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The map label for this gene is nodW [H]

Identifier: 238023585

GI number: 238023585

Start: 91979

End: 92626

Strand: Direct

Name: nodW [H]

Synonym: bglu_2g00880

Alternate gene names: 238023585

Gene position: 91979-92626 (Clockwise)

Preceding gene: 238023583

Following gene: 238023589

Centisome position: 3.25

GC content: 64.97

Gene sequence:

>648_bases
ATGATGTCTCCCACCCCGATGATCTACATCGTCGATGATGACAGCGGCATGCGGACCTCGCTCGCCTGGCTGCTCGAGTC
GGTCGGCGTGAAGTCGGAAGGATTCGCGAGCGCGGCCGAATTTCTCGAGGCATTCGATCAGAGCGTTCCGTCGTGCCTGA
TCCTCGACGTGCGCATGCCGGAGCGCAGCGGCTTCGACGTGCAGACCGAATTGAACCGGCGCGGCGCGACCTTGCCGATC
ATCTTCGTCAGCGGACATGGCGACATACCGATGTCGGTACGGGCGATGCAGAATGGCGCGATCGATTTCGTCGAGAAGCC
CTACAACTCGCAGCAGATGCTCGACCGCGTGCAGAACGCGATGAAGCTCGCGATGCAGCGCCATGCCGGCGATCGCCGCC
GGCTGGAGCTGCGTCAGCGCATCGAATCGCTCACGGCGCGCGAGCGGGAGGTGCTGCGCGGCGTGATCGACGGCAAGGGC
AGCAAGAAGATCGCCTCCGAGCTGTCGATCAGCGTGAAGACCGTGGACGTGCACCGGGCCAGCATCAAGGAAAAGCTCGG
GGTAGCGTCGATCGCGAGCCTGGTGCGCGACGTGACCTTCGTATGGGGCGCGCCCGGGGACGGGATGAGCGGGTCGGCCG
GGCGTTGA

Upstream 100 bases:

>100_bases
CTGGTTACCATGCGAGGTCGAACGCGGCTGCGCGCGGCTGCGTGCGATACGCAAATCAGTCGCGCCGCGCGAGCCCGTTC
TGAAACATGGAGCGAGAAAC

Downstream 100 bases:

>100_bases
GCCCGGCGCGCGTGTATCGCGCCCGGTGCGCGCCGCCCGCCGGGCGCAGAACCCGCGCGCGTTGCGGCCCGGCGGCCGCA
AGCGATGCCGAGCGGTGGGC

Product: LuxR family two component transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MMSPTPMIYIVDDDSGMRTSLAWLLESVGVKSEGFASAAEFLEAFDQSVPSCLILDVRMPERSGFDVQTELNRRGATLPI
IFVSGHGDIPMSVRAMQNGAIDFVEKPYNSQQMLDRVQNAMKLAMQRHAGDRRRLELRQRIESLTAREREVLRGVIDGKG
SKKIASELSISVKTVDVHRASIKEKLGVASIASLVRDVTFVWGAPGDGMSGSAGR

Sequences:

>Translated_215_residues
MMSPTPMIYIVDDDSGMRTSLAWLLESVGVKSEGFASAAEFLEAFDQSVPSCLILDVRMPERSGFDVQTELNRRGATLPI
IFVSGHGDIPMSVRAMQNGAIDFVEKPYNSQQMLDRVQNAMKLAMQRHAGDRRRLELRQRIESLTAREREVLRGVIDGKG
SKKIASELSISVKTVDVHRASIKEKLGVASIASLVRDVTFVWGAPGDGMSGSAGR
>Mature_215_residues
MMSPTPMIYIVDDDSGMRTSLAWLLESVGVKSEGFASAAEFLEAFDQSVPSCLILDVRMPERSGFDVQTELNRRGATLPI
IFVSGHGDIPMSVRAMQNGAIDFVEKPYNSQQMLDRVQNAMKLAMQRHAGDRRRLELRQRIESLTAREREVLRGVIDGKG
SKKIASELSISVKTVDVHRASIKEKLGVASIASLVRDVTFVWGAPGDGMSGSAGR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=33.0434782608696, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790102, Length=210, Percent_Identity=30, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=26.9565217391304, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=26.4423076923077, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=122, Percent_Identity=27.0491803278689, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23582; Mature: 23582

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSPTPMIYIVDDDSGMRTSLAWLLESVGVKSEGFASAAEFLEAFDQSVPSCLILDVRMP
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ERSGFDVQTELNRRGATLPIIFVSGHGDIPMSVRAMQNGAIDFVEKPYNSQQMLDRVQNA
CCCCCCCHHHHCCCCCEEEEEEEECCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHH
MKLAMQRHAGDRRRLELRQRIESLTAREREVLRGVIDGKGSKKIASELSISVKTVDVHRA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEHHHHH
SIKEKLGVASIASLVRDVTFVWGAPGDGMSGSAGR
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure
MMSPTPMIYIVDDDSGMRTSLAWLLESVGVKSEGFASAAEFLEAFDQSVPSCLILDVRMP
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ERSGFDVQTELNRRGATLPIIFVSGHGDIPMSVRAMQNGAIDFVEKPYNSQQMLDRVQNA
CCCCCCCHHHHCCCCCEEEEEEEECCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHH
MKLAMQRHAGDRRRLELRQRIESLTAREREVLRGVIDGKGSKKIASELSISVKTVDVHRA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEHHHHH
SIKEKLGVASIASLVRDVTFVWGAPGDGMSGSAGR
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]