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The map label for this gene is metZ [H]

Identifier: 238024267

GI number: 238024267

Start: 1029444

End: 1030634

Strand: Direct

Name: metZ [H]

Synonym: bglu_2g08500

Alternate gene names: 238024267

Gene position: 1029444-1030634 (Clockwise)

Preceding gene: 238024266

Following gene: 238024270

Centisome position: 36.41

GC content: 67.51

Gene sequence:

>1191_bases
ATGGACGACTCTTTCAATTTCGACACGCTCGCGATTCGCTCGGGCACGCTGCGCAGCGAATACAACGAGCACTCGGAAGC
GATCTTCCTGACCTCGAGCTTCTGCTTCACGAGCGCCGCCGATGCCGCCGAGCGCTTCGCGAATTCCGAGGACAACTACA
CGTATTCGCGCTTCACGAACCCGACCGTCACGATGTTCCAGGACCGGCTCGCGGCGCTGGAGGGCGGCGAGGCCTGCATC
GCGACCGCCTCGGGGATGGCGGCCATCATGTCGGTGGTGATGGCGGCGCTGCAGTCGGGCGACCATCTGGTCAGCTCGCG
CAGCCTGTTCGGCTCGACGCTCGGCATGTTCTCGCAGATCTTCAGCAAGTTCGGCATCACCACCACCTTCGTCGATCCCA
CCGACCTGGAGGCGTGGCGCGCCGCGGTGCGTCCCGAGACGAAGATGTTCTTCCTCGAGACGCCGTCGAACCCGCTCACC
GAGCTGGCCGACATCGAGGCCATCTCGAAGGTCGCGAAGGCCGCCAATGCGCTGTTCGTGGTCGACAACTGCTTCTGCAG
CCCGGCGCTTCAGCAGCCGCTCAAGCTCGGCGCCGACGTGGTGATGCACTCGGCCACCAAGTTTCTCGACGGCCAGGGCC
GCGTGCTGGGCGGCGCGCTGGTGGGCTCGAAGGCGTTCATCACCGGCAAGGTGTTCCCGTTCGTGCGCAGCGCGGGGCCC
ACGCTGTCGGCCTTCAACGCGTGGGTGCTGCTCAAGGGCATGGAAACGCTGTCGCTGCGCGTCGAGAAGCAGTCGGCCAA
CGCGCTGGAGATCGCGCGCTGGCTCGACAGCCATCCGGCCGTCAAGCGCGTGTTCTATCCCGGGCTCGAATCGCATCCGC
AGTACCAGATCGCCAGGCGCCAGCAGAAGGCGGGCGGCGCGGTGGTGTCGTTCGAGCTGAAGGGCGACACGCCGGAGGCG
CAGCGCGCGAATGCGTGGCGCGTGATCGACAGCACCAAGATCGTGTCGATCACCGCGAACCTCGGCGACACGCGCACCAC
GATCACGCATCCGGCCACCACCACGCACTCGCGCATCACGCCCGAGGCGCGCGCCGCGGCGGGCATCAGCGAAGGGCTGA
TCCGGCTCGCGGTGGGGCTGGAGAACGCCGGCGACATCCGCGACGATCTCGCGCGCGGGCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGCCGATGCCGACGCATCGCGGGCTTTTTCGTTGGCCCTCGCGGCCTGGCGTGGCGCGCCCGCGGCGCGGCGCGCCCGAA
TGAAACCGGATGAACCAGAC

Downstream 100 bases:

>100_bases
CGCGCGGGTCGGGCCCGGCGCCGGTCTGGTGCGCCGCCGGGCCGGTGTGGCGTCCGACCGGGACGCGGCGCGGCGTGCCG
CTCGGCGCGCCGCCAGATAC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDDSFNFDTLAIRSGTLRSEYNEHSEAIFLTSSFCFTSAADAAERFANSEDNYTYSRFTNPTVTMFQDRLAALEGGEACI
ATASGMAAIMSVVMAALQSGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLEAWRAAVRPETKMFFLETPSNPLT
ELADIEAISKVAKAANALFVVDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFITGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVKRVFYPGLESHPQYQIARRQQKAGGAVVSFELKGDTPEA
QRANAWRVIDSTKIVSITANLGDTRTTITHPATTTHSRITPEARAAAGISEGLIRLAVGLENAGDIRDDLARGLAG

