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The map label for this gene is tfdEI [H]

Identifier: 237814225

GI number: 237814225

Start: 3851180

End: 3851872

Strand: Direct

Name: tfdEI [H]

Synonym: GBP346_A4020

Alternate gene names: 237814225

Gene position: 3851180-3851872 (Clockwise)

Preceding gene: 237814221

Following gene: 237814226

Centisome position: 93.96

GC content: 69.12

Gene sequence:

>693_bases
ATGGCTTCGCAATGGATCGACATTCCCGCCGGCAACGACACGTTCGGCGGCTATCTCGCGCTGCCCAAGCGCGGCAAGGG
CCCGGCCATCGTCATCATCCAGGAGATCTTCGGCGTGAACGGCCATATCCGCTCGGTCGCCGACCAGTATGCAGCCGACG
GCTACGTCGCGCTCGCGCCCGACGTGTTCTGGCGCACGCAGCCGCGCATCGAGCTCGGCTACGAAGGCGCGGACCGCGAC
AAGGGCATCGAGCTGTTGCAGAAGACGGACGTCGCGCAGGCGGTCGCCGATATCGGCGCGGCCGCGGCGGCGCTGCGCGC
GCGCCCCGAGGTCGCGGGCAAGCTCGCCGCGATCGGTTATTGCTTCGGCGGACGTCTCGCGTATCTCGCAGCGGCGCGGC
AGCATGTCGACGCCGCGGTCGCCTATTACGGCGGCAGCATCCAGAATCACGTCGACGTCGCGGCGCAGATCGCGCAGCCG
ATCCTCTTCCACTACGCGGGCCACGACCAGTCGATTCCGCTCGACGCCGTCGACAAGGTGAAGGCCGCGTTCGCCGGCCG
CGCGAACGCCGAGTTCCACCTGTATCCGGACGCGCAGCACGGCTTCAACTGCTCGGAGCGCGCATCGTACGACCAGCGCG
CGGCGGCGCTCGCGCACGGCCGCACGCTGACTTTCCTCGCCGAACGCCTGTAA

Upstream 100 bases:

>100_bases
TTCGCGGCGCTGCAACGACGCGGGTTTGATGGCACACTGGGCGCCGCTTCCCCATCCACCGTCAGGACCTTCACCGGTCC
TGCTTCGTCAGGAGAGCAGC

Downstream 100 bases:

>100_bases
GCCGCCGGCGATCGCCGCGCTCGCGCGGCGCGTGGGCGCGCGCCGTGCCGCCGCGCTCGAATTCCGAATCGTTCGCCGCT
CGTCACCACGGGGGGATGAC

Product: dienelactone hydrolase family protein

Products: NA

Alternate protein names: Carboxymethylenebutenolidase I; Dienelactone hydrolase I; DLH I [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MASQWIDIPAGNDTFGGYLALPKRGKGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRIELGYEGADRD
KGIELLQKTDVAQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAARQHVDAAVAYYGGSIQNHVDVAAQIAQP
ILFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL

Sequences:

>Translated_230_residues
MASQWIDIPAGNDTFGGYLALPKRGKGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRIELGYEGADRD
KGIELLQKTDVAQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAARQHVDAAVAYYGGSIQNHVDVAAQIAQP
ILFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
>Mature_229_residues
ASQWIDIPAGNDTFGGYLALPKRGKGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAPDVFWRTQPRIELGYEGADRDK
GIELLQKTDVAQAVADIGAAAAALRARPEVAGKLAAIGYCFGGRLAYLAAARQHVDAAVAYYGGSIQNHVDVAAQIAQPI
LFHYAGHDQSIPLDAVDKVKAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=234, Percent_Identity=34.1880341880342, Blast_Score=120, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24575; Mature: 24444

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQWIDIPAGNDTFGGYLALPKRGKGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAP
CCCCEEECCCCCCCCCCEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHCCCEEEECC
DVFWRTQPRIELGYEGADRDKGIELLQKTDVAQAVADIGAAAAALRARPEVAGKLAAIGY
CHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CFGGRLAYLAAARQHVDAAVAYYGGSIQNHVDVAAQIAQPILFHYAGHDQSIPLDAVDKV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
HHHHCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
ASQWIDIPAGNDTFGGYLALPKRGKGPAIVIIQEIFGVNGHIRSVADQYAADGYVALAP
CCCEEECCCCCCCCCCEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHCCCEEEECC
DVFWRTQPRIELGYEGADRDKGIELLQKTDVAQAVADIGAAAAALRARPEVAGKLAAIGY
CHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CFGGRLAYLAAARQHVDAAVAYYGGSIQNHVDVAAQIAQPILFHYAGHDQSIPLDAVDKV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KAAFAGRANAEFHLYPDAQHGFNCSERASYDQRAAALAHGRTLTFLAERL
HHHHCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]