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The map label for this gene is iscS [H]

Identifier: 238024150

GI number: 238024150

Start: 864578

End: 866059

Strand: Reverse

Name: iscS [H]

Synonym: bglu_2g07280

Alternate gene names: 238024150

Gene position: 866059-864578 (Counterclockwise)

Preceding gene: 238024156

Following gene: 238024147

Centisome position: 30.63

GC content: 72.81

Gene sequence:

>1482_bases
ATGACCGAGAACAATACACTGCACTACCTCGATTACGCTGCCACCACGCCGGTCGACCCGCGCGTGATCGAATCGATGGC
GGCCTGCCTCGGCGCCGACGGCATCTTCGGCAACCCCGCCTCGAATTCGCATCGCGCCGGGCGGCTCGCGCGCGACAAGG
TCGAGCACGCGCGTGCCCAGGTGGCCGCGTTGATCGGCGCCGACGCGGACGAGATCGTCTGGACCTCGGGGGCCACCGAA
TCGAACAACCTCGCGCTCAAGGGCTACGCGGACCAGGCCGCCGACCGGCGCCACCTGGTGACGAGCCGCATCGAGCACAA
GGCCGTGCTCGACACGATGGAGAGCCTTGCCTCGCGCGGCATGCCGGTGGACTACGTCGCGCCGACGCGCGACGGCGAGA
TCCGCCCCGAGGACGTGGCCGCGGCGCTTCGCCCCGACACGGGGCTCGTCTCGCTGATGCTCGTGAACAACGAGATCGGC
ACGCTCACCGACGTCGGCGCGATCGCGCGCCTGGTCCACGACGCGGGCGCCCTGCTCCACGTGGACGCGGCGCAGGCGCT
CGGCAAGACGCCGATCGACGTGCATGCGCTCGGCATCGACCTGCTGTCGATGTCGGCGCACAAGGTGTACGGGCCGAAGG
GAATCGGCGCGCTGTACGTGCGGCGCGGCCTGGCCGGCCGGATCGCGCCGCAGATGCACGGCGGCGGCCATGAATCGGGG
CTGCGCTCGGGCACGCTCGCCACCCACCAGATCGTCGGCATGGGCCACGCCTGCGAGCTGGCGGCCCGCGAACTCGAGAG
CGACACGGCGCGGATCACCGCGCTCGCCACGCGCCTGCGCGACGCGGTGCTCGCGATCGGCGGCGTCGAGCAGAACGCGC
GCGCCGCGCGCCGCATTCCGCATACGCTGAGCCTGACCGTGACCGCGCCCGGCTTCCACCCCTTCATGCTCGACGAGGGG
CTCGCCGTGTCGTCGACCTCGGCCTGCAATTCGGCGGCCGGCAAGCCTTCGCACGTGCTGACCGCGCTCGGCCTCGACGC
CGCCGCCGCCGGCCGCACGGTGCGCATCAGCGTGGGCCGCTTCACGGGCGAGGACGACATCACGTTCGCCATCGACAGCT
TCCGGCGCGCCATCGAGCAATGCCGCGCCACGGCGCAAAGCGGCTTCGCCGCCTCGCACCAGATCCAGCCGGACGACCTG
AAGGCGATCCGCGGCGCGGGCTTCCGTGCCGTGATCTGCAACCGTCCCGACGGCGAAAGCGCCGACCAGCCGGCCTTCGC
GGAGATCGCTGCGGCCGCGCGCGAGCTGGGCCTCGACGCGCGCTATCTGCCGGTCGAGGCGGCCGACCGGATCGGCGAGG
CGCAGGTGGACGCGTTCGGCGCCATGCTCGACGCGCTGCCGAAGCCGGTGCTGGCCTACTGCGGCAGCGGCCGCCGCGCG
GGCCTGCTGTGGGAGCGGCTGGCGCTGCGGCGCACGGCCTGA

Upstream 100 bases:

>100_bases
TCTATGCTTTGCGTGAATTGATCGCAACTTTGGAGTTTTTTTCCCGGTCGGCGCTCGTATCATTTCGCATGCTTTAGACG
AGTAATGGCACGCCACGGAC

Downstream 100 bases:

>100_bases
GGGGCGCGCCGGACGCGCCGGATTCGCGCTGCATGCCGAGCGGCGCACGGCGGATTGTGACGGCGGGCAGCGCGGCGCCG
CTGCAGCCGCCCGACGCATC

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTENNTLHYLDYAATTPVDPRVIESMAACLGADGIFGNPASNSHRAGRLARDKVEHARAQVAALIGADADEIVWTSGATE
SNNLALKGYADQAADRRHLVTSRIEHKAVLDTMESLASRGMPVDYVAPTRDGEIRPEDVAAALRPDTGLVSLMLVNNEIG
TLTDVGAIARLVHDAGALLHVDAAQALGKTPIDVHALGIDLLSMSAHKVYGPKGIGALYVRRGLAGRIAPQMHGGGHESG
LRSGTLATHQIVGMGHACELAARELESDTARITALATRLRDAVLAIGGVEQNARAARRIPHTLSLTVTAPGFHPFMLDEG
LAVSSTSACNSAAGKPSHVLTALGLDAAAAGRTVRISVGRFTGEDDITFAIDSFRRAIEQCRATAQSGFAASHQIQPDDL
KAIRGAGFRAVICNRPDGESADQPAFAEIAAAARELGLDARYLPVEAADRIGEAQVDAFGAMLDALPKPVLAYCGSGRRA
GLLWERLALRRTA

Sequences:

>Translated_493_residues
MTENNTLHYLDYAATTPVDPRVIESMAACLGADGIFGNPASNSHRAGRLARDKVEHARAQVAALIGADADEIVWTSGATE
SNNLALKGYADQAADRRHLVTSRIEHKAVLDTMESLASRGMPVDYVAPTRDGEIRPEDVAAALRPDTGLVSLMLVNNEIG
TLTDVGAIARLVHDAGALLHVDAAQALGKTPIDVHALGIDLLSMSAHKVYGPKGIGALYVRRGLAGRIAPQMHGGGHESG
LRSGTLATHQIVGMGHACELAARELESDTARITALATRLRDAVLAIGGVEQNARAARRIPHTLSLTVTAPGFHPFMLDEG
LAVSSTSACNSAAGKPSHVLTALGLDAAAAGRTVRISVGRFTGEDDITFAIDSFRRAIEQCRATAQSGFAASHQIQPDDL
KAIRGAGFRAVICNRPDGESADQPAFAEIAAAARELGLDARYLPVEAADRIGEAQVDAFGAMLDALPKPVLAYCGSGRRA
GLLWERLALRRTA
>Mature_492_residues
TENNTLHYLDYAATTPVDPRVIESMAACLGADGIFGNPASNSHRAGRLARDKVEHARAQVAALIGADADEIVWTSGATES
NNLALKGYADQAADRRHLVTSRIEHKAVLDTMESLASRGMPVDYVAPTRDGEIRPEDVAAALRPDTGLVSLMLVNNEIGT
LTDVGAIARLVHDAGALLHVDAAQALGKTPIDVHALGIDLLSMSAHKVYGPKGIGALYVRRGLAGRIAPQMHGGGHESGL
RSGTLATHQIVGMGHACELAARELESDTARITALATRLRDAVLAIGGVEQNARAARRIPHTLSLTVTAPGFHPFMLDEGL
AVSSTSACNSAAGKPSHVLTALGLDAAAAGRTVRISVGRFTGEDDITFAIDSFRRAIEQCRATAQSGFAASHQIQPDDLK
AIRGAGFRAVICNRPDGESADQPAFAEIAAAARELGLDARYLPVEAADRIGEAQVDAFGAMLDALPKPVLAYCGSGRRAG
LLWERLALRRTA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=41.6040100250627, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=33.0097087378641, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=50.531914893617, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1787970, Length=234, Percent_Identity=31.1965811965812, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789175, Length=406, Percent_Identity=27.8325123152709, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=378, Percent_Identity=42.0634920634921, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17533177, Length=331, Percent_Identity=32.9305135951662, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=407, Percent_Identity=40.2948402948403, Blast_Score=290, Evalue=4e-79,
Organism=Drosophila melanogaster, GI20129463, Length=377, Percent_Identity=43.236074270557, Blast_Score=288, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 51799; Mature: 51668

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENNTLHYLDYAATTPVDPRVIESMAACLGADGIFGNPASNSHRAGRLARDKVEHARAQ
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCHHHHHHHHHHHHH
VAALIGADADEIVWTSGATESNNLALKGYADQAADRRHLVTSRIEHKAVLDTMESLASRG
HHHHHCCCCHHEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPVDYVAPTRDGEIRPEDVAAALRPDTGLVSLMLVNNEIGTLTDVGAIARLVHDAGALLH
CCEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
VDAAQALGKTPIDVHALGIDLLSMSAHKVYGPKGIGALYVRRGLAGRIAPQMHGGGHESG
EEHHHHHCCCCCEEEEEHHHHHHHCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
LRSGTLATHQIVGMGHACELAARELESDTARITALATRLRDAVLAIGGVEQNARAARRIP
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
HTLSLTVTAPGFHPFMLDEGLAVSSTSACNSAAGKPSHVLTALGLDAAAAGRTVRISVGR
CEEEEEEECCCCCCHHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCHHHCCCEEEEEECC
FTGEDDITFAIDSFRRAIEQCRATAQSGFAASHQIQPDDLKAIRGAGFRAVICNRPDGES
CCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
ADQPAFAEIAAAARELGLDARYLPVEAADRIGEAQVDAFGAMLDALPKPVLAYCGSGRRA
CCCCHHHHHHHHHHHHCCCCEECCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCCCCC
GLLWERLALRRTA
HHHHHHHHHHCCC
>Mature Secondary Structure 
TENNTLHYLDYAATTPVDPRVIESMAACLGADGIFGNPASNSHRAGRLARDKVEHARAQ
CCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCHHHHHHHHHHHHH
VAALIGADADEIVWTSGATESNNLALKGYADQAADRRHLVTSRIEHKAVLDTMESLASRG
HHHHHCCCCHHEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPVDYVAPTRDGEIRPEDVAAALRPDTGLVSLMLVNNEIGTLTDVGAIARLVHDAGALLH
CCEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
VDAAQALGKTPIDVHALGIDLLSMSAHKVYGPKGIGALYVRRGLAGRIAPQMHGGGHESG
EEHHHHHCCCCCEEEEEHHHHHHHCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
LRSGTLATHQIVGMGHACELAARELESDTARITALATRLRDAVLAIGGVEQNARAARRIP
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
HTLSLTVTAPGFHPFMLDEGLAVSSTSACNSAAGKPSHVLTALGLDAAAAGRTVRISVGR
CEEEEEEECCCCCCHHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCHHHCCCEEEEEECC
FTGEDDITFAIDSFRRAIEQCRATAQSGFAASHQIQPDDLKAIRGAGFRAVICNRPDGES
CCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
ADQPAFAEIAAAARELGLDARYLPVEAADRIGEAQVDAFGAMLDALPKPVLAYCGSGRRA
CCCCHHHHHHHHHHHHCCCCEECCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCCCCC
GLLWERLALRRTA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA