGGCTTTATCTCTCATTCGCCGGTCAACCAGCTCATTTATGACAGCCTGCTGCTGCTGCAGCATCG
CGGTCAGGACGCGGCGGGCATCGTGACGGCGGACGGCAGCAACTTCCACATGTACAAGGCCAACGGCATGGTGCGAGACG
TGTTCCGCACGCGCAACATGCGCAGCCTGCCGGGTACCTCGGGGATCGGCCAGGTCCGCTATCCGACGGCGGGTTCGGCG
TCGAGCGAGGCCGAGGCGCAGCCGTTCTACGTCAACGCGCCGTTCGGCGTCGTGCTCGCCCACAACGGCAACCTGACGAA
CATGGAGCAGCTCAAGGACGAGATGTTCCGCATCGACCGGCGCCACATCAACACGAACTCCGACAGCGAAGTGCTACTGA
ACGTGTTCGCACACGAGCTGCAACTGGCCACCACCGGCCTCGAGCTCGATCCGGCCGCGGTGTTCAAGGCGGTGGCGGGC
GTGCATCGCCGCGCGCAGGGTTCGTATGCGATCGTCTCGCAGATCGCCGGCTACGGCATGCTCGCGTTCCGCGACCCGTT
CGGCATCCGCCCGCTTTGCATCGGCAAGCTCGAGACCGAGCAGGGCACGGAATGGATGGTCGCCTCGGAATCGGTGGCCG
TGGAGGGCATCGGCTTCGAGTTCGTGCGCGACGTCGAGCCGGGCGAGGCGGTGTTCGTCACGCTCGACGGCAGTTTCTAC
ACCCAGCAGTGCGCCGAGAACCCGAGCCTGAACCCGTGCATGTTCGAGTGGGTCTACCTCGCGCGTCCCGATTCGTGCCT
CGACGGCGTGCCCGTCTACAACGTGCGCCTGCGCATGGGCGACTACCTCGCCGAGAAGATCAAGCGCGAGCTCAAGGACG
TGCCGATCGACGTGGTGATGCCGATCCCCGATTCGTCGCGGCCGGCCGCGATGCAGGTGGCCGCCAAGCTCGGCGTCGAG
TATCGCGAGGGCTTCTTCAAGAACCGCTACGTGGGCCGCACCTTCATCATGCCGGGCCAGGCGGTGCGCAAGAAGTCGGT
GCGCCAGAAGCTCAACGCGATGAGCATCGAGTTCAAGGACAAGCACGTGCTGATCGTCGACGACTCGATCGTGCGCGGCA
CGACCTCGCACGAGATCGTGCAGATGGCGCGCGATGCCGGCGCGAAGTCGGTGATCTTCGCCTCGGCCGCGCCGCCCGTG
AAGTTCCCCAACGTCTACGGGATCGACATGCCGACCCGCAACGAGCTCGTCGCGCACGGCCGCACCGACGAGGAAGTGGC
GAAGATGATCGGCGCCGATTACCTGATCTACCAGGATGTGGACGATCTGCGCCGCGCGGTGCGCGACATCAACCCGGCGC
TCCAGGACTTCGAGACCTCGTGCTTCGACGGCCGCTACATCACCGGCCACGTCACGCCCGAATACCTCGATTCGCTCGAG
CGCGCGCGCCTCGCGCCGGCCTCGCAGCTCGATCGCGACCAGAACGGCGAGTCGGGCCGCTCGCAGATGAACCTGCAACT
GTCGGTCGAATGA

Upstream 100 bases:

>100_bases
CGCGAACTGAAGCCGTTCTTGCCCGACGGGCTGGCTGCGTACATCCACATCTGAGTACGCGGCGGCCAAACCGTTTTTGT
ATCCTTGAAGGACCCATGCC

Downstream 100 bases:

>100_bases
AACGCCGCGCCCGTCGTGACACATGTTAGGATGGGCGCTGCGTCGATTTGATCTCAGCTTGCGGACGCGGGGCAGTCACC
CGAAACAGCTAAAGCGAAGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MCGIVGFISHSPVNQLIYDSLLLLQHRGQDAAGIVTADGSNFHMYKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGVVLAHNGNLTNMEQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLATTGLELDPAAVFKAVAG
VHRRAQGSYAIVSQIAGYGMLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVEPGEAVFVTLDGSFY
TQQCAENPSLNPCMFEWVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELKDVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRNELVAHGRTDEEVAKMIGADYLIYQDVDDLRRAVRDINPALQDFETSCFDGRYITGHVTPEYLDSLE
RARLAPASQLDRDQNGESGRSQMNLQLSVE

Sequences:

>Translated_510_residues
MCGIVGFISHSPVNQLIYDSLLLLQHRGQDAAGIVTADGSNFHMYKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGVVLAHNGNLTNMEQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLATTGLELDPAAVFKAVAG
VHRRAQGSYAIVSQIAGYGMLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVEPGEAVFVTLDGSFY
TQQCAENPSLNPCMFEWVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELKDVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRNELVAHGRTDEEVAKMIGADYLIYQDVDDLRRAVRDINPALQDFETSCFDGRYITGHVTPEYLDSLE
RARLAPASQLDRDQNGESGRSQMNLQLSVE
>Mature_510_residues
MCGIVGFISHSPVNQLIYDSLLLLQHRGQDAAGIVTADGSNFHMYKANGMVRDVFRTRNMRSLPGTSGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGVVLAHNGNLTNMEQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLATTGLELDPAAVFKAVAG
VHRRAQGSYAIVSQIAGYGMLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVEPGEAVFVTLDGSFY
TQQCAENPSLNPCMFEWVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELKDVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRNELVAHGRTDEEVAKMIGADYLIYQDVDDLRRAVRDINPALQDFETSCFDGRYITGHVTPEYLDSLE
RARLAPASQLDRDQNGESGRSQMNLQLSVE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.4978902953587, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788651, Length=501, Percent_Identity=58.0838323353293, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1790167, Length=174, Percent_Identity=29.8850574712644, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=487, Percent_Identity=34.7022587268994, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=498, Percent_Identity=50.2008032128514, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=36.6803278688525, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=36.5269461077844, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24659604, Length=486, Percent_Identity=36.8312757201646, Blast_Score=268, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56489; Mature: 56489

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGFISHSPVNQLIYDSLLLLQHRGQDAAGIVTADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHCCC
RSLPGTSGIGQVRYPTAGSASSEAEAQPFYVNAPFGVVLAHNGNLTNMEQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHELQLATTGLELDPAAVFKAVAGVHRRAQGSYAIVSQIAGYGM
HHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCE
LAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVEPGEAVFVTLDGSFY
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHEECCCCCCEEEEEECCCHH
TQQCAENPSLNPCMFEWVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELKDVPIDVVM
HHHHHCCCCCCCHHEEEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRNELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECC
RTDEEVAKMIGADYLIYQDVDDLRRAVRDINPALQDFETSCFDGRYITGHVTPEYLDSLE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEECCCHHHHHHHH
RARLAPASQLDRDQNGESGRSQMNLQLSVE
HHHCCCHHHCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MCGIVGFISHSPVNQLIYDSLLLLQHRGQDAAGIVTADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHCCC
RSLPGTSGIGQVRYPTAGSASSEAEAQPFYVNAPFGVVLAHNGNLTNMEQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHELQLATTGLELDPAAVFKAVAGVHRRAQGSYAIVSQIAGYGM
HHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCE
LAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVEPGEAVFVTLDGSFY
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHEECCCCCCEEEEEECCCHH
TQQCAENPSLNPCMFEWVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELKDVPIDVVM
HHHHHCCCCCCCHHEEEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRNELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECC
RTDEEVAKMIGADYLIYQDVDDLRRAVRDINPALQDFETSCFDGRYITGHVTPEYLDSLE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEECCCHHHHHHHH
RARLAPASQLDRDQNGESGRSQMNLQLSVE
HHHCCCHHHCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10873488; 11248100 [H]