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The map label for this gene is folD [H]

Identifier: 237813036

GI number: 237813036

Start: 2726493

End: 2727350

Strand: Direct

Name: folD [H]

Synonym: GBP346_A2796

Alternate gene names: 237813036

Gene position: 2726493-2727350 (Clockwise)

Preceding gene: 237813035

Following gene: 237813038

Centisome position: 66.52

GC content: 69.23

Gene sequence:

>858_bases
ATGACAGCCACCCTGATCGACGGCAACGCCCTTTCGAAGACCCTGCGCGCGCAAGCGGCCGAGCGCGCCGCCGCGCTCGC
CGCGCGCGGCCACCGGCCGGGCCTCGCGGTGATCCTCGTCGGCGACAACCCGGCGAGCGAAGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCTGAAGGACCGCTATCCGGCGACGCTCTCCGAGCCCGAGCTGCTCGCG
CGCATCGACGAACTGAACCGCGACCCGAAGATCCACGGCATCCTCGTGCAGTTGCCGCTGCCCGCGCACATCGACAGCCA
CAAGGTGATCGAGGCGATCGCGCCGGAGAAGGACGTCGACGGCTTTCACGTCGCGAACGCCGGCGCGCTGCTGACGGGCA
AGCCGCTGTTCCGCCCGTGCACGCCGTACGGCGTGATGAAAATGTTCGAGGCGTACAAGATTCCGCTGCAGGGCGCGAAC
GCGGTCGTGATCGGCCGCTCGAACATCGTGGGCAAGCCGATGGCGCTCCTGCTGCTCGAGGCGGGCGCGACCGTCACGAT
CTGCCACAGCAAGACCCGCGAGCTCGCCGCGCACACGCGCGCGGCCGACATCGTCGTCGCGGCGGTCGGCAAGCGCAACG
TGCTGACGGCCGACATGGTGAAGCCGGGCGCGACCGTGATCGACGTCGGGATGAACCGCAACGACGAAGGCAAGCTGTGC
GGCGATGTCGACTTCGCGGGCGTCTCGCAGGTCGCGGGCCACATCACGCCCGTGCCGGGCGGCGTCGGCCCGATGACGAT
CACGATGCTGCTCGTCAACACGATCGAAGCCGCCGAGCGCGCCGCGGCCACGGCCTGA

Upstream 100 bases:

>100_bases
TCGGCTGACGACAGGCGGGCGGTATAATTCGATGCTTCGCTGCAGCGCAACCTGGCGCCGCACGCCCGCATCTCCAACCT
TGCCCAAGCGAATTCCCACC

Downstream 100 bases:

>100_bases
CGGCGGATTCGCCGACGCGGCGAGAAAGCGGCGGCGACATCGGCGGCGGGCCGGCCGGATTCGGACGGGAATAGCCGCAA
GCAGCGGCACGGCCATCGCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTATLIDGNALSKTLRAQAAERAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGAN
AVVIGRSNIVGKPMALLLLEAGATVTICHSKTRELAAHTRAADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAATA

Sequences:

>Translated_285_residues
MTATLIDGNALSKTLRAQAAERAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGAN
AVVIGRSNIVGKPMALLLLEAGATVTICHSKTRELAAHTRAADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAATA
>Mature_284_residues
TATLIDGNALSKTLRAQAAERAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLAR
IDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANA
VVIGRSNIVGKPMALLLLEAGATVTICHSKTRELAAHTRAADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLCG
DVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAATA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=48.2993197278912, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.2585034013605, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.0689655172414, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=28.643216080402, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=52.6881720430108, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=41.0169491525424, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=46.8531468531469, Blast_Score=258, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=42, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=293, Percent_Identity=26.6211604095563, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=50.3401360544218, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=50.3401360544218, Blast_Score=264, Evalue=5e-71,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=8e-68,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29956; Mature: 29825

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLIDGNALSKTLRAQAAERAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEECCC
GFFSLKDRYPATLSEPELLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANAVVIGRSNIVGKPMALLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
AGATVTICHSKTRELAAHTRAADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
CCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCCCEE
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAATA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATLIDGNALSKTLRAQAAERAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEECCC
GFFSLKDRYPATLSEPELLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANAVVIGRSNIVGKPMALLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
AGATVTICHSKTRELAAHTRAADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
CCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCCCEE
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAATA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA