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The map label for this gene is citH [H]

Identifier: 237813337

GI number: 237813337

Start: 3016543

End: 3017895

Strand: Reverse

Name: citH [H]

Synonym: GBP346_A3106

Alternate gene names: 237813337

Gene position: 3017895-3016543 (Counterclockwise)

Preceding gene: 237813338

Following gene: 237813334

Centisome position: 73.63

GC content: 66.37

Gene sequence:

>1353_bases
GTGACACGCGCCGTGCGCCGCCCCCGGCGGTCGCGGCGCAGCCTTATCGAGAGAGACATGTCTACCGCGTCCCCCGCAAT
CCAGCAGGAATCCAAAGCCAGGACCGTGTTCCGCGTCGTCAGCGGCAACTTCCTGGAGATGTACGACTTCATGGTCTACG
GCTACTACGCGTCCGCGATCGCCAAGACCTACTTCCCGAGCGGCAACGCGTTCGCCTCGCTGATGCTGTCGCTGTCGGTG
TTCGGCGCGGGCTTTCTGATGCGTCCCGTCGGCGCGATCGTGCTCGGCGCGTACATCGACCATCACGGCCGCCGCAAGGG
CCTCATCCTGACGCTCGCGTTGATGGCGCTCGGCACGCTGACCGTCGCGACGATTCCGGGCTACGCGACGATCGGCGTGC
TCGCGCCGATTCTCGTGCTGCTCGGCCGCCTGCTGCAGGGTTTCTCGGCGGGCGTCGAGCTGGGCGGCGTATCGGTGTAT
CTGTCCGAGATCGCGACGAAGGGCAACAAGGGCTTCTATACGTCGTGGCAGTCGGGCAGCCAGCAGGTGGCCGTGGTGTT
CGCCGCGTTCGTCGGCGTGCTGCTGAACCGCGCGCTGCCTGTCGAGCAAATGACGTCGTGGGGCTGGCGCATCCCGTTCC
TGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGCTGAAGGAGACCGACGAGTTCCTCGCGAAGCGC
CGCCGCCCGAGCATGGGCGAGATCATGAAGTCGATGCTCGAGAACTGGGGCGTGGTGCTGGCCGGCATGGGGATGGTCAT
CATGACGACCGTGTCGTTCTACATGATCACCGCGTACACGCCGACTTTCGGCAAGGAAGTGCTGCATCTGTCGGCGATCG
ACGCGCTCGTCGTGACCGTTTGCGTCGGGCTGTCGAACCTGGTCTGGCTGCCGCTGTCGGGCGCGCTGTCCGATCGGATC
GGCCGCCGCCCGGTGCTGATCGCGTTCACCGCGCTCACGATCCTCACCGCGTATCCGGCGATGCAGTGGCTCGTCGGTTC
GCCGTCGTTCCTGCGGCTGTTGGGCGTCGAGCTGTGGCTGTCGTTCCTGTACGGCTCGTACAACGGCGCGATGGTCGTCG
CGCTGACCGAGGTGATGCCGGCCGACGTGCGCACCGCGGGCTTCTCGCTCGCTTACAGCCTCGCGACGACGATCGGCGGC
TTCACGCCGGCGATCTCGACGCTGCTGATTCACGAGACCGGCAACAAGGCGGCGCCGGGCCTCTGGCTCGGCCTCGCCGC
GATCTGCGGGCTGATCGCGACGCTCGTGCTGTACCGGTCGCCTGAAGCGCGCAATCAATACAAGACGACTTGA

Upstream 100 bases:

>100_bases
CATTGACGCATCCGGCTGTCGTTCGCAGTAAGATGGCGGACGCCGCGCAGCGCACCATCGGTCTGCGCGGGCCCCGGCGC
GCCGGGGCCAGAAACACGAT

Downstream 100 bases:

>100_bases
GCGAACCGGCCGCACGCGGCCCAGCAGGCCGCCGTCGACGAATCGACAAACGGAGCGCGTCGCGCTCCGTTTGGGTTGGG
GCGAAGCGTCGGCGCGGCGC

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTRAVRRPRRSRRSLIERDMSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSV
FGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVY
LSEIATKGNKGFYTSWQSGSQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKR
RRPSMGEIMKSMLENWGVVLAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRI
GRRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGG
FTPAISTLLIHETGNKAAPGLWLGLAAICGLIATLVLYRSPEARNQYKTT

Sequences:

>Translated_450_residues
MTRAVRRPRRSRRSLIERDMSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSV
FGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVY
LSEIATKGNKGFYTSWQSGSQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKR
RRPSMGEIMKSMLENWGVVLAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRI
GRRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGG
FTPAISTLLIHETGNKAAPGLWLGLAAICGLIATLVLYRSPEARNQYKTT
>Mature_449_residues
TRAVRRPRRSRRSLIERDMSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVF
GAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYL
SEIATKGNKGFYTSWQSGSQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRR
RPSMGEIMKSMLENWGVVLAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRIG
RRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGF
TPAISTLLIHETGNKAAPGLWLGLAAICGLIATLVLYRSPEARNQYKTT

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=30.5239179954442, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=33.1786542923434, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=28.8782816229117, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789941, Length=425, Percent_Identity=31.2941176470588, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082231, Length=162, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48863; Mature: 48732

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TRAVRRPRRSRRSLIERDMSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAI
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCC
QQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKR
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRPSMGEIMKSMLENWGVVLAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHH
CVGLSNLVWLPLSGALSDRIGRRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLGVELWL
HHHHHHHEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETGNKAAPG
HHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCCCH
LWLGLAAICGLIATLVLYRSPEARNQYKTT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]