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The map label for this gene is rluF [H]

Identifier: 237812812

GI number: 237812812

Start: 2498850

End: 2500565

Strand: Direct

Name: rluF [H]

Synonym: GBP346_A2566

Alternate gene names: 237812812

Gene position: 2498850-2500565 (Clockwise)

Preceding gene: 237812811

Following gene: 237812815

Centisome position: 60.97

GC content: 74.83

Gene sequence:

>1716_bases
ATGCGCACAAAATTGACCGTCAAGAATCCGCGCCCGGCGACGCCCGGCCGCGCCCCCGTCCGCTCCGGCAGCCTCACCGC
GCGCAAGGTCGCGCGGCCCGACCCGAAAGCGGCGGGCGCGAAACCCGCCGCGGCGAAGCCTGCTGCGAAGTCCGCATCGG
CTGCCAAGCCAGCGGCGCCGCGCGGCGCGGCGAACGCTGCGCCGAAGCGCGCGCCGGGGCCGTCGCACCCGGCCGCGGCA
TCCGAAGGCAAGCGCGTCGCGAAGCCGCGCACCGCGCACGACGCCGGCCGCACGGGCGGCGAGCGTGCGCCGGCCAAGCG
CGCCGCCGCGCCCGGTGCGCCCGGCGCGGCGTCCGCGCCGGGCACGCGCCGCACCGACGCGAAGCCGGCGCGCCGCACCG
ACGAACGCCCTGCCGGCCGCGCCGGCAATCGCCCTGCCGGCCGCGACGAGCGTGCACCGCGCGACTCGGATGCGCGCGCG
TTCGATGCGGGCACGCGCGGTAAGGACCGCGCGCCCCGCGAGGGCGCAAGGCCCGGCGCACGGGGCGCGACGGGCGCGAA
GTTCGGCGGCGCGGCGCACCGATCGGACGACGCCGACCGTCGAACGCCCCGCGCGACGCGTGCGGACAGCCGCGCGCGCG
ATGCCGCGCCGTCGTCGTTCGCGGGCAAGACCGCGACAGCCGGCAAGCGTGCGCCGCAGCGCGCCGACGATCGCTACGGC
GCAGCCGGGAAGCGCACATCGCCGCGCCCCGAGCGAACCGAGCGCCCCGCCCGCTTCGGCGAACGGCCGGCCACCCGCGC
GAGCGCATCCGGCGAGCGCCGCCCCACGGCCCGCGCGGCGACGGGTTCGCGCCTCAAGCTCGCGCAGCCGATCAAGCGCG
GCAGCGGCGAACTGGGCGAATCCGCTCGCGGCGGTGAGCACGGCGAACGCGGCAAGