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The map label for this gene is pcpB [H]

Identifier: 237813334

GI number: 237813334

Start: 3012510

End: 3014156

Strand: Reverse

Name: pcpB [H]

Synonym: GBP346_A3103

Alternate gene names: 237813334

Gene position: 3014156-3012510 (Counterclockwise)

Preceding gene: 237813337

Following gene: 237813328

Centisome position: 73.54

GC content: 72.19

Gene sequence:

>1647_bases
ATGGCAGTCGTTCCGCTCTCCACGCCTCCCGTGCTGATCGTCGGCGCCGGTCCGACGGGGCTCGCCGCCGCGCTTTGTCT
CGCGCGCGCCCGCGTGCCGGTGCGTATCGTCGACAAGGCGGCCCGACCCGCGCGTTACTCGCGCGCGATCGGCATTCAGG
CCAGAACGCTCGAGCTCCTCGAGCAGCAGCGCGTCGTCGACCGGTTCGTCGAGCTCGGCCATCGCGCGCGGCTCGCGATC
CTGTACTCGGCCGGCCAGCGCATCGCCGAGCTCGACTTCGATCCGCTGCAGACGCGCTATCCGTACCTGCTGTTTCTCGA
CCAGACCGTCACCGAGCGTTTGCTGACCGAGCATCTCGCGACGTTCGACGTGCACGTCGAGCGCGGCGTGACGCTCACGC
ATTGCGCGCAGCCGGAAGGCGCGCTCGACGTGCGGCTGCGCCACGGCGACGGCCGCGACGAGCGTCTGCAGCCGTCATAC
GTCGTCGCGGCGGACGGCGCGCGCAGCACGGTCCGGCATTTGCTCGACGTCGATTTCGTCGGGCATGCGTTCGAGCAGAC
GTTCATGCTCGCCGATCTCGAGCTCGGCGCCGACTGGCCCGACGACGAGATCCATCTGTTCACGACGGGCGAGGGCCTCG
CCGGGCTCTTCCCGATGGGCGGCGGCCGCTACCGGCTCGTCGCCGATCGCCCGCCGCGCAACGGCGCGCTCGACGACGAG
CGCGGGCCGTCGCTCGAGCTGTGCCGCGAAGTCGTGCGCGCGCGCGTGACGCCGGATCTGAAGGTCGGCGATCTCGCGTG
GTCGTCGTATTTTCATCTGCACAGCCGAATGGTCGCGCGGCTGCGCGTCGGGCGCGTGTTCTTCGCGGGCGACGCGGCGC
ACGTGCACAGCCCGGCGGGCGCGCAGGGGATGAACACGGGTATCCAGGAGGCGTTCAATCTCGGCTGGAAGCTCGCGCGC
GTGCTGGCGGGCAACGCCCCCGAGCGGCTGCTCGACACGTATCACGCGGAGCGCCATCCGATCGAGCGCGACGTGCTGCG
CCAGACGAGCTTCGCGACGCAGGTCGTCGAGGCGGATCGCGGGCCGCTCAAGCTGCTGCGCGATCACATCGTGCCGATCC
TCGCGTCGTTCGGGCCGCTGCGCGATGCGGCGCGCCGCACGGTCAGCGAACTCGCGATCCAGTATCGGAAGAGTCCGCTC
ACGCTCGAGCGCGTGCTCGACGGCGGGCCGCGCGCGGGCGAGCGCGCGCCGGACGCGCTCGTGCACGTGCTCGACGGGCC
GCTTGGCAAGGCGCCGGGCGTCGCGCGCCTCTTCGATCTGCACGACCCCGCGCATTTCACGCTGCTCGTGCTCGAGCCGC
GCGAGGCGATCGACGACACGCTGCCGTCGGACCCCGGCGAGGACGGCCGCGCGCTCGCCGGCGCGCTCGAGCGGATCATG
CCGGGCGCGGTGCGCTGCTGGTGCGCGACGGACGCGGAAGGCGAGGGCGCGCCGCTGCTGGCCGATGCGTACGGGCGCGC
GCGGCCCGTGTTCTATCTGCTGCGCCCGGACGGCTACGTCGCCGCGCGCGGGCGCCCGGCGACCGACGCGAACGCGCTGC
TGCGCCATTGCGAGACGTGGTTTTCGGGGATGCGGCTGGAGACTTGA

Upstream 100 bases:

>100_bases
CCGAATCGGTCAACCGCCCGCCCGGCCACTCGGCCACCCGCTATGGATGCGGCGCGGCGGCGGGCGTAGACTGCGAATGT
TGCGTATCGACGAGGTCCTT

Downstream 100 bases:

>100_bases
AAGCGGCCGCGCTCGCCGGGCGCGGGCGGCTGCGCGTCGGGCGGCGCCATCGTGGGTTGCGTGCGGCGCCGCTGGCGTAT
GTGCGGGCGAGCCGTGCCGT

Product: FAD-dependent oxidoreductase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAVVPLSTPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPARYSRAIGIQARTLELLEQQRVVDRFVELGHRARLAI
LYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCAQPEGALDVRLRHGDGRDERLQPSY
VVAADGARSTVRHLLDVDFVGHAFEQTFMLADLELGADWPDDEIHLFTTGEGLAGLFPMGGGRYRLVADRPPRNGALDDE
RGPSLELCREVVRARVTPDLKVGDLAWSSYFHLHSRMVARLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLAGNAPERLLDTYHAERHPIERDVLRQTSFATQVVEADRGPLKLLRDHIVPILASFGPLRDAARRTVSELAIQYRKSPL
TLERVLDGGPRAGERAPDALVHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALAGALERIM
PGAVRCWCATDAEGEGAPLLADAYGRARPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLET

Sequences:

>Translated_548_residues
MAVVPLSTPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPARYSRAIGIQARTLELLEQQRVVDRFVELGHRARLAI
LYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCAQPEGALDVRLRHGDGRDERLQPSY
VVAADGARSTVRHLLDVDFVGHAFEQTFMLADLELGADWPDDEIHLFTTGEGLAGLFPMGGGRYRLVADRPPRNGALDDE
RGPSLELCREVVRARVTPDLKVGDLAWSSYFHLHSRMVARLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLAGNAPERLLDTYHAERHPIERDVLRQTSFATQVVEADRGPLKLLRDHIVPILASFGPLRDAARRTVSELAIQYRKSPL
TLERVLDGGPRAGERAPDALVHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALAGALERIM
PGAVRCWCATDAEGEGAPLLADAYGRARPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLET
>Mature_547_residues
AVVPLSTPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPARYSRAIGIQARTLELLEQQRVVDRFVELGHRARLAIL
YSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCAQPEGALDVRLRHGDGRDERLQPSYV
VAADGARSTVRHLLDVDFVGHAFEQTFMLADLELGADWPDDEIHLFTTGEGLAGLFPMGGGRYRLVADRPPRNGALDDER
GPSLELCREVVRARVTPDLKVGDLAWSSYFHLHSRMVARLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARV
LAGNAPERLLDTYHAERHPIERDVLRQTSFATQVVEADRGPLKLLRDHIVPILASFGPLRDAARRTVSELAIQYRKSPLT
LERVLDGGPRAGERAPDALVHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALAGALERIMP
GAVRCWCATDAEGEGAPLLADAYGRARPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLET

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=542, Percent_Identity=25.2767527675277, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 60130; Mature: 59999

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPLSTPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPARYSRAIGIQARTLELL
CEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHCCHHHHHHHH
EQQRVVDRFVELGHRARLAILYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLA
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFDVHVERGVTLTHCAQPEGALDVRLRHGDGRDERLQPSYVVAADGARSTVRHLLDVDFV
HHEEECCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
GHAFEQTFMLADLELGADWPDDEIHLFTTGEGLAGLFPMGGGRYRLVADRPPRNGALDDE
HHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCC
RGPSLELCREVVRARVTPDLKVGDLAWSSYFHLHSRMVARLRVGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLAGNAPERLLDTYHAERHPIERDVLRQTSFATQVVEADR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
GPLKLLRDHIVPILASFGPLRDAARRTVSELAIQYRKSPLTLERVLDGGPRAGERAPDAL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALAGALERIM
HHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHC
PGAVRCWCATDAEGEGAPLLADAYGRARPVFYLLRPDGYVAARGRPATDANALLRHCETW
CCCEEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
FSGMRLET
HCCCCCCC
>Mature Secondary Structure 
AVVPLSTPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPARYSRAIGIQARTLELL
EEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHCCHHHHHHHH
EQQRVVDRFVELGHRARLAILYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLA
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFDVHVERGVTLTHCAQPEGALDVRLRHGDGRDERLQPSYVVAADGARSTVRHLLDVDFV
HHEEECCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
GHAFEQTFMLADLELGADWPDDEIHLFTTGEGLAGLFPMGGGRYRLVADRPPRNGALDDE
HHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCC
RGPSLELCREVVRARVTPDLKVGDLAWSSYFHLHSRMVARLRVGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLAGNAPERLLDTYHAERHPIERDVLRQTSFATQVVEADR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
GPLKLLRDHIVPILASFGPLRDAARRTVSELAIQYRKSPLTLERVLDGGPRAGERAPDAL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALAGALERIM
HHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHC
PGAVRCWCATDAEGEGAPLLADAYGRARPVFYLLRPDGYVAARGRPATDANALLRHCETW
CCCEEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
FSGMRLET
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]