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The map label for this gene is ytwF [H]

Identifier: 237813468

GI number: 237813468

Start: 3131356

End: 3131685

Strand: Direct

Name: ytwF [H]

Synonym: GBP346_A3244

Alternate gene names: 237813468

Gene position: 3131356-3131685 (Clockwise)

Preceding gene: 237813467

Following gene: 237813471

Centisome position: 76.4

GC content: 68.79

Gene sequence:

>330_bases
GTGCCGATGCAGATCCTGAGCGCTTCCGCCCTCGCCGAATGGCTGCGCGACCCGGCGCGCCCGGCGCCCATCGTGCTCGA
CGTGCGCGAGCCGTGGGAGATCGCGACCGCGAAGATCGACGGCAGCGTGTCGATCCCGATGCAGCAGATTCCCGCGCGCA
GCGAGGAGCTCGACGACGAAGCGGCAATCGTCTGCGTGTGCCATCACGGGATGCGCAGCGCGCAGGTCGCGATGTTCCTC
GAATCGCGCGGCTTCACGAAGCTCTACAACCTGCAAGGCGGGATCGACGCGTGGTCGCGCGACGTCGATCCCGCGGTGCC
GCGCTACTGA

Upstream 100 bases:

>100_bases
TCGACGACACGCAATATCGCGTCGCCGACGTATTCGAGACCTACGTCGATCACCTCGTCAACGCGATCGAGCCGTCGCGC
TTCAAGTTCTGAGGCCCCGC

Downstream 100 bases:

>100_bases
GCGCGCATCGCGGCGGCGCGCGCGACGCGCGCCGCTTCGCCCGGGCGCGCCGCCCGTTTCCCTGAGCGCGGCGCCCGTTT
GCCGCGCTCCGCCTACTCCG

Product: rhodanese domain protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPAVPRY

Sequences:

>Translated_109_residues
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPAVPRY
>Mature_108_residues
PMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFLE
SRGFTKLYNLQGGIDAWSRDVDPAVPRY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 12076; Mature: 11945

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDE
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEECCHHHCCCCCCCCCCC
AAIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPAVPRY
CEEEEEECCCCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDE
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEECCHHHCCCCCCCCCCC
AAIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPAVPRY
CEEEEEECCCCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]