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The map label for this gene is fixJ [H]

Identifier: 237813035

GI number: 237813035

Start: 2725538

End: 2726176

Strand: Direct

Name: fixJ [H]

Synonym: GBP346_A2795

Alternate gene names: 237813035

Gene position: 2725538-2726176 (Clockwise)

Preceding gene: 237813034

Following gene: 237813036

Centisome position: 66.5

GC content: 64.32

Gene sequence:

>639_bases
ATGAATAGCCCTGTCACCACCTCACAGGAAACCGTCTTTGTCGTCGACGACGACGAGGCCGTGCGCGACTCGCTGCGCTG
GCTGCTGGAGGCAAACGGCTATCGCGTGCAATGCTTCTCGAGCGCCGAGCAGTTCCTCGATGCCTATCAGCCCGCGCAGC
AAGCGGGACAGATCGCGTGCCTGATCCTCGACGTGCGGATGTCGGGGATGAGCGGCCTCGAGCTGCAGGAGCGCCTGATC
GCCGAGAACGCGGCGCTCCCGATCATCTTCGTGACGGGCCACGGCGACGTGCCGATGGCTGTGTCGACGATGAAGAAAGG
CGCGATGGACTTCATCGAGAAGCCGTTCGACGAGGCCGAACTGCGCAAGCTCGTCGAGCGCATGCTCGAGAAGGCGCGAA
ACGAGAGCAAGAGCGTCCAGGAGCAGCGCGCGGCGAGCGAGCGGCTGTCGAAGCTGACGGCGCGCGAGCAGCAGGTGCTC
GAGCGGATCATCGCCGGGCGCCTGAACAAGCAGATCGCCGACGACCTCGGCATCAGCATCAAGACGGTCGAGGCGCACCG
CGCGAACATCATGGAAAAGCTCAACGTCAACACGGTCGCCGATCTGCTGCGCCTCGCGCTGTCGAAGAAGCAGGCGTAA

Upstream 100 bases:

>100_bases
TGTCGGGCGCGACGTTTCACTGCAGCCTGCCCATTGGGGAACCGGAGGATCTCGGTCGCGGATCCGAGACATCGCCATCA
CAAACCGTAACGGGAGAGAT

Downstream 100 bases:

>100_bases
CGCGCGGCACCCCGCTCTCCCTCTGCCGCCCGGCGCATGCCGGGCGAGCGCCGGTTTTTTGCGGCGCGCCGGGCGCGCGC
GACGTTCGCGGTTCCCGTCG

Product: transcriptional regulatory protein FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLEKARNESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA

Sequences:

>Translated_212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLEKARNESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA
>Mature_212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLEKARNESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=161, Percent_Identity=33.5403726708075, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=33.6734693877551, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=30.46875, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1786747, Length=153, Percent_Identity=26.797385620915, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=26.6129032258064, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=30.5970149253731, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23645; Mature: 23645

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIAC
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCEEE
LILDVRMSGMSGLELQERLIAENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAE
EEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRKLVERMLEKARNESKSVQEQRAASERLSKLTAREQQVLERIIAGRLNKQIADDLGISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSKKQA
EEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIAC
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCEEE
LILDVRMSGMSGLELQERLIAENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAE
EEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRKLVERMLEKARNESKSVQEQRAASERLSKLTAREQQVLERIIAGRLNKQIADDLGISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSKKQA
EEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]