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The map label for this gene is pilR [H]

Identifier: 238023536

GI number: 238023536

Start: 41401

End: 42369

Strand: Reverse

Name: pilR [H]

Synonym: bglu_2g00370

Alternate gene names: 238023536

Gene position: 42369-41401 (Counterclockwise)

Preceding gene: 238023538

Following gene: 238023535

Centisome position: 1.5

GC content: 70.38

Gene sequence:

>969_bases
ATGGCGCGCCCCGATAGCCAACCACCGCGGCTGGCCGGTCAGTCTTCATCGATCGAGGCGCTGCTGGCCAAGGTCAGTCG
CGCCGCATTGACGCAGGCGAGCGTGCTGATCGTCGGCGAGACCGGCTCGGGCAAGGACGTGGTCGCGCGGCTGCTGCACG
CGCTCGGCCCGCGCGCGGCGCAGCCGTTCGTGGCAATCAACTGCGGGGCGGTGGCGCGCGACGTCGCCGAATCGCAGCTG
TTCGGCCATGAGAAGGGCAGCTTCACCGGTGCGGTCGCGCAGCATATCGGCTTTTTCGAAGCCGCGCGCGGCGGCACGCT
GTTTCTCGACGAGATCGCCGACGCGCCGCCCGAATTGCAGGTCAAGCTGCTGCGCGTGCTGGAAAGCGGCACGATCCTGC
GGGTGGGCGGCACCGAGCCGATTCCCGTGGACGTGCGCGTGATCGCGGCCACCCACCACGATCCGGCCGCCGCGGTGCGC
GACGGCCGCTTTCGCGAGGATCTGTTCTACCGCATCGCCACCGTGCCGCTGCACGTGCCGCCGTTGCGCCAGCGCGAAGG
CGACGCGGAGCTGCTCGCGCGCGAACTGGTGGCGCAGCTCAACACGCGCCACGGCACCGCGAAGCGGCTTTCGGCGCAGG
CGCTGCGCGTGCTGAACACGCACAGCTGGCCGGGCAACGTGCGCGAGCTGCGCAATGTGGTGGAGCGCGGCTTCATCCTC
GCCGACGAGCAGATCGAGCTGCATCCGCCGGCGCTGGCACCGGCCGCCGCCAGCGTGCGAGACAACGTGATGACGCTGCA
GGTCGGCGCGACGCTGGCGCAATCGCAGCAGCACTTCATCGCCGCCTCGCTGCAATACTTCGACGGCAACAAGCCGCGCG
CCGCGAAATCGCTCGGCATCAGCCTGAAGACGCTCTACAACCGGCTCGCGCTGATGCGCGACGACGACCCCAAGCCGCCG
GCCGGCTAG

Upstream 100 bases:

>100_bases
TGCATCCATCTCAAAAAAAATGCATGATAAAGGCCTCATTGATGGAATAGCTAGCCCCCTCACATGTGTCCGACCCCGCA
ATCCTCAATTTTCGTTTCCC

Downstream 100 bases:

>100_bases
CCGGCCGGACAGCCCGGTAACGCTTTCCATCCGTTGTAAATCGCGGCAGTCGCCGTGCCGCGTCCGCCGCGCCGGCCCGG
CGAATCGGCGTGGCACGCGC

Product: Fis family protein sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARPDSQPPRLAGQSSSIEALLAKVSRAALTQASVLIVGETGSGKDVVARLLHALGPRAAQPFVAINCGAVARDVAESQL
FGHEKGSFTGAVAQHIGFFEAARGGTLFLDEIADAPPELQVKLLRVLESGTILRVGGTEPIPVDVRVIAATHHDPAAAVR
DGRFREDLFYRIATVPLHVPPLRQREGDAELLARELVAQLNTRHGTAKRLSAQALRVLNTHSWPGNVRELRNVVERGFIL
ADEQIELHPPALAPAAASVRDNVMTLQVGATLAQSQQHFIAASLQYFDGNKPRAAKSLGISLKTLYNRLALMRDDDPKPP
AG

Sequences:

>Translated_322_residues
MARPDSQPPRLAGQSSSIEALLAKVSRAALTQASVLIVGETGSGKDVVARLLHALGPRAAQPFVAINCGAVARDVAESQL
FGHEKGSFTGAVAQHIGFFEAARGGTLFLDEIADAPPELQVKLLRVLESGTILRVGGTEPIPVDVRVIAATHHDPAAAVR
DGRFREDLFYRIATVPLHVPPLRQREGDAELLARELVAQLNTRHGTAKRLSAQALRVLNTHSWPGNVRELRNVVERGFIL
ADEQIELHPPALAPAAASVRDNVMTLQVGATLAQSQQHFIAASLQYFDGNKPRAAKSLGISLKTLYNRLALMRDDDPKPP
AG
>Mature_321_residues
ARPDSQPPRLAGQSSSIEALLAKVSRAALTQASVLIVGETGSGKDVVARLLHALGPRAAQPFVAINCGAVARDVAESQLF
GHEKGSFTGAVAQHIGFFEAARGGTLFLDEIADAPPELQVKLLRVLESGTILRVGGTEPIPVDVRVIAATHHDPAAAVRD
GRFREDLFYRIATVPLHVPPLRQREGDAELLARELVAQLNTRHGTAKRLSAQALRVLNTHSWPGNVRELRNVVERGFILA
DEQIELHPPALAPAAASVRDNVMTLQVGATLAQSQQHFIAASLQYFDGNKPRAAKSLGISLKTLYNRLALMRDDDPKPPA
G

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=301, Percent_Identity=43.5215946843854, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=52.1739130434783, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788550, Length=305, Percent_Identity=40.327868852459, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1789233, Length=315, Percent_Identity=39.0476190476191, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=40.3785488958991, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788905, Length=291, Percent_Identity=41.9243986254296, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1790299, Length=324, Percent_Identity=37.6543209876543, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=41.1949685534591, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=305, Percent_Identity=39.672131147541, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=48.068669527897, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1787583, Length=304, Percent_Identity=36.5131578947368, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081858, Length=286, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=33.8983050847458, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 34545; Mature: 34414

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPDSQPPRLAGQSSSIEALLAKVSRAALTQASVLIVGETGSGKDVVARLLHALGPRAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHCCCCC
QPFVAINCGAVARDVAESQLFGHEKGSFTGAVAQHIGFFEAARGGTLFLDEIADAPPELQ
CCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHH
VKLLRVLESGTILRVGGTEPIPVDVRVIAATHHDPAAAVRDGRFREDLFYRIATVPLHVP
HHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCC
PLRQREGDAELLARELVAQLNTRHGTAKRLSAQALRVLNTHSWPGNVRELRNVVERGFIL
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEE
ADEQIELHPPALAPAAASVRDNVMTLQVGATLAQSQQHFIAASLQYFDGNKPRAAKSLGI
ECCCEEECCCCCCCHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
SLKTLYNRLALMRDDDPKPPAG
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARPDSQPPRLAGQSSSIEALLAKVSRAALTQASVLIVGETGSGKDVVARLLHALGPRAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHCCCCC
QPFVAINCGAVARDVAESQLFGHEKGSFTGAVAQHIGFFEAARGGTLFLDEIADAPPELQ
CCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHH
VKLLRVLESGTILRVGGTEPIPVDVRVIAATHHDPAAAVRDGRFREDLFYRIATVPLHVP
HHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCC
PLRQREGDAELLARELVAQLNTRHGTAKRLSAQALRVLNTHSWPGNVRELRNVVERGFIL
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEE
ADEQIELHPPALAPAAASVRDNVMTLQVGATLAQSQQHFIAASLQYFDGNKPRAAKSLGI
ECCCEEECCCCCCCHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
SLKTLYNRLALMRDDDPKPPAG
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]