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The map label for this gene is proB [H]

Identifier: 237813893

GI number: 237813893

Start: 3540871

End: 3541989

Strand: Reverse

Name: proB [H]

Synonym: GBP346_A3671

Alternate gene names: 237813893

Gene position: 3541989-3540871 (Counterclockwise)

Preceding gene: 237813894

Following gene: 237813892

Centisome position: 86.42

GC content: 68.36

Gene sequence:

>1119_bases
ATGCGTTCGATCATCGCCGATTCGAAGCGCCTGGTAGTGAAAGTGGGCTCGAGCCTCGTCACCAACGACGGCCGCGGGCT
CGATCATGACGCCATCGGCCGATGGGCCGCCCAGATCGCCGCGCTGCGCGGCGCGGGCAAGGAAGTGGTGCTCGTCAGCT
CGGGCGCGATCGCGGAAGGCATGCAGCGCCTCGGTTGGAGCAAGCGGCCGCGCGAGATCGACGAGTTGCAAGCGGCCGCG
GCCGTCGGGCAAATGGGGCTCGCGCAGGTATACGAGAGCCGGTTTGCCGAACACGGGATCCGCACCGCGCAGATTCTCCT
CACGCATGCGGATCTCGCCGACCGCGAACGCTACCTGAACGCGCGCTCGACGCTCCTCACGCTGCTGCGGCTCGGCGTCG
TGCCGATCATCAACGAGAACGACACCGTCGTCACCGACGAAATCAAGTTCGGCGACAACGACACGCTCGGCGCGCTCGTC
GCGAATCTGATCGAAGGCGATACGCTCGTCATCCTGACCGACCAGCCGGGGCTGTTCACGGCCGATCCGCGCAAGGATCC
GGGCGCGACGCTCGTTGCCGAGGCGAGCGCCGGTGCGCCGGAGCTCGAAGCGATGGCGGGCGGCGCGGGCTCGAGCATCG
GCCGCGGCGGCATGCTGACGAAGATTCTCGCGGCCAAGCGCGCCGCGCACAGCGGCGCGAACACCGTGATCGCGAGCGGC
CGCGAGCGCGACGTGCTCGTGCGGCTCGCGGCCGGCGAGGCGATCGGCACGCAACTGATCGCGCGCACCGCGCGGATGGC
GGCGCGCAAGCAATGGATGGCGGACCACCTGCAGGTACGTGGCCATGTCGTTATCGATGCGGGGGCGGTCGACAAGTTGA
CGGCGGGGGGCAAGAGCCTGTTGCCGATCGGCGTCGTCGCCGTGCAGGGCGTGTTCGCGCGCGGCGAAGTGATCGCGTGC
GTCGACGACACCGGCCGTGAAGTCGCGCGCGGGATCACCAATTACAGCAGCGCCGAAACGAAGCTGATCCAGCGCAAGCC
GAGCGGCGAGATCGAAACGGTGCTCGGCTACATGCTCGAGCCCGAGCTGATCCATCGGGACAATCTCGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
CGCCGGCGCGCACTGAGCGTCTCGTCCATCGCCCATGAAGCCGGGCGGCGCGGCCGGCGTACGGGATTCAATCGCGCATC
ACTCAAGGAGACAGCGCACG

Downstream 100 bases:

>100_bases
CGGCGACATGCGTGCCGCGCCTCGGTCGCGGCGGCGAAGGCGACAAAAAAGCCCGCGCATGCGGGCTTTTTGCTTTCGTC
AGCCTGTCGAACCTCGGTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
RERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIAC
VDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
RERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIAC
VDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
RERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIAC
VDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=35.7142857142857, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=35.958904109589, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17569969, Length=278, Percent_Identity=34.8920863309352, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=34.8920863309352, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=423, Percent_Identity=34.9881796690307, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=32.8537170263789, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21357643, Length=352, Percent_Identity=32.1022727272727, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39234; Mature: 39234

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDTLVILTDQPGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEE
ADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
RERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCC
LPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLE
CHHHHHHHHCEEECCCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDTLVILTDQPGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEE
ADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
RERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCC
LPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLE
CHHHHHHHHCEEECCCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA