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The map label for this gene is thiE [H]

Identifier: 237814078

GI number: 237814078

Start: 3712580

End: 3713683

Strand: Reverse

Name: thiE [H]

Synonym: GBP346_A3863

Alternate gene names: 237814078

Gene position: 3713683-3712580 (Counterclockwise)

Preceding gene: 237814079

Following gene: 237814077

Centisome position: 90.61

GC content: 75.09

Gene sequence:

>1104_bases
ATGAGCGCCGCGTTGCCCGACGCGTTCTGGCCGCCCGCCGACGAGCTTACCGAGGCCGCCGAGCGGATTCGCGCGACGCT
CGGTGCGTGGCCGCGGCCGGCCGTGCGCACGCGGATCTGTCTCGCGCCGCCGGAGCAGCCGCGCGCGGCCGACCTGTGGG
TCGCCATCGCGGGCGACGCCGGCGCGCACGCCGCGCACATCGCGCGGCTGAACGCGGCGGGCGCGCAGGCGATCGTCATC
GACGATGCATCGGCGACGCTCCACACGGGCGCGGCGCGCCATGCGCTCGCGTCGCGCGCGCCGCTCGCCGACGACTGGAT
CGCGGCGCTCGCGGCGTTTCTCGATTGCGGCTTCGCCGCGTCCGACGCGCTCGTGCTCGCGCTCGCATGGCGCGACGGCG
ACGAGGCGCGCGGCGGCGATCCGTGGCCCGTCGATCCGGCACGCTTTCCGCGCGTGCTCGGCCTGCCCGCCGCGCCCGAA
CCGGCGTTCGCGCCGTGCCCGCAGCGGCTCGGCCTGTATCCGGTGCTGCCGAGCGCCGAATGGGTCGAGCGCGTGCTCGA
TTGCGGCGTGCGGACCGTGCAACTGCGCGTGAAGGACGCCTCGCCCGACGCGCTGCGCGCGGAGGTCGAGCGGGCCGTTG
CCGCGGGCCGCCGCCATCCGGACGCGCGCGTGTTCATCAACGATCACTGGCGGCTCGCGCTCGACGCGGGCGCATACGGC
GTGCACCTCGGCCAGGAGGATCTGGAGACCGCCGATCTCGGCGCGATCGCGCGGGCGGGCGCGCGGCTCGGCCTGTCGAG
CCACGGGTATTACGAAATGCTCGTCGCGCTGCAGTTCAAGCCGAGCTATCTCGCGCTCGGCCCGGTGTTCGCGACCGCGA
CGAAGGCGGTTGCCGCGCCGCCGCAAGGCCTCGCGCGGCTTGCGCGCTACGTGCGCTTCGCCGGGCCGCAGGCGCCGCTC
GTCGCGATCGGCGGAATCGCGCCCGACACGCTCGGCGCGGTGCTGGCGGCGGGCGTCGGCAGCGCGGCCGTCGTCAGCGC
GATCACGGCGGCGGCCGATTACCGGGAAGCGATTGTTGCGTTGCAGCAAAACTTCGGACGATAA

Upstream 100 bases:

>100_bases
GCGCCGCGTATCTCGCGGGGCCGATGGATGCGCGCGAGAGCGCGCACGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGG
CACCAGGACGGGAGCCACGC

Downstream 100 bases:

>100_bases
TTTGACAATCTCTGACTGGAGCGCCTGAGCGGCCTTTGCGGCATCATTCCGATGCAGGCCCTATAATTCGGCGTTCTGCG
TATAAGGATTGTCCGCTCCG

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSAALPDAFWPPADELTEAAERIRATLGAWPRPAVRTRICLAPPEQPRAADLWVAIAGDAGAHAAHIARLNAAGAQAIVI
DDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPE
PAFAPCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYG
VHLGQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPL
VAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYREAIVALQQNFGR

Sequences:

>Translated_367_residues
MSAALPDAFWPPADELTEAAERIRATLGAWPRPAVRTRICLAPPEQPRAADLWVAIAGDAGAHAAHIARLNAAGAQAIVI
DDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPE
PAFAPCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYG
VHLGQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPL
VAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYREAIVALQQNFGR
>Mature_366_residues
SAALPDAFWPPADELTEAAERIRATLGAWPRPAVRTRICLAPPEQPRAADLWVAIAGDAGAHAAHIARLNAAGAQAIVID
DASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAASDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEP
AFAPCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGV
HLGQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAPPQGLARLARYVRFAGPQAPLV
AIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYREAIVALQQNFGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=208, Percent_Identity=56.25, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38273; Mature: 38142

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALPDAFWPPADELTEAAERIRATLGAWPRPAVRTRICLAPPEQPRAADLWVAIAGDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCEEEEEEECCC
GAHAAHIARLNAAGAQAIVIDDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAA
CCHHHHHHHHCCCCCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAFAPCPQRLGLYPVLPSAE
CCEEEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHH
WVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYG
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCE
VHLGQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAP
EECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCC
PQGLARLARYVRFAGPQAPLVAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYREAIVA
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LQQNFGR
HHHHCCC
>Mature Secondary Structure 
SAALPDAFWPPADELTEAAERIRATLGAWPRPAVRTRICLAPPEQPRAADLWVAIAGDA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCEEEEEEECCC
GAHAAHIARLNAAGAQAIVIDDASATLHTGAARHALASRAPLADDWIAALAAFLDCGFAA
CCHHHHHHHHCCCCCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SDALVLALAWRDGDEARGGDPWPVDPARFPRVLGLPAAPEPAFAPCPQRLGLYPVLPSAE
CCEEEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHH
WVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYG
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCE
VHLGQEDLETADLGAIARAGARLGLSSHGYYEMLVALQFKPSYLALGPVFATATKAVAAP
EECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCC
PQGLARLARYVRFAGPQAPLVAIGGIAPDTLGAVLAAGVGSAAVVSAITAAADYREAIVA
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LQQNFGR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA