Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 238023557

GI number: 238023557

Start: 61642

End: 62301

Strand: Reverse

Name: folE [H]

Synonym: bglu_2g00590

Alternate gene names: 238023557

Gene position: 62301-61642 (Counterclockwise)

Preceding gene: 238023558

Following gene: 238023556

Centisome position: 2.2

GC content: 66.36

Gene sequence:

>660_bases
ATGTCGAAACGCAAGACCGCCAACAAGACGGCGGAACCCGTCGCTGCCCCGACCACCAGCGACATCGCGCGCCCGAGCCG
CGCCGAAGCCGAGGATGCCGTGCGCGTACTGCTGCGCTGGGCCGGCGACGATCCCGCCCGCGAGGGCCTGCTCGATACGC
CGGCGCGCGTGGTGCGCGCCTACGAAGAATTCTTTCGGGGCTACCAGCTCGAACCGCGCGAGATCCTCGCGCGCACCTTC
AGCGAGGTGGACGGCTACGACGAAATGATCGTGCTGAAGGACATCCGCTTCGAGAGCTATTGCGAGCACCACATGGTGCC
GATCATCGGCCGCGCGCATGTCGCGTATCTGCCGAACCACCGCGTGGTGGGCATCTCGAAGCTGGCGCGGCTCGTCGATG
CGTTCGCCAAGCGCCTGCAGATCCAGGAAAAGATGACGGTGCAGATCGCCGATACCCTGTTCGAGGTGCTGCAGCCCAAG
GGCGTCGGCGTGATCCTCGAGGCCGCGCACCAATGCATCTCGACGCGCGGCGTCCACAAGCCCGGCGTCGAGATGGTCAC
CTCGCGCATGCTCGGCACGTTCCGCAGCGATCCGGCCACGCGCCGCGAATTCCTGTCGATCGTGGCGAATCCGTCGTCGG
TCAACCTGACCAACACGTGA

Upstream 100 bases:

>100_bases
CCCTGACGCGCCGCAAGCCGGTAGCCGGCGGCGGGGTCGCCAAAAGCATGAAACAATGTCTGGTCAGCCGCCCGAGCGGC
GCCATCAGGAGCTCGAAAGC

Downstream 100 bases:

>100_bases
TGGCACGGCACGCTCCCCCCGCCGCCTCGGCCGACGCCGCGCCGGTGGTGCTCGTGACGGGCGCCGCGCGCCGCGCGGGC
CGCGTGTTCGCGCGCCGCTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSKRKTANKTAEPVAAPTTSDIARPSRAEAEDAVRVLLRWAGDDPAREGLLDTPARVVRAYEEFFRGYQLEPREILARTF
SEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFEVLQPK
GVGVILEAAHQCISTRGVHKPGVEMVTSRMLGTFRSDPATRREFLSIVANPSSVNLTNT

Sequences:

>Translated_219_residues
MSKRKTANKTAEPVAAPTTSDIARPSRAEAEDAVRVLLRWAGDDPAREGLLDTPARVVRAYEEFFRGYQLEPREILARTF
SEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFEVLQPK
GVGVILEAAHQCISTRGVHKPGVEMVTSRMLGTFRSDPATRREFLSIVANPSSVNLTNT
>Mature_218_residues
SKRKTANKTAEPVAAPTTSDIARPSRAEAEDAVRVLLRWAGDDPAREGLLDTPARVVRAYEEFFRGYQLEPREILARTFS
EVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFEVLQPKG
VGVILEAAHQCISTRGVHKPGVEMVTSRMLGTFRSDPATRREFLSIVANPSSVNLTNT

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=47.7272727272727, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=47.7272727272727, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI66932970, Length=146, Percent_Identity=43.8356164383562, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=46.3235294117647, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1788476, Length=175, Percent_Identity=37.7142857142857, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=45.1977401129944, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=45, Blast_Score=175, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=45.4545454545455, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=45.4545454545455, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=45.4545454545455, Blast_Score=160, Evalue=5e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24499; Mature: 24368

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRKTANKTAEPVAAPTTSDIARPSRAEAEDAVRVLLRWAGDDPAREGLLDTPARVVRA
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
YEEFFRGYQLEPREILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNH
HHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCHHHCCCEEEECCCC
RVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFEVLQPKGVGVILEAAHQCISTRGVHK
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
PGVEMVTSRMLGTFRSDPATRREFLSIVANPSSVNLTNT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKRKTANKTAEPVAAPTTSDIARPSRAEAEDAVRVLLRWAGDDPAREGLLDTPARVVRA
CCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
YEEFFRGYQLEPREILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNH
HHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCHHHCCCEEEECCCC
RVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFEVLQPKGVGVILEAAHQCISTRGVHK
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
PGVEMVTSRMLGTFRSDPATRREFLSIVANPSSVNLTNT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]