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The map label for this gene is mltB [H]

Identifier: 237813286

GI number: 237813286

Start: 2966913

End: 2968202

Strand: Direct

Name: mltB [H]

Synonym: GBP346_A3053

Alternate gene names: 237813286

Gene position: 2966913-2968202 (Clockwise)

Preceding gene: 237813284

Following gene: 237813302

Centisome position: 72.39

GC content: 66.98

Gene sequence:

>1290_bases
ATGATCTTCCACCAGCCTGCCGCACCCGCCTCGCTCGCTTTCCGTCTTCGCTTCCCGCTCGCCGCGCTGTCGCTCGCCGC
GACGCTCTTCGCGTCGGCCGCCGTCGCGCAAACGCAGCCCGCCGCAGCCGTCGTCGCGCAAGAGCCGGCGCAATCGCAAT
CGCAATTGCAATTGCAGCCGCAGCCGCAGCCGCAGCCGCAGCCGCAGCCGAGCGTACAGCAGGGGCAAACATTCGAAGAA
GAAATCATTCCGCAGCGCTACGCGAACAACGCGAAGATCGACGCGTTCATCGCCGACATGGTCGCGCGCCACGACTTCGA
TGCGAACGCGCTGCATGCGCTGTTCGCGCGCGTGAGCTACTCGGCGACCGCCGCGAAGCTCGTGATGCCCGCGCCGTCGC
CAGCCGTCAAGAACTGGCGCGTCTACCAGTCGCGCTTTCTCGACGCGGTGCGCGTGAACGCCGGCGTGAAATTCTGGCGC
GCGAACCAAGGCACGCTGCAACGCGCGTCCACGGAATTCGGCGTGCCGCCCGAGGTGATCGTCGGCATCATCGGCGTCGA
GACGATCTACGGCCGCTACATGGGCAACTTCCGCACGCTCGACGCGCTGACGACGCTCGCGTTCGACTATCCGAACACGC
CGAATCGCGACGCGCGGCAGGCGACGTTTCGCAAGAATCTCGAAGATTTCCTCGTCTGGACGCGCGACAGCCAGCTCGAC
CCGACGGGCGTGCTCGGCTCGTACACGGGCGCGGTCGGCATCCCGCAGTTCCTGCCGAGCAGCATCCGCGATTACGCGGT
CGACTACGACGGCAATGGTCACATCGATCTGCGCGCGAGCCAGGCCGATGCGATCGGCAGCGTCGCGAACTATCTGAAGC
AGCACGGCTGGGAAACCGGTCGGCCGGTAGTATGGAACATCGCGCCCGATACGGGCAGCCAGGGCGTCGCGCAAGCGGCG
GCGGACGGTCGCCCCGAGCCGCACTGGGTGCTGTCGCAGTTGCTGCGCGCGGGGCTCGTGCTCGACGAGCCGTCCGTGAA
CATCGCATCGGAGGCGAGCACGCCCGTCACCGTGGTCGATTTGCCGACGCCCGGCCGCGCAACCGAATACAAGCTCGGAC
TGCAGAATTTCTACGTGCTGACCCGCTACAACCGCAGCTTCTTCTATGCGCTCGCCGTCTATCAGCTGGGTGAGCGTGTG
AAGGCGCAGATGGAAGCGAGCGGCGCGTTGACACCGTCGCCCGCCGACGCGGCAACCGGCTCACCCGCCGCACAGCCGCC
GTCCGAATGA

Upstream 100 bases:

>100_bases
CGCCGCTAACTTACCACATCACGCCCCCCGCCGGGCCGGCGCGACGGGCGTGTCGGTTATACTGCCCCGAAATCGTCCGT
CTCGTCCGCTTCGCTTCGAC

Downstream 100 bases:

>100_bases
CGCCGACGCGGCGCGAAGCGAGCGATGGCTTGAACGAAGGCTGAATGAAAAGCGCCGCGTCAGCGGCGCTTCAGACTGCT
GACGAAACCCACGTTTTTCG

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MIFHQPAAPASLAFRLRFPLAALSLAATLFASAAVAQTQPAAAVVAQEPAQSQSQLQLQPQPQPQPQPQPSVQQGQTFEE
EIIPQRYANNAKIDAFIADMVARHDFDANALHALFARVSYSATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAGVKFWR
ANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNRDARQATFRKNLEDFLVWTRDSQLD
PTGVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASQADAIGSVANYLKQHGWETGRPVVWNIAPDTGSQGVAQAA
ADGRPEPHWVLSQLLRAGLVLDEPSVNIASEASTPVTVVDLPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQLGERV
KAQMEASGALTPSPADAATGSPAAQPPSE

Sequences:

>Translated_429_residues
MIFHQPAAPASLAFRLRFPLAALSLAATLFASAAVAQTQPAAAVVAQEPAQSQSQLQLQPQPQPQPQPQPSVQQGQTFEE
EIIPQRYANNAKIDAFIADMVARHDFDANALHALFARVSYSATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAGVKFWR
ANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNRDARQATFRKNLEDFLVWTRDSQLD
PTGVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASQADAIGSVANYLKQHGWETGRPVVWNIAPDTGSQGVAQAA
ADGRPEPHWVLSQLLRAGLVLDEPSVNIASEASTPVTVVDLPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQLGERV
KAQMEASGALTPSPADAATGSPAAQPPSE
>Mature_429_residues
MIFHQPAAPASLAFRLRFPLAALSLAATLFASAAVAQTQPAAAVVAQEPAQSQSQLQLQPQPQPQPQPQPSVQQGQTFEE
EIIPQRYANNAKIDAFIADMVARHDFDANALHALFARVSYSATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAGVKFWR
ANQGTLQRASTEFGVPPEVIVGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNRDARQATFRKNLEDFLVWTRDSQLD
PTGVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASQADAIGSVANYLKQHGWETGRPVVWNIAPDTGSQGVAQAA
ADGRPEPHWVLSQLLRAGLVLDEPSVNIASEASTPVTVVDLPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQLGERV
KAQMEASGALTPSPADAATGSPAAQPPSE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=325, Percent_Identity=40.6153846153846, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46464; Mature: 46464

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFHQPAAPASLAFRLRFPLAALSLAATLFASAAVAQTQPAAAVVAQEPAQSQSQLQLQP
CCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCEEECC
QPQPQPQPQPSVQQGQTFEEEIIPQRYANNAKIDAFIADMVARHDFDANALHALFARVSY
CCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
SATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAGVKFWRANQGTLQRASTEFGVPPEVI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCHHHH
VGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNRDARQATFRKNLEDFLVWTRDSQLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHEEECCCCCCC
PTGVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASQADAIGSVANYLKQHGWETG
CCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
RPVVWNIAPDTGSQGVAQAAADGRPEPHWVLSQLLRAGLVLDEPSVNIASEASTPVTVVD
CCEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEE
LPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQLGERVKAQMEASGALTPSPADAATG
CCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SPAAQPPSE
CCCCCCCCC
>Mature Secondary Structure
MIFHQPAAPASLAFRLRFPLAALSLAATLFASAAVAQTQPAAAVVAQEPAQSQSQLQLQP
CCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCEEECC
QPQPQPQPQPSVQQGQTFEEEIIPQRYANNAKIDAFIADMVARHDFDANALHALFARVSY
CCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
SATAAKLVMPAPSPAVKNWRVYQSRFLDAVRVNAGVKFWRANQGTLQRASTEFGVPPEVI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCHHHH
VGIIGVETIYGRYMGNFRTLDALTTLAFDYPNTPNRDARQATFRKNLEDFLVWTRDSQLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHEEECCCCCCC
PTGVLGSYTGAVGIPQFLPSSIRDYAVDYDGNGHIDLRASQADAIGSVANYLKQHGWETG
CCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
RPVVWNIAPDTGSQGVAQAAADGRPEPHWVLSQLLRAGLVLDEPSVNIASEASTPVTVVD
CCEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEE
LPTPGRATEYKLGLQNFYVLTRYNRSFFYALAVYQLGERVKAQMEASGALTPSPADAATG
CCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SPAAQPPSE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]