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The map label for this gene is bkdA2 [H]

Identifier: 238023733

GI number: 238023733

Start: 311141

End: 312145

Strand: Direct

Name: bkdA2 [H]

Synonym: bglu_2g02650

Alternate gene names: 238023733

Gene position: 311141-312145 (Clockwise)

Preceding gene: 238023732

Following gene: 238023734

Centisome position: 11.0

GC content: 68.96

Gene sequence:

>1005_bases
ATGACCATGATCCAGGCATTGCGCTCCGCGATGGACGTGATGCTCGAGCGCAGCAGCGACGTGGTGGTGTTCGGCCAGGA
CGTCGGCTATTTCGGCGGCGTGTTCCGCTGCACCGAGGGCCTGCAGGCGAAGTTCGGCAACTCGCGCGTGTTCGACACGC
CGATCTCCGAGGGCGGCATCGTCGGCGTGGCGGTGGGGATGGGCGCCTACGGGCTGCGGCCCGTCTGCGAGATCCAGTTC
GCCGACTACTTCTATCCGGCCTCCGACCAGATCGTCTCCGAGGCCGCGCGCCTGCGCTACCGCTCGGCGGCCGAGTTCAC
CGCGCCGCTCACCATCCGCATGCCGTGCGGCGGCGGCATCTACGGCGGCCAGACGCACAGCCAGAGCCCCGAGGCCATGT
TTACGCAGGTCTGCGGGCTGCGCACGGTGATGCCGTCGAACCCTTACGACGCCAAGGGCCTCTTGATCGCGGCGATCGAG
AACGACGATCCGGTGATCTTCCTCGAACCGAAGCGTCTCTACAACGGCCCGTTCGACGGCCACCACGAGCGCCCCGTCAC
GCCGTGGAACCAGCATCCGGCGAGTCTCGTGCCGGAGGGCTACTACACCGTGCCGCTCGAAACCGCCGCCGTGGTGCGGC
CCGGCGAGGCGCTGACGGTGCTGACCTACGGCACCACCGTGCACGTCTCGCTCGCGGCCGCGCAGGAAACCGGCATCGAC
GCCGAGGTGATCGATCTGCGCAGCCTCTGGCCGCTCGATCTCGACGCGATCGTCGAGTCGGTGCGCAAGACCGGGCGCTG
CGTGGTGGTGCACGAGGCCACTCGCACCTGCGGGTTCGGCGCCGAGTTGATCGCGCTCGTCCAGGAGCACTGCTTCCACT
GGCTCGAGGCGCCGGTGGAGCGCGTCACCGGCTGGGACACGCCTTATCCGCATGCGCAGGAGTGGGCGTACTTTCCGGGG
CCGAGCCGCGTGGGCGAGGCGATGCGACGCGTGATGGAGAGCTGA

Upstream 100 bases:

>100_bases
TGTCTACAAGGACATGCCGGAACACCTGCGGCGCCAGCGCCAGCAACTCGGAGTCTGACCGATGACCACAACACAACCGA
AGGGTCAGTCGGCCACGACC

Downstream 100 bases:

>100_bases
GATGGGTATCCATGTGATCAAGATGCCGGACATCGGCGAAGGCATCGCGGAAGTCGAGCTGGGGCTGTGGCACGTGAAGG
TGGGCGACCGCGTCGTGGAG

Product: 2-oxoisovalerate dehydrogenase, E1 component subunit beta

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTMIQALRSAMDVMLERSSDVVVFGQDVGYFGGVFRCTEGLQAKFGNSRVFDTPISEGGIVGVAVGMGAYGLRPVCEIQF
ADYFYPASDQIVSEAARLRYRSAAEFTAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIAAIE
NDDPVIFLEPKRLYNGPFDGHHERPVTPWNQHPASLVPEGYYTVPLETAAVVRPGEALTVLTYGTTVHVSLAAAQETGID
AEVIDLRSLWPLDLDAIVESVRKTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDTPYPHAQEWAYFPG
PSRVGEAMRRVMES

Sequences:

>Translated_334_residues
MTMIQALRSAMDVMLERSSDVVVFGQDVGYFGGVFRCTEGLQAKFGNSRVFDTPISEGGIVGVAVGMGAYGLRPVCEIQF
ADYFYPASDQIVSEAARLRYRSAAEFTAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIAAIE
NDDPVIFLEPKRLYNGPFDGHHERPVTPWNQHPASLVPEGYYTVPLETAAVVRPGEALTVLTYGTTVHVSLAAAQETGID
AEVIDLRSLWPLDLDAIVESVRKTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDTPYPHAQEWAYFPG
PSRVGEAMRRVMES
>Mature_333_residues
TMIQALRSAMDVMLERSSDVVVFGQDVGYFGGVFRCTEGLQAKFGNSRVFDTPISEGGIVGVAVGMGAYGLRPVCEIQFA
DYFYPASDQIVSEAARLRYRSAAEFTAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIAAIEN
DDPVIFLEPKRLYNGPFDGHHERPVTPWNQHPASLVPEGYYTVPLETAAVVRPGEALTVLTYGTTVHVSLAAAQETGIDA
EVIDLRSLWPLDLDAIVESVRKTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDTPYPHAQEWAYFPGP
SRVGEAMRRVMES

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=338, Percent_Identity=44.9704142011834, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI34101272, Length=338, Percent_Identity=44.9704142011834, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI156564403, Length=317, Percent_Identity=35.6466876971609, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI291084858, Length=317, Percent_Identity=34.384858044164, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=46.2686567164179, Blast_Score=277, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17538422, Length=339, Percent_Identity=38.0530973451327, Blast_Score=210, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=339, Percent_Identity=36.5781710914454, Blast_Score=215, Evalue=9e-57,
Organism=Drosophila melanogaster, GI160714832, Length=338, Percent_Identity=45.2662721893491, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI160714828, Length=338, Percent_Identity=45.2662721893491, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI21358145, Length=317, Percent_Identity=39.1167192429022, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24650940, Length=317, Percent_Identity=39.1167192429022, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=42.6966292134831, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=42.6966292134831, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI160714826, Length=65, Percent_Identity=47.6923076923077, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36563; Mature: 36432

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIQALRSAMDVMLERSSDVVVFGQDVGYFGGVFRCTEGLQAKFGNSRVFDTPISEGGI
CHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCHHHHHHHHHHHHCCCCEEEECCCCCCCE
VGVAVGMGAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFTAPLTIRMPCGGGI
EEEEECCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
YGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIAAIENDDPVIFLEPKRLYNGPFDG
CCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCEEEECCHHHHCCCCCC
HHERPVTPWNQHPASLVPEGYYTVPLETAAVVRPGEALTVLTYGTTVHVSLAAAQETGID
CCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEEEEEEECCEEEEEEEEHHHCCCC
AEVIDLRSLWPLDLDAIVESVRKTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVE
CEEEEHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVTGWDTPYPHAQEWAYFPGPSRVGEAMRRVMES
HHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMIQALRSAMDVMLERSSDVVVFGQDVGYFGGVFRCTEGLQAKFGNSRVFDTPISEGGI
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHCHHHHHHHHHHHHCCCCEEEECCCCCCCE
VGVAVGMGAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFTAPLTIRMPCGGGI
EEEEECCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
YGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIAAIENDDPVIFLEPKRLYNGPFDG
CCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCEEEECCHHHHCCCCCC
HHERPVTPWNQHPASLVPEGYYTVPLETAAVVRPGEALTVLTYGTTVHVSLAAAQETGID
CCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEEEEEEECCEEEEEEEEHHHCCCC
AEVIDLRSLWPLDLDAIVESVRKTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVE
CEEEEHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVTGWDTPYPHAQEWAYFPGPSRVGEAMRRVMES
HHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]