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The map label for this gene is leuC [H]

Identifier: 238024251

GI number: 238024251

Start: 1011588

End: 1012997

Strand: Direct

Name: leuC [H]

Synonym: bglu_2g08330

Alternate gene names: 238024251

Gene position: 1011588-1012997 (Clockwise)

Preceding gene: 238024250

Following gene: 238024252

Centisome position: 35.78

GC content: 67.66

Gene sequence:

>1410_bases
ATGGCACAGACTCTCTACGATAAATTGTGGAATTCTCACGTCGTCCACACCGAGGAGGACGGCACCGCGTTGCTCTACAT
CGACCGTCACCTGCTGCACGAAGTGACGAGTCCGCAGGCGTTCGAGGGCCTGAAGCTCGCCGAGCGTCCGGTCTGGCGGA
TCAGCGCGAACCTCGCGGTGTCGGACCACAACGTGCCGACCACCGACCGCACGCACGGCATCGCCGATCCGGTCTCGAAG
CTGCAGGTCGACACGCTCGACGCGAACTGCGACAGCTACGGCATCACCCAGTTCAAGATGAACGACCTGCGCCAGGGCAT
CGTCCACATCATCGGGCCGGAGCAGGGCGCGACGCTGCCGGGCATGACGATCGTCTGCGGGGATTCGCACACCTCCACGC
ACGGCGCGTTCGGCGCGCTCGCGCATGGCATCGGCACCTCGGAGGTCGAGCACGTGCTGGCCACCCAGACGCTGCTGCAG
AAAAAGAGCAAGAACCTGCTGGTGAAAGTGGAAGGCCAGCTGCCGCGCGGCTGCACCGCCAAGGACATCGTGCTCGCGAT
CATCGGCAGGATCGGCACGGCGGGCGGCACCGGCTACGCGATCGAATTCGGCGGCTCGATGATCCGCTCGCTGTCGATGG
AAGGCCGCATGACGGTCTGCAACATGGCGATCGAGGCCGGCGCGCGCGCCGGCATGGTGGCCGTGGACGACACCACCATC
GACTACCTGAAGGGCCGGCCGTTCTCGCCGACCGGGGCCGAATGGGATCAGGCCGTGCAGTACTGGCGCACGTTCGTTTC
GGACCCCGGCGCGCAGTTCGACCGCGTGGTCGAGCTGACGGCCGCCGACATCGTGCCGCAGGTCACCTGGGGCACCTCGC
CGGAGATGGTCACGGCGGTGGACGGCCGCGTGCCCGATCCCGAGCGCGAGAAGGATCCGGTCAAGCGCAACGCGATGGAG
CGCGCGCTTGCCTACATGGCGCTCGAACCGAACACGCCGATCGAATCGATCCAGGTCGACAAGATCTTCATCGGCTCCTG
TACCAACGCCCGCATCGAGGACATCCGCGCCGCCGCCTATGTCGTGAAGAAGCTGAACCGGCGCGTGGCCGCCAACGTGC
GGCTGGCGATGGTGGTGCCGGGCTCGGGCCTCGTGAAGGCGCAGGCCGAGCGCGAGGGGCTCGACAAGGTGTTCCTCGAG
GCCGGCTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGCCTCGCGATGAATGCCGACCGGCTCGATCCGGGCGAGCGCTG
CGCGTCCACCTCGAACCGCAACTTCGAGGGGCGTCAGGGCGCGGGCGGCCGCACCCATCTGGTGAGCCCCGCGATGGCCG
CGGCCGCGGCCATCGAAGGCCATTTCGTCGACATCCGCCGTCTCGGGTGA

Upstream 100 bases:

>100_bases
TCATTGGGTTTTTTTGGAATTTAGGGGTGCCGCGCGGCGGGGTTGATGGCATAATTGCTAGCCAGGAAGCGCCCGTTGTC
ACCCTAACCCCGCATACCCC

Downstream 100 bases:

>100_bases
GCCTCATGTCACGCACGCAGCTGCGCCGCCTCGCGGCGCTCGTCGCCCTCGCGGGCATGGTGCTCGGTCTCGCGGCCTGC
AACACGGTCGCCGGCATGGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRTHGIADPVSK
LQVDTLDANCDSYGITQFKMNDLRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNLLVKVEGQLPRGCTAKDIVLAIIGRIGTAGGTGYAIEFGGSMIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTI
DYLKGRPFSPTGAEWDQAVQYWRTFVSDPGAQFDRVVELTAADIVPQVTWGTSPEMVTAVDGRVPDPEREKDPVKRNAME
RALAYMALEPNTPIESIQVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAANVRLAMVVPGSGLVKAQAEREGLDKVFLE
AGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRRLG

Sequences:

>Translated_469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRTHGIADPVSK
LQVDTLDANCDSYGITQFKMNDLRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNLLVKVEGQLPRGCTAKDIVLAIIGRIGTAGGTGYAIEFGGSMIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTI
DYLKGRPFSPTGAEWDQAVQYWRTFVSDPGAQFDRVVELTAADIVPQVTWGTSPEMVTAVDGRVPDPEREKDPVKRNAME
RALAYMALEPNTPIESIQVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAANVRLAMVVPGSGLVKAQAEREGLDKVFLE
AGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRRLG
>Mature_468_residues
AQTLYDKLWNSHVVHTEEDGTALLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRTHGIADPVSKL
QVDTLDANCDSYGITQFKMNDLRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQK
KSKNLLVKVEGQLPRGCTAKDIVLAIIGRIGTAGGTGYAIEFGGSMIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTID
YLKGRPFSPTGAEWDQAVQYWRTFVSDPGAQFDRVVELTAADIVPQVTWGTSPEMVTAVDGRVPDPEREKDPVKRNAMER
ALAYMALEPNTPIESIQVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAANVRLAMVVPGSGLVKAQAEREGLDKVFLEA
GFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRRLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=29.3956043956044, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI8659555, Length=401, Percent_Identity=27.1820448877805, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI41352693, Length=376, Percent_Identity=26.5957446808511, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=62.8205128205128, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=371, Percent_Identity=27.2237196765499, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=379, Percent_Identity=29.5514511873351, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=379, Percent_Identity=29.5514511873351, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=28.9473684210526, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=26.9135802469136, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.1949152542373, Blast_Score=624, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=30.939226519337, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=28.7323943661972, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=386, Percent_Identity=27.4611398963731, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365315, Length=401, Percent_Identity=27.930174563591, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=401, Percent_Identity=27.930174563591, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=401, Percent_Identity=27.930174563591, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571643, Length=476, Percent_Identity=26.4705882352941, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137564, Length=383, Percent_Identity=25.3263707571802, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50728; Mature: 50597

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTLYDKLWNSHVVHTEEDGTALLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV
CCHHHHHHHCCCCEEEECCCCCEEEEEEHHHHHHCCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRTHGIADPVSKLQVDTLDANCDSYGITQFKMNDLRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNLLVKVEGQLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
KDIVLAIIGRIGTAGGTGYAIEFGGSMIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTI
HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHH
DYLKGRPFSPTGAEWDQAVQYWRTFVSDPGAQFDRVVELTAADIVPQVTWGTSPEMVTAV
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DGRVPDPEREKDPVKRNAMERALAYMALEPNTPIESIQVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVAANVRLAMVVPGSGLVKAQAEREGLDKVFLEAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRRLG
CCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AQTLYDKLWNSHVVHTEEDGTALLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV
CHHHHHHHCCCCEEEECCCCCEEEEEEHHHHHHCCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRTHGIADPVSKLQVDTLDANCDSYGITQFKMNDLRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNLLVKVEGQLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
KDIVLAIIGRIGTAGGTGYAIEFGGSMIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTI
HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHH
DYLKGRPFSPTGAEWDQAVQYWRTFVSDPGAQFDRVVELTAADIVPQVTWGTSPEMVTAV
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DGRVPDPEREKDPVKRNAMERALAYMALEPNTPIESIQVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVAANVRLAMVVPGSGLVKAQAEREGLDKVFLEAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRRLG
CCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA