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The map label for this gene is fixL [H]

Identifier: 238023589

GI number: 238023589

Start: 95449

End: 96927

Strand: Direct

Name: fixL [H]

Synonym: bglu_2g00920

Alternate gene names: 238023589

Gene position: 95449-96927 (Clockwise)

Preceding gene: 238023585

Following gene: 238023597

Centisome position: 3.38

GC content: 72.08

Gene sequence:

>1479_bases
ATGACGTTCCCCGCCGAAGACGATTTCCGCCGCCTGCTCGATTCGCTGACGCTGTGCGTGCTGCTGCACGACGCGCACAC
CAAGGCGATCGTCTGGGCCAACCGCGCCGCCTGCGACGCGCTCGGCTTCACGGTCGAGGAGCTGCTGCCGCTGAAGGCGC
ACGACATGACGCGGCCCGAGCCCAGATACGCACGCGAGAACGCGATAGCCGCGATGGATCGCGCCGAGACGCAAGGGCCG
CAGGTCTATGAGTGGTGCTACCGGTCGCGGCGCGGCGAGGACATGCTGTCCGAGGCGATCGTCACCCACGTGAAGCTGCG
CGAGCGGGCGGTGGTGATGGTGCAGTTCCGCGACATCAGCGCCGAGGAGGCGATCCGGCAGAAGCTCAGGCACTACGAAA
CGCGGCTGCGCGAATTCATGCAGGACCTCGGCGAAGGCGTCGCGGTGCTCGACGAGGCGGGCGCGATTCAGTACCTCAGC
GCGTCGGGGCGGCGCCTGCTCGCGCTCGATGCGCGAACGCCGCCGGGCGTGGTGACCGACCACCTCGATCCGCTCGACCA
CGCGCGCCTGCAGGCGCAGCTGCGCGAGGCGCCCACCGGCGGGCCGTCGGCACCGGCGCGCTACCGGATGCGCGACGGCG
CGCGCTGGCTGCGCATCACGTGCCGCCAGCTGCACGGCACCGACGATCTGGCCGGCGTGCTGATCCACTTTCGCGACGTC
ACCGACGTGGTGCGCATCGAGGACGCGCGCCGCGCCGAGGCGCGCCAGCTCGAATACGCCGGGCGCTACAACGCGATGGG
CGAGATGGCGATCGCGATCGCGCACGAACTGAGCCAGCCGCTCGCGGCGATCCGCAATTTCATCGAGGGCACGATTCAGC
GGCTCGCCGCGCCGGGCCAGCTGGACGGTGCGATCTGGGGCCTGCGCGCCGCCGACCGGCAGGCCGAGCACGCCGCCGCG
ATCATCGGCAGCGTGCGCGAGTACGTGGCGCGCCGGGCGCCGAACGTCGGGGTCGTGGACCTCGCCGCGATCCTCGCCGA
GGTGGCGTACTTCGTCGAGCTGCGCGCGCGCGATACGGGCGTGCAGGTCGCCGTCGAGCCGCCGGCGGCGGCGCTGCCGG
TCGAGTGCGAGCGCGTGCTGATCGGGCAGGTGATCCTCAATCTCGCCTTCAACGCGATCGATGCGCTCTTGGAAATGCCG
CCCGGCGCGCGGCGCCTGACGATCGCCGCCGCGCGCGACGGCGACGCCGCCATGGTGCAGGTGCGCGACAACGGGCCGGG
CGTGCCGGAGGGCGTCCATGCCGGCCTGTTCGACGGATTCTCCTCGTCCAAGCCGGCCGGCAACGGCATCGGCCTGTCGC
TCTGCAATAACATCGTGACGCGCCACGGCGGCCGGATCCGCGCGTTTGCCGCCGAGGGCGGCGGCCTCGACTGTCGCTTC
TGGCTGCCGCTGCATCGCGGCGGCGACGCGGCCCGCTAG

Upstream 100 bases:

>100_bases
CGCAGCGGACTAAAGAAATCTTCAGATGCCGCCGCGCGCGTCGCGGTGTATCGTGGCAGGCAGCAGCGCCGCCCCGAAGG
CCGCCGATTCCATCCGCCCT

Downstream 100 bases:

>100_bases
CCGCGCCGGCCGGCAACGCGGCGGCGCCGCGCGGGCGCCGCGGATGCGGTCAGAAGTTGTGGCGGATGCCGGCGATCACG
GCCATTTGGCGGTTCGTGTC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTFPAEDDFRRLLDSLTLCVLLHDAHTKAIVWANRAACDALGFTVEELLPLKAHDMTRPEPRYARENAIAAMDRAETQGP
QVYEWCYRSRRGEDMLSEAIVTHVKLRERAVVMVQFRDISAEEAIRQKLRHYETRLREFMQDLGEGVAVLDEAGAIQYLS
ASGRRLLALDARTPPGVVTDHLDPLDHARLQAQLREAPTGGPSAPARYRMRDGARWLRITCRQLHGTDDLAGVLIHFRDV
TDVVRIEDARRAEARQLEYAGRYNAMGEMAIAIAHELSQPLAAIRNFIEGTIQRLAAPGQLDGAIWGLRAADRQAEHAAA
IIGSVREYVARRAPNVGVVDLAAILAEVAYFVELRARDTGVQVAVEPPAAALPVECERVLIGQVILNLAFNAIDALLEMP
PGARRLTIAAARDGDAAMVQVRDNGPGVPEGVHAGLFDGFSSSKPAGNGIGLSLCNNIVTRHGGRIRAFAAEGGGLDCRF
WLPLHRGGDAAR

Sequences:

>Translated_492_residues
MTFPAEDDFRRLLDSLTLCVLLHDAHTKAIVWANRAACDALGFTVEELLPLKAHDMTRPEPRYARENAIAAMDRAETQGP
QVYEWCYRSRRGEDMLSEAIVTHVKLRERAVVMVQFRDISAEEAIRQKLRHYETRLREFMQDLGEGVAVLDEAGAIQYLS
ASGRRLLALDARTPPGVVTDHLDPLDHARLQAQLREAPTGGPSAPARYRMRDGARWLRITCRQLHGTDDLAGVLIHFRDV
TDVVRIEDARRAEARQLEYAGRYNAMGEMAIAIAHELSQPLAAIRNFIEGTIQRLAAPGQLDGAIWGLRAADRQAEHAAA
IIGSVREYVARRAPNVGVVDLAAILAEVAYFVELRARDTGVQVAVEPPAAALPVECERVLIGQVILNLAFNAIDALLEMP
PGARRLTIAAARDGDAAMVQVRDNGPGVPEGVHAGLFDGFSSSKPAGNGIGLSLCNNIVTRHGGRIRAFAAEGGGLDCRF
WLPLHRGGDAAR
>Mature_491_residues
TFPAEDDFRRLLDSLTLCVLLHDAHTKAIVWANRAACDALGFTVEELLPLKAHDMTRPEPRYARENAIAAMDRAETQGPQ
VYEWCYRSRRGEDMLSEAIVTHVKLRERAVVMVQFRDISAEEAIRQKLRHYETRLREFMQDLGEGVAVLDEAGAIQYLSA
SGRRLLALDARTPPGVVTDHLDPLDHARLQAQLREAPTGGPSAPARYRMRDGARWLRITCRQLHGTDDLAGVLIHFRDVT
DVVRIEDARRAEARQLEYAGRYNAMGEMAIAIAHELSQPLAAIRNFIEGTIQRLAAPGQLDGAIWGLRAADRQAEHAAAI
IGSVREYVARRAPNVGVVDLAAILAEVAYFVELRARDTGVQVAVEPPAAALPVECERVLIGQVILNLAFNAIDALLEMPP
GARRLTIAAARDGDAAMVQVRDNGPGVPEGVHAGLFDGFSSSKPAGNGIGLSLCNNIVTRHGGRIRAFAAEGGGLDCRFW
LPLHRGGDAAR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=375, Percent_Identity=24.5333333333333, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=25.3393665158371, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790300, Length=360, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53858; Mature: 53727

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPAEDDFRRLLDSLTLCVLLHDAHTKAIVWANRAACDALGFTVEELLPLKAHDMTRPE
CCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCC
PRYARENAIAAMDRAETQGPQVYEWCYRSRRGEDMLSEAIVTHVKLRERAVVMVQFRDIS
CHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCC
AEEAIRQKLRHYETRLREFMQDLGEGVAVLDEAGAIQYLSASGRRLLALDARTPPGVVTD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCCCEEEECCCCCEEEEEECCCCCCCCCC
HLDPLDHARLQAQLREAPTGGPSAPARYRMRDGARWLRITCRQLHGTDDLAGVLIHFRDV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
TDVVRIEDARRAEARQLEYAGRYNAMGEMAIAIAHELSQPLAAIRNFIEGTIQRLAAPGQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDGAIWGLRAADRQAEHAAAIIGSVREYVARRAPNVGVVDLAAILAEVAYFVELRARDTG
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VQVAVEPPAAALPVECERVLIGQVILNLAFNAIDALLEMPPGARRLTIAAARDGDAAMVQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEE
VRDNGPGVPEGVHAGLFDGFSSSKPAGNGIGLSLCNNIVTRHGGRIRAFAAEGGGLDCRF
EECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
WLPLHRGGDAAR
EEEEECCCCCCC
>Mature Secondary Structure 
TFPAEDDFRRLLDSLTLCVLLHDAHTKAIVWANRAACDALGFTVEELLPLKAHDMTRPE
CCCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCC
PRYARENAIAAMDRAETQGPQVYEWCYRSRRGEDMLSEAIVTHVKLRERAVVMVQFRDIS
CHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCC
AEEAIRQKLRHYETRLREFMQDLGEGVAVLDEAGAIQYLSASGRRLLALDARTPPGVVTD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCCCEEEECCCCCEEEEEECCCCCCCCCC
HLDPLDHARLQAQLREAPTGGPSAPARYRMRDGARWLRITCRQLHGTDDLAGVLIHFRDV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
TDVVRIEDARRAEARQLEYAGRYNAMGEMAIAIAHELSQPLAAIRNFIEGTIQRLAAPGQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDGAIWGLRAADRQAEHAAAIIGSVREYVARRAPNVGVVDLAAILAEVAYFVELRARDTG
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VQVAVEPPAAALPVECERVLIGQVILNLAFNAIDALLEMPPGARRLTIAAARDGDAAMVQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEE
VRDNGPGVPEGVHAGLFDGFSSSKPAGNGIGLSLCNNIVTRHGGRIRAFAAEGGGLDCRF
EECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
WLPLHRGGDAAR
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]