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The map label for this gene is bkdA1 [H]

Identifier: 238023732

GI number: 238023732

Start: 309866

End: 311098

Strand: Direct

Name: bkdA1 [H]

Synonym: bglu_2g02640

Alternate gene names: 238023732

Gene position: 309866-311098 (Clockwise)

Preceding gene: 238023731

Following gene: 238023733

Centisome position: 10.96

GC content: 68.29

Gene sequence:

>1233_bases
ATGAGCCAATACGGGCCATTGAAATTGCATGTGCCGGAGCCGACCGGGCGGCCGGGCTGCAAAACCGACTTTTCCTATCT
GCAACTTTCTCCGGCCGGCCAGGTGCGCAGGCCGCCGATCGACGTCACGCCGGTGGATACCAGCGACCTGGCCTACGGCC
TGGTGCGGGTGCTCGACGACGATGGACGCGCGGTCGGCCCCTGGGCTCCCGAACTCGGCCGCGAGCGGCTGCGCGCCGGC
ATGCACGCGATGCTCAAGACGCGCGTGTTCGATTCGCGAATGCTGATCGCGCAGCGCCAGCGCAAGATCTCGTTCTACAT
GCAAAGCCTCGGCGAGGAGGCGATCGGCACCGCCCATAGCTTCGCGCTCGACAACGGCGACATGTGCTTTCCGAGCTACC
GCCAGCAAAGCATCCTGATCACGCGCGAGGTGCCGCTCGTCGATCTGATGTGCCAACTGATGTCGAACGACCGCGATCCG
CTCAAGGGCCGCCAGCTGCCCGTGATGTACTCGAACCGCGCGGCCGGCTTCTTCACGATCTCGGGCAACCTCGCCACCCA
GTTCATCCAGGCGGTGGGCTGGGCGATGGCCTCGGCGATCAAGGGCGACACGCGCATCGCGTCGGCCTGGATCGGCGACG
GCGCCACCGCCGAGGCCGACTTCCACACCGCGCTGACCTTCGCCCACGTCTACCGCGCGCCGGTGATCCTCAACGTGGTC
AACAACCAGTGGGCGATCTCCACGTTCCAGGCGATCGCGGGCGGCGAGGGCGCGACCTTCGCCGGGCGCGGCGTGGGCTG
CGGGATCGCCTCGCTGCGGGTGGACGGCAACGACTTCCTGGCGGTCTACGCGGCCTCGCGCTGGGCCGCCGAACGCGCGC
GCCGCAACCTCGGCCCGACGCTGATCGAATGGGTCACCTATCGCGCCGGCCCGCACTCGACCTCGGACGATCCGTCGAAG
TACCGCCCCGGCGACGACTGGACCCATTTCCCGCTCGGCGATCCGATCGAACGCTTCAAGCGCCACCTGATCGGCGAGGG
CATCTGGTCCGAGCAGGAGCACGCCGACCTGAAGGGCGCGCTGGAGGCCGAGGTGATCGCCGCGCAGAAGGAGGCGGAGC
GCTTCGGCACGCTCGGCGACGACCGCGTCGCCGACGTGGCCAGCTTCTTCGAGGATGTCTACAAGGACATGCCGGAACAC
CTGCGGCGCCAGCGCCAGCAACTCGGAGTCTGA

Upstream 100 bases:

>100_bases
ATCGGTGCGACTCCGGGACGAAAGGCTCTATCACTCGGTGCGGCGAATTGTTACGATCACCGCCGAAGCGGGCGACAAAA
TCAAACGTGGAGATGATTAA

Downstream 100 bases:

>100_bases
CCGATGACCACAACACAACCGAAGGGTCAGTCGGCCACGACCATGACCATGATCCAGGCATTGCGCTCCGCGATGGACGT
GATGCTCGAGCGCAGCAGCG

Product: 2-oxoisovalerate dehydrogenase, E1 component subunit alpha

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSQYGPLKLHVPEPTGRPGCKTDFSYLQLSPAGQVRRPPIDVTPVDTSDLAYGLVRVLDDDGRAVGPWAPELGRERLRAG
MHAMLKTRVFDSRMLIAQRQRKISFYMQSLGEEAIGTAHSFALDNGDMCFPSYRQQSILITREVPLVDLMCQLMSNDRDP
LKGRQLPVMYSNRAAGFFTISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPSK
YRPGDDWTHFPLGDPIERFKRHLIGEGIWSEQEHADLKGALEAEVIAAQKEAERFGTLGDDRVADVASFFEDVYKDMPEH
LRRQRQQLGV

Sequences:

>Translated_410_residues
MSQYGPLKLHVPEPTGRPGCKTDFSYLQLSPAGQVRRPPIDVTPVDTSDLAYGLVRVLDDDGRAVGPWAPELGRERLRAG
MHAMLKTRVFDSRMLIAQRQRKISFYMQSLGEEAIGTAHSFALDNGDMCFPSYRQQSILITREVPLVDLMCQLMSNDRDP
LKGRQLPVMYSNRAAGFFTISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPSK
YRPGDDWTHFPLGDPIERFKRHLIGEGIWSEQEHADLKGALEAEVIAAQKEAERFGTLGDDRVADVASFFEDVYKDMPEH
LRRQRQQLGV
>Mature_409_residues
SQYGPLKLHVPEPTGRPGCKTDFSYLQLSPAGQVRRPPIDVTPVDTSDLAYGLVRVLDDDGRAVGPWAPELGRERLRAGM
HAMLKTRVFDSRMLIAQRQRKISFYMQSLGEEAIGTAHSFALDNGDMCFPSYRQQSILITREVPLVDLMCQLMSNDRDPL
KGRQLPVMYSNRAAGFFTISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVVN
NQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPSKY
RPGDDWTHFPLGDPIERFKRHLIGEGIWSEQEHADLKGALEAEVIAAQKEAERFGTLGDDRVADVASFFEDVYKDMPEHL
RRQRQQLGV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=358, Percent_Identity=38.268156424581, Blast_Score=225, Evalue=7e-59,
Organism=Homo sapiens, GI258645172, Length=359, Percent_Identity=38.1615598885794, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI291084742, Length=269, Percent_Identity=26.3940520446097, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4505685, Length=269, Percent_Identity=26.3940520446097, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4885543, Length=257, Percent_Identity=25.2918287937743, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI291084744, Length=274, Percent_Identity=25.9124087591241, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI86563357, Length=328, Percent_Identity=39.0243902439024, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI86563355, Length=328, Percent_Identity=39.0243902439024, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17536047, Length=303, Percent_Identity=26.0726072607261, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32564172, Length=303, Percent_Identity=26.0726072607261, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321026, Length=347, Percent_Identity=29.6829971181556, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355903, Length=365, Percent_Identity=34.5205479452055, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24639748, Length=327, Percent_Identity=26.2996941896024, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639740, Length=291, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24639746, Length=291, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24639744, Length=291, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571106, Length=291, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45299; Mature: 45168

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYGPLKLHVPEPTGRPGCKTDFSYLQLSPAGQVRRPPIDVTPVDTSDLAYGLVRVLDD
CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DGRAVGPWAPELGRERLRAGMHAMLKTRVFDSRMLIAQRQRKISFYMQSLGEEAIGTAHS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHH
FALDNGDMCFPSYRQQSILITREVPLVDLMCQLMSNDRDPLKGRQLPVMYSNRAAGFFTI
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEE
SGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
ECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPT
CCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHH
LIEWVTYRAGPHSTSDDPSKYRPGDDWTHFPLGDPIERFKRHLIGEGIWSEQEHADLKGA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LEAEVIAAQKEAERFGTLGDDRVADVASFFEDVYKDMPEHLRRQRQQLGV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQYGPLKLHVPEPTGRPGCKTDFSYLQLSPAGQVRRPPIDVTPVDTSDLAYGLVRVLDD
CCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DGRAVGPWAPELGRERLRAGMHAMLKTRVFDSRMLIAQRQRKISFYMQSLGEEAIGTAHS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHH
FALDNGDMCFPSYRQQSILITREVPLVDLMCQLMSNDRDPLKGRQLPVMYSNRAAGFFTI
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEE
SGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
ECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPT
CCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHH
LIEWVTYRAGPHSTSDDPSKYRPGDDWTHFPLGDPIERFKRHLIGEGIWSEQEHADLKGA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LEAEVIAAQKEAERFGTLGDDRVADVASFFEDVYKDMPEHLRRQRQQLGV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]