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The map label for this gene is priA [H]

Identifier: 237814337

GI number: 237814337

Start: 3963265

End: 3965532

Strand: Reverse

Name: priA [H]

Synonym: GBP346_A4135

Alternate gene names: 237814337

Gene position: 3965532-3963265 (Counterclockwise)

Preceding gene: 237814338

Following gene: 237814334

Centisome position: 96.75

GC content: 72.31

Gene sequence:

>2268_bases
ATGGCTGATGCGTTCGTGCGCGTCGCGCTCGATCATCCGCTGCCGACGCTGTTCGATTACCGGTATCGCGGCGACATGCC
GGCGGCGCCCGGGATGCTCGTGCAGGTGCCGTTCGGCCGGCGCAGCGTGGTCGGGCTCGTATGTGAAGTGACCGCTCACA
CGGACGTGCCGGCGGCCAAGCTGAAGGCCGTCGATGCGGTCTGTACGGAGCTGCCGCCGCTGTCGCGGCACTGGCTCGAA
CTCGCCGCGTTCGCGGCCGACTACTATCAGCGCGGGCTCGGTGAAGTTGCGCTGCCGGCGCTGCCGCAGGCGCTGCGCGA
CGTGCCGCGCTGGGGGCGGCTGCTTGCGCCCGAGCTGCGCTACCGGCTGCTCGACGCCGGGCGCGCCGCGCTGCCGAACG
CGCTGCCCGCGCGCGGCGCGGCGCTGCGCCGGCTCGCGCAGGCGCTCGCCGACGCGCCTGCGCTCTCGCTCGCCGAGGTT
CGCGCGCTGCATCCGAAGGCCGTCGCGGTGCTCGACGAATGGCAGGCGCAAGGCTGGGCCGCGTGCGACGAGATTGCGAT
CACGCATGCCGATGCGCAGCTTGTGGATAACCCGGCACCGGCGTCCGCTCGGCGCCCGACTGCATTGCCGCCGCAACTGA
CCGATCAACAGGCGGAGGCGCTCGACGCGATCGCGGCGGCGGACGGTTTCGCGCCATTCCTGCTGCACGGCGTGACGGGC
AGCGGCAAGACCGAGGTGTATCTGCGCGCGCTCGCCGCGCTGCTCGAGCGGCAGCCCGAAGCACAGGCGCTCGTCCTCGT
GCCCGAGATCAACCTGACGCCGCAGTTCGAAGCGGCGTTTCGCGCGCGCTTCGCCGCGACGCTGCCCGCCGATGCGATCG
TCACGCTGCACAGCGGCCTTGCCGAGGGCGAGCGCGCGCGCCACTGGCTCGCCGCACATACCGGCCGCGCGCGGATCGTG
CTCGGCACGCGGCTCGCGGTGCTCGCGTCGCTGCCGGCGCTTGCGATGATCGTCGTCGACGAGGAGCACGAGCCGGCCTA
CAAGCAACAGGAAGGGTTGCGCTATTCGGCGCGCGATCTCGCCGTGTGGCGGGCGAAGCAGCTCGCGATTCCGGTCGTCC
TCGGCTCGGCGACACCGTCGCTCGAAACCTGGTGGCAGGCCGAGCAGGGCCGCTACCGGCGGCTCACGCTGACGAAGCGC
GCGGTGGCCGACGCGGCGCTGCCGACGGTGCGGCTCGTCGATCTCGAGGAGGAGCGGCGGCGCGGGCGCGGGTCGATGGG
CGGGCTGTCGGGGCCGCTCGTCGCTGCGCTGAAGGCGCGGCTCGAGCGTGGCGAGCAGAGCCTCGTGTTCCTGAACCGGC
GCGGCTATGCGCCCGCGCTCGCGTGCGACGCGTGCGGCTGGGTCGCAGGCTGCCCGCGATGCAGCGCGTATGTCGTGATG
CACAAGCCCGAGCACGCGGTGCGCTGCCATCACTGCGGCTGGGAGGCGCGCATTCCGCGCGCGTGCCCGGAATGCGGGAA
CGTCGATATCGCGCCGCTCGGCCGCGGCACGCAGCGCGTCGAGGAGACGCTCGCGCTCGCCGTGCCGGGCGCGCGGATCC
TGCGCATCGACGCGGACAGCACGCGCCGCAAGGGCAGCGCGCAGGCGCTCTTCTCGGACGTGCACGCGGGCGAGGTCGAC
ATCCTCGTCGGCACGCAGATGATCGCGAAGGGGCACGATTTCCAGCGGGTATCGCTCGTCGGCGTGCTGAATGCGGATAC
CGCATTGTTCTCGCACGATTTTCGCGCAAGCGAGCGGCTATTCGCGCAACTGATGCAGGTGAGCGGCCGCGCGGGGCGCG
CGGGGCTGCCGGGCGAGGTGCTGATCCAGACGCGATATCCGCGTCACGCGCTCTATCACGCGCTCGCGCGCCAGGATTAC
GTGGGGTTCGCCGGCGCGACGCTCGGCGAGCGGCGCGACGCGCACCTGCCGCCGTTCGTCTATCAGGCGCTCTTGCGCGC
CGAGGGGCGCACGCTCGATGCGGCGCTCGCCTTCCTGCAGCAGGCGGCCGACGCGTTCTCGGGGCTGCCGGGCGCGGAGC
GCGTGACGGTGTACGACGCCGTGCCGATGACGATCGTCAAGGTGGCCAATGTCCACCGGGCGCAACTGTTGATCGAGAGC
GCGTCGCGCGCGGCGCTTCAGCACACGCTGCGCGCGTGGCAGCCGCTGCTGCGCGAGCTGAAGGGCGTGCTGCGCTGGAG
CGTCGAGGTCGATCCGCTCGACATCTGA

Upstream 100 bases:

>100_bases
ATCCAAAACTTAGCGCCGAAAGTGACGTTTCACTTTAACTTCACGGGCGTCGCGGATATCCGGCCTGCCGGGCCTGTCGG
CATTCGGCGGTGCCACACCG

Downstream 100 bases:

>100_bases
CGCCGGCGCGTCATTCGTGCGCTTCGCGCAGGCCGGACAGCCGCGCGCGGCGCTCGTCCTTCTTCACGCGTTGCGCACAT
CCGAGCGCCTGGAAAAACAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPNALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERQPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Sequences:

>Translated_755_residues
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPNALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERQPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
>Mature_754_residues
ADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLEL
AAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPNALPARGAALRRLAQALADAPALSLAEVR
ALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTGS
GKTEVYLRALAALLERQPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIVL
GTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRA
VADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVMH
KPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDI
LVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYV
GFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIESA
SRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=41.6337285902503, Blast_Score=492, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81853; Mature: 81722

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPNALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERQPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
ADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPNALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERQPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]