Sequences:

>Translated_396_residues
MDDSFNFDTLAIRSGTLRSEYNEHSEAIFLTSSFCFTSAADAAERFANSEDNYTYSRFTNPTVTMFQDRLAALEGGEACI
ATASGMAAIMSVVMAALQSGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLEAWRAAVRPETKMFFLETPSNPLT
ELADIEAISKVAKAANALFVVDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFITGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVKRVFYPGLESHPQYQIARRQQKAGGAVVSFELKGDTPEA
QRANAWRVIDSTKIVSITANLGDTRTTITHPATTTHSRITPEARAAAGISEGLIRLAVGLENAGDIRDDLARGLAG
>Mature_396_residues
MDDSFNFDTLAIRSGTLRSEYNEHSEAIFLTSSFCFTSAADAAERFANSEDNYTYSRFTNPTVTMFQDRLAALEGGEACI
ATASGMAAIMSVVMAALQSGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLEAWRAAVRPETKMFFLETPSNPLT
ELADIEAISKVAKAANALFVVDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFITGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVKRVFYPGLESHPQYQIARRQQKAGGAVVSFELKGDTPEA
QRANAWRVIDSTKIVSITANLGDTRTTITHPATTTHSRITPEARAAAGISEGLIRLAVGLENAGDIRDDLARGLAG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=35.3658536585366, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=32.3170731707317, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=29.9694189602446, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=37.8238341968912, Blast_Score=257, Evalue=7e-70,
Organism=Escherichia coli, GI1789383, Length=344, Percent_Identity=27.6162790697674, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=36.4457831325301, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=35.8433734939759, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=33.4231805929919, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=32.9466357308585, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=31.6708229426434, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=28.2926829268293, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321170, Length=292, Percent_Identity=26.7123287671233, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=354, Percent_Identity=26.271186440678, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922554, Length=392, Percent_Identity=34.6938775510204, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42593; Mature: 42593

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSFNFDTLAIRSGTLRSEYNEHSEAIFLTSSFCFTSAADAAERFANSEDNYTYSRFTN
CCCCCCCCEEEEECCCHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECC
PTVTMFQDRLAALEGGEACIATASGMAAIMSVVMAALQSGDHLVSSRSLFGSTLGMFSQI
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FSKFGITTTFVDPTDLEAWRAAVRPETKMFFLETPSNPLTELADIEAISKVAKAANALFV
HHHHCCEEEEECCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEE
VDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFITGKVFPFVRSAGP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCHHHHHHCCCC
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVKRVFYPGLESHPQYQIARR
CHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHH
QQKAGGAVVSFELKGDTPEAQRANAWRVIDSTKIVSITANLGDTRTTITHPATTTHSRIT
HHHCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCC
PEARAAAGISEGLIRLAVGLENAGDIRDDLARGLAG
CHHHHHCCHHHHHHHHEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MDDSFNFDTLAIRSGTLRSEYNEHSEAIFLTSSFCFTSAADAAERFANSEDNYTYSRFTN
CCCCCCCCEEEEECCCHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECC
PTVTMFQDRLAALEGGEACIATASGMAAIMSVVMAALQSGDHLVSSRSLFGSTLGMFSQI
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FSKFGITTTFVDPTDLEAWRAAVRPETKMFFLETPSNPLTELADIEAISKVAKAANALFV
HHHHCCEEEEECCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEE
VDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFITGKVFPFVRSAGP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCHHHHHHCCCC
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVKRVFYPGLESHPQYQIARR
CHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHH
QQKAGGAVVSFELKGDTPEAQRANAWRVIDSTKIVSITANLGDTRTTITHPATTTHSRIT
HHHCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCC
PEARAAAGISEGLIRLAVGLENAGDIRDDLARGLAG
CHHHHHCCHHHHHHHHEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]