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The map label for this gene is rhtB [C]

Identifier: 238023874

GI number: 238023874

Start: 494671

End: 495300

Strand: Direct

Name: rhtB [C]

Synonym: bglu_2g04130

Alternate gene names: 238023874

Gene position: 494671-495300 (Clockwise)

Preceding gene: 238023872

Following gene: 238023875

Centisome position: 17.5

GC content: 72.38

Gene sequence:

>630_bases
ATGATTTCGCCTCATCTGCTCGCCCTCTACATCGCGGCGCTCGCCGCCGTCTACGCGCTTCCCGGCCCCGACATGGCGCT
GCTGCTGCAGACCAGCATCAATCGCGGCGTGCGCCCCGGCCTCGCGGCGGCCGGCGGCCTCGCGCTCGCGCGGGCCGTTC
ACGTGACGCTGTCGGCCTGCGGCGTCGCAGCGCTGCTGCGCAACGCGCCGTGGCTCTACGACGTGGTGCGCTACGGCGGC
GCGGCCTATCTCGCCTACGTGGCCTGGCAGGTGTTCCGCTCGCCGCCGTTCGCGCTCGCGGGCGAGGCGCTGGCCACGCG
CGCCGGCCTGCCGCTGCGCCGGCATTTCACCAAGGGCCTGCTCACCAACCTGCTGAACCCGAAGGCGCTGCTGTTCTGCT
CGGTGCTGCTGCCGCAGTTCGTGCGGCCGGCCGCGGGCGCGGTGGGCTGGCAGATGACCGAACTCGGCGTGCTGCTGATC
GTGACCGGCGCACTGTTCGATGCCGCCTACGTGATCGGCGCCTCGCGGATCTCGGGCTGGATGCGCGCGCACCCGCTCGC
GCAGACGCTGCAACGCTGGACTTTCGCCACCGCGCTGTTCGCGTTCGCCTGCCGGCTGTCGGTCGACTGA

Upstream 100 bases:

>100_bases
CCATCCCGCGCGCCGGCCTTGCTGCGGCGGCCGCGCGCGTTTTCAGGCCGCTTCCGAGCCGGCTTTCCCGTCCTTGTGCT
TCTTTCGCCCGATTCACGCC

Downstream 100 bases:

>100_bases
CGGCGCACCCGGCGGCGGCTTGCGCGGGCGGGCCGCCGCGCGCGTGCTCGAGCCGTTCACGATGCGGCCGGCCGCTCGGC
CGACCTGGACGGCGAGCCGC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MISPHLLALYIAALAAVYALPGPDMALLLQTSINRGVRPGLAAAGGLALARAVHVTLSACGVAALLRNAPWLYDVVRYGG
AAYLAYVAWQVFRSPPFALAGEALATRAGLPLRRHFTKGLLTNLLNPKALLFCSVLLPQFVRPAAGAVGWQMTELGVLLI
VTGALFDAAYVIGASRISGWMRAHPLAQTLQRWTFATALFAFACRLSVD

Sequences:

>Translated_209_residues
MISPHLLALYIAALAAVYALPGPDMALLLQTSINRGVRPGLAAAGGLALARAVHVTLSACGVAALLRNAPWLYDVVRYGG
AAYLAYVAWQVFRSPPFALAGEALATRAGLPLRRHFTKGLLTNLLNPKALLFCSVLLPQFVRPAAGAVGWQMTELGVLLI
VTGALFDAAYVIGASRISGWMRAHPLAQTLQRWTFATALFAFACRLSVD
>Mature_209_residues
MISPHLLALYIAALAAVYALPGPDMALLLQTSINRGVRPGLAAAGGLALARAVHVTLSACGVAALLRNAPWLYDVVRYGG
AAYLAYVAWQVFRSPPFALAGEALATRAGLPLRRHFTKGLLTNLLNPKALLFCSVLLPQFVRPAAGAVGWQMTELGVLLI
VTGALFDAAYVIGASRISGWMRAHPLAQTLQRWTFATALFAFACRLSVD

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=173, Percent_Identity=30.635838150289, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788099, Length=189, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI48994978, Length=134, Percent_Identity=29.1044776119403, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22211; Mature: 22211

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPHLLALYIAALAAVYALPGPDMALLLQTSINRGVRPGLAAAGGLALARAVHVTLSAC
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVAALLRNAPWLYDVVRYGGAAYLAYVAWQVFRSPPFALAGEALATRAGLPLRRHFTKGL
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
LTNLLNPKALLFCSVLLPQFVRPAAGAVGWQMTELGVLLIVTGALFDAAYVIGASRISGW
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRAHPLAQTLQRWTFATALFAFACRLSVD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISPHLLALYIAALAAVYALPGPDMALLLQTSINRGVRPGLAAAGGLALARAVHVTLSAC
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVAALLRNAPWLYDVVRYGGAAYLAYVAWQVFRSPPFALAGEALATRAGLPLRRHFTKGL
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
LTNLLNPKALLFCSVLLPQFVRPAAGAVGWQMTELGVLLIVTGALFDAAYVIGASRISGW
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRAHPLAQTLQRWTFATALFAFACRLSVD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]