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The map label for this gene is leuS [H]

Identifier: 237813817

GI number: 237813817

Start: 3471666

End: 3474260

Strand: Reverse

Name: leuS [H]

Synonym: GBP346_A3595

Alternate gene names: 237813817

Gene position: 3474260-3471666 (Counterclockwise)

Preceding gene: 237813818

Following gene: 237813816

Centisome position: 84.77

GC content: 66.74

Gene sequence:

>2595_bases
ATGCACGAAAGATACGTACCCGCCGACGTCGAAGCCGCCGCCCAGAGCGACTGGCGCGCAGCCGATGCCTACCGCTCGAA
GGAAGACGCGAACCGCAAGAAGTTCTACTGCGTGTCGATGCTGCCTTACCCGTCCGGCAAGCTGCACATGGGTCACGTGC
GCAACTACACGATCAACGACGTGATGTACCGCTATCTGCGGATGAACGGCTACAACACGCTGATGCCGATGGGTTGGGAC
GCATTCGGGATGCCGGCCGAGAACGCCGCGATGGCCAACGGCGTGCCGCCCGCGCAGTGGACGTACGAGAACATCGCTTA
CATGAAGAAGCAGATGCAGGCGATGGGCCTCGCGATCGACTGGTCGCGCGAGGTCACGACCTGCAAGCCCGATTACTACA
AGTGGAACCAGTGGCTGTTCCTGAAGATGCTCGAGAAGGGCGTCGCGTACAAGAAGACGGGCACCGTGAACTGGGACCCG
GTCGACCAGACCGTGCTCGCGAACGAGCAGGTGATCGACGGGCGCGGCTGGCGCTCGGGCGCGCTCGTCGAAAAGCGCGA
GATCCCGATGTACTACATGCGGATCACGCAGTACGCGGACGAGCTGCTGAACGATCTCGACGGCCTCGGCTGGCCCGAGC
GCGTGAAGGTGATGCAGCACAACTGGATCGGCAAGAGCTTCGGCGTGAACTTCGGCTTTCCGTACGAGCTCGACGGCGAG
AAGAAGCTGCTGCGCGTGTTCACGACGCGCGCCGACACGATCATGGGCGTCACGTTCTGCGCGATCGCGGCCGAGCATCC
GCTCGCCGCGCGGCTCGCGCGGGACAAGCCCGCGCTGCAGGCGTTCATCGACGAATGCAAGCGCGGCGGCGTCGCCGAGG
CCGACATCGCGACGATGGAGAAGAAGGGCGTCGCCACGGGCTTTTCGGTGTCGCATCCGCTCACGGGCGAGCCCGTCGAG
GTGTGGATCGGCAACTACGTGCTGATGAGCTACGGCGAGGGCGCGGTGATGGGCGTGCCCGCGCACGACGAGCGCGACTT
CGCGTTCGCGAAGAAGTATGGCCTGCCGATCCGGCAGGTGATTGCCGTGGAGGGCGAGACGTACTCGACCGACGCGTGGC
AGGAATGGTACGGCGACAAGACGCGCGCCGTGTGCGTGAACAGCGGCAAGTACGACGGCCTCGCGCACGACGCGGCCGTC
GACGCGATCGCGGCCGAGCTGAAGGCGGGCGGCCTCGGCGACAAGCAGATCACCTACCGGCTGCGCGACTGGGGCATCTC
GCGCCAGCGCTACTGGGGCACGCCGATCCCGATCATCCACTGCCCGTCGTGCGGCGACGTGCCGGTGCCGGAGCAGGACC
TGCCCGTCGTGCTGCCGGAAGACCTCGTGCCGGACGGCACGGGCAACCCGCTCGCGAAGTCCGACGCGTTCCTGAACTGC
ACGTGCCCGAAGTGCGGCGCGGCCGCGAAGCGCGAAACGGACACGATGGACACGTTCGTCGATTCCGCGTGGTACTTCTC
GCGCTACGCGGCGCCCGACGCGCAGACGATGGTCGATGCGCGCACCGACTACTGGATGCCGATGGATCAGTACATCGGCG
GCATCGAGCACGCGATCCTGCACCTGCTGTACTCGCGCTTCTGGGCGAAGGTGATGCGCGATCTCGGTCTCGTCGCGTTC
GGCGAACCGGCGAAGAACCTGCTCACGCAGGGGATGGTGCTCAACGAGACGTTCTACCGCGAGGACGCGGCGGGCAAGAA
GACCTGGTACAACCCGGCCGACGTGACCGTGTCGTTCGACGACAAGGGCCGCCCCGTCGGCGCGGTGCTGAAATCGGACG
GGCAGCCGGTCGAGCTCGGCGGCATCGAGAAGATGTCGAAGTCGAAGAACAACGGCGTCGATCCGCAAATGCTGATCGAC
CATTACGGCGCCGACACCGCGCGCCTTTTCACGATGTTCGCGGCGCCGCCCGAGCAGCAGCTCGAGTGGTCCGGCGCGGG
CGTCGACGGCGCGAGCCGCTTTCTGCGCCGCGTGTGGGCGTTCGGCTTCGCGAACCGCGAAGCGCTGGCCGTGCGCGCGC
CGTTCGACGCCGCGCAACTCGCCGAGGCGGACAAGACGCTGCGCCGCGAGATCCACGGCGTGCTCAAGCAGGCGGATTTC
GATTATCAGCGCTTGCAGTACAACACGGTCGTGTCGGCCGCGATGAAGATGCTCAACGCGATCGAAGGCGCGAAGGGCGC
GACGCCCGCCGTGCTGCGCGAGACTTACGGCGTGCTGCTGCGCGTGCTGTATCCGGTCGTGCCGCACGTCACGTTCGAGC
TGTGGAAGGCGCTCGGCTACGCGGACGAATTCGGCCCGCTGCTCGACGCGCCGTGGCCGAAGGTCGACGAGGCCGCGCTC
GAGCAGGCCGAGATCGAGCTCGTGCTGCAGGTCAACGGCAAGGTGCGCGGCGCGCTGAAGGTCGCGAAGGACGCGAGCCG
CGAGGCGATCGAGGCGGCGGCCGTCGCCGACGGGATGTTCGCGAAATTCGCCGAAGGCAGGCCGGCGAAGAAGATCATTG
TCGTGCCGGGCCGTCTCGTGAACGTCGTCGTTTGA

Upstream 100 bases:

>100_bases
CCCCGGCCGCCGGGGAAGCGCGACTTTCGATCCACTCGAGCGCGCGTGCGCTCGGCACCTTGCGTCGCGCGCCGCTCAAA
AGCCCAGCCCGAACCACACC

Downstream 100 bases:

>100_bases
CGGCGCGCGGCGGGGCTGGCACGCATCAGCAAGGAGCAAAGGTGATCCGCAGATCGTTTTTGATGCTCGTGGGCAGCGCG
GTGCTGCTGTCCGCGTGCGG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MHERYVPADVEAAAQSDWRAADAYRSKEDANRKKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAQWTYENIAYMKKQMQAMGLAIDWSREVTTCKPDYYKWNQWLFLKMLEKGVAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYMRITQYADELLNDLDGLGWPERVKVMQHNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLAARLARDKPALQAFIDECKRGGVAEADIATMEKKGVATGFSVSHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPAHDERDFAFAKKYGLPIRQVIAVEGETYSTDAWQEWYGDKTRAVCVNSGKYDGLAHDAAV
DAIAAELKAGGLGDKQITYRLRDWGISRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGTGNPLAKSDAFLNC
TCPKCGAAAKRETDTMDTFVDSAWYFSRYAAPDAQTMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWAKVMRDLGLVAF
GEPAKNLLTQGMVLNETFYREDAAGKKTWYNPADVTVSFDDKGRPVGAVLKSDGQPVELGGIEKMSKSKNNGVDPQMLID
HYGADTARLFTMFAAPPEQQLEWSGAGVDGASRFLRRVWAFGFANREALAVRAPFDAAQLAEADKTLRREIHGVLKQADF
DYQRLQYNTVVSAAMKMLNAIEGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADGMFAKFAEGRPAKKIIVVPGRLVNVVV

Sequences:

>Translated_864_residues
MHERYVPADVEAAAQSDWRAADAYRSKEDANRKKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAQWTYENIAYMKKQMQAMGLAIDWSREVTTCKPDYYKWNQWLFLKMLEKGVAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYMRITQYADELLNDLDGLGWPERVKVMQHNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLAARLARDKPALQAFIDECKRGGVAEADIATMEKKGVATGFSVSHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPAHDERDFAFAKKYGLPIRQVIAVEGETYSTDAWQEWYGDKTRAVCVNSGKYDGLAHDAAV
DAIAAELKAGGLGDKQITYRLRDWGISRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGTGNPLAKSDAFLNC
TCPKCGAAAKRETDTMDTFVDSAWYFSRYAAPDAQTMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWAKVMRDLGLVAF
GEPAKNLLTQGMVLNETFYREDAAGKKTWYNPADVTVSFDDKGRPVGAVLKSDGQPVELGGIEKMSKSKNNGVDPQMLID
HYGADTARLFTMFAAPPEQQLEWSGAGVDGASRFLRRVWAFGFANREALAVRAPFDAAQLAEADKTLRREIHGVLKQADF
DYQRLQYNTVVSAAMKMLNAIEGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADGMFAKFAEGRPAKKIIVVPGRLVNVVV
>Mature_864_residues
MHERYVPADVEAAAQSDWRAADAYRSKEDANRKKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAQWTYENIAYMKKQMQAMGLAIDWSREVTTCKPDYYKWNQWLFLKMLEKGVAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYMRITQYADELLNDLDGLGWPERVKVMQHNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLAARLARDKPALQAFIDECKRGGVAEADIATMEKKGVATGFSVSHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPAHDERDFAFAKKYGLPIRQVIAVEGETYSTDAWQEWYGDKTRAVCVNSGKYDGLAHDAAV
DAIAAELKAGGLGDKQITYRLRDWGISRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGTGNPLAKSDAFLNC
TCPKCGAAAKRETDTMDTFVDSAWYFSRYAAPDAQTMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWAKVMRDLGLVAF
GEPAKNLLTQGMVLNETFYREDAAGKKTWYNPADVTVSFDDKGRPVGAVLKSDGQPVELGGIEKMSKSKNNGVDPQMLID
HYGADTARLFTMFAAPPEQQLEWSGAGVDGASRFLRRVWAFGFANREALAVRAPFDAAQLAEADKTLRREIHGVLKQADF
DYQRLQYNTVVSAAMKMLNAIEGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADGMFAKFAEGRPAKKIIVVPGRLVNVVV

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=868, Percent_Identity=36.8663594470046, Blast_Score=530, Evalue=1e-150,
Organism=Escherichia coli, GI1786861, Length=873, Percent_Identity=49.2554410080183, Blast_Score=856, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=385, Percent_Identity=23.3766233766234, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71997510, Length=250, Percent_Identity=42, Blast_Score=217, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71997517, Length=250, Percent_Identity=42, Blast_Score=217, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI212645227, Length=352, Percent_Identity=30.3977272727273, Blast_Score=146, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323414, Length=831, Percent_Identity=39.8315282791817, Blast_Score=563, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6321531, Length=663, Percent_Identity=23.2277526395173, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355409, Length=893, Percent_Identity=34.4904815229563, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281366294, Length=355, Percent_Identity=22.5352112676056, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96136; Mature: 96136

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERYVPADVEAAAQSDWRAADAYRSKEDANRKKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAQWTYENIAYMKKQMQAMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEE
WSREVTTCKPDYYKWNQWLFLKMLEKGVAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYMRITQYADELLNDLDGLGWPERVKVMQHNWIGKSFGVNFGFPYELDGE
CCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCCCEEECCH
KKLLRVFTTRADTIMGVTFCAIAAEHPLAARLARDKPALQAFIDECKRGGVAEADIATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVSHPLTGEPVEVWIGNYVLMSYGEGAVMGVPAHDERDFAFAKKYGLPIRQV
HCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCHHHHHHHCCCHHHE
IAVEGETYSTDAWQEWYGDKTRAVCVNSGKYDGLAHDAAVDAIAAELKAGGLGDKQITYR
EEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHEEEE
LRDWGISRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGTGNPLAKSDAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCCEEEE
TCPKCGAAAKRETDTMDTFVDSAWYFSRYAAPDAQTMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWAKVMRDLGLVAFGEPAKNLLTQGMVLNETFYREDAAGKKTWYNPADVTVSFD
HHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEC
DKGRPVGAVLKSDGQPVELGGIEKMSKSKNNGVDPQMLIDHYGADTARLFTMFAAPPEQQ
CCCCCEEHHCCCCCCEEECCCCHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHH
LEWSGAGVDGASRFLRRVWAFGFANREALAVRAPFDAAQLAEADKTLRREIHGVLKQADF
CEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIEGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKALGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGPLLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADGMF
CHHHCCCCCCCCCCCCHHHHHHCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AKFAEGRPAKKIIVVPGRLVNVVV
HHHHCCCCCCEEEEECCCEEEEEC
>Mature Secondary Structure
MHERYVPADVEAAAQSDWRAADAYRSKEDANRKKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAQWTYENIAYMKKQMQAMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEE
WSREVTTCKPDYYKWNQWLFLKMLEKGVAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYMRITQYADELLNDLDGLGWPERVKVMQHNWIGKSFGVNFGFPYELDGE
CCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCCCEEECCH
KKLLRVFTTRADTIMGVTFCAIAAEHPLAARLARDKPALQAFIDECKRGGVAEADIATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVSHPLTGEPVEVWIGNYVLMSYGEGAVMGVPAHDERDFAFAKKYGLPIRQV
HCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCHHHHHHHCCCHHHE
IAVEGETYSTDAWQEWYGDKTRAVCVNSGKYDGLAHDAAVDAIAAELKAGGLGDKQITYR
EEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHEEEE
LRDWGISRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGTGNPLAKSDAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCCEEEE
TCPKCGAAAKRETDTMDTFVDSAWYFSRYAAPDAQTMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWAKVMRDLGLVAFGEPAKNLLTQGMVLNETFYREDAAGKKTWYNPADVTVSFD
HHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEC
DKGRPVGAVLKSDGQPVELGGIEKMSKSKNNGVDPQMLIDHYGADTARLFTMFAAPPEQQ
CCCCCEEHHCCCCCCEEECCCCHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHH
LEWSGAGVDGASRFLRRVWAFGFANREALAVRAPFDAAQLAEADKTLRREIHGVLKQADF
CEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIEGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKALGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGPLLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADGMF
CHHHCCCCCCCCCCCCHHHHHHCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AKFAEGRPAKKIIVVPGRLVNVVV
HHHHCCCCCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA