Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 238024250

GI number: 238024250

Start: 1010717

End: 1011346

Strand: Direct

Name: alaS [H]

Synonym: bglu_2g08320

Alternate gene names: 238024250

Gene position: 1010717-1011346 (Clockwise)

Preceding gene: 238024248

Following gene: 238024251

Centisome position: 35.75

GC content: 73.33

Gene sequence:

>630_bases
ATGACGATTCGACGCTATTACGACAGCGATGCGCTCGCGCTCGATACCGAGGTGCTGGCCTGCGAGCCCGACGGCGACGG
CCATTTTCGCGTGAGGCTGGCCGAGACGCCGTTCCACCCGCAGGGCGGCGGCCAGCCGTCCGACACCGGCCGGATCGGCG
CGGCGCGCGTGCTGCACGTGGCCGCCGAGGGCGAGGCGGTCGTGCATCGCACCGATCGCGCGGTGGCGCCCGGCGCGGCG
GCCGTGGAGGTCGACGCGGCGCCGCGCGCGCTGCATGCGCGGCTGCATTCGGCCGGCCATCTGATCGGCTATGCGGCTGC
GGCGTCGGGCTGGCGCGCGGTGAAGGGGCACCACTGGCCCGGCGAGGCGCGGGTCGTGTTCGAGCCGGGCGAGGGCGCGC
GGCCGATCGATGCCGAGACGCTCGCGCGCGAGGTGAATGCCGTGGTGGCGGCCGCGCTGCCGCGCACGACGCGCATGCGC
GACGGCATGCGGGTGATCGGGTTTGGCGACTTCGAGGCCCAGGGCTGCGGCGGCACGCACGTGGCGTCAAGCGCGCAGGT
CGGACGGATCGTGATCGTCAAGGTCAAGGAGAAGAAGGGGCAACTGACGGTGCAGTACGAGGTGGCCTGA

Upstream 100 bases:

>100_bases
TTGTAAAAAATTGAATGGCCCGCCCCCTTGAGCGGCGGCGCCGGCGCCCGCATAGTCGGGGCCTCGATGGCATCGCGATG
ACATTCGCCCAGGAGCTTTG

Downstream 100 bases:

>100_bases
GCGAGGCGGCCCGCGCGCCGTGCCCGGCTCGCGCTGCCGCCGGCCGGGGCGCGGCGTGCCCGACAGCGCTGGCCTCGCTT
TCGGCCGGGCTACCGGGTAG

Product: Threonyl/alanyl tRNA synthetase SAD

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTIRRYYDSDALALDTEVLACEPDGDGHFRVRLAETPFHPQGGGQPSDTGRIGAARVLHVAAEGEAVVHRTDRAVAPGAA
AVEVDAAPRALHARLHSAGHLIGYAAAASGWRAVKGHHWPGEARVVFEPGEGARPIDAETLAREVNAVVAAALPRTTRMR
DGMRVIGFGDFEAQGCGGTHVASSAQVGRIVIVKVKEKKGQLTVQYEVA

Sequences:

>Translated_209_residues
MTIRRYYDSDALALDTEVLACEPDGDGHFRVRLAETPFHPQGGGQPSDTGRIGAARVLHVAAEGEAVVHRTDRAVAPGAA
AVEVDAAPRALHARLHSAGHLIGYAAAASGWRAVKGHHWPGEARVVFEPGEGARPIDAETLAREVNAVVAAALPRTTRMR
DGMRVIGFGDFEAQGCGGTHVASSAQVGRIVIVKVKEKKGQLTVQYEVA
>Mature_208_residues
TIRRYYDSDALALDTEVLACEPDGDGHFRVRLAETPFHPQGGGQPSDTGRIGAARVLHVAAEGEAVVHRTDRAVAPGAAA
VEVDAAPRALHARLHSAGHLIGYAAAASGWRAVKGHHWPGEARVVFEPGEGARPIDAETLAREVNAVVAAALPRTTRMRD
GMRVIGFGDFEAQGCGGTHVASSAQVGRIVIVKVKEKKGQLTVQYEVA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR022429
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 22065; Mature: 21934

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRRYYDSDALALDTEVLACEPDGDGHFRVRLAETPFHPQGGGQPSDTGRIGAARVLHV
CCEEEEECCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
AAEGEAVVHRTDRAVAPGAAAVEVDAAPRALHARLHSAGHLIGYAAAASGWRAVKGHHWP
ECCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEECCCCCCC
GEARVVFEPGEGARPIDAETLAREVNAVVAAALPRTTRMRDGMRVIGFGDFEAQGCGGTH
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCC
VASSAQVGRIVIVKVKEKKGQLTVQYEVA
CCCCCCCCEEEEEEEECCCCCEEEEEEEC
>Mature Secondary Structure 
TIRRYYDSDALALDTEVLACEPDGDGHFRVRLAETPFHPQGGGQPSDTGRIGAARVLHV
CEEEEECCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
AAEGEAVVHRTDRAVAPGAAAVEVDAAPRALHARLHSAGHLIGYAAAASGWRAVKGHHWP
ECCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEECCCCCCC
GEARVVFEPGEGARPIDAETLAREVNAVVAAALPRTTRMRDGMRVIGFGDFEAQGCGGTH
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCC
VASSAQVGRIVIVKVKEKKGQLTVQYEVA
CCCCCCCCEEEEEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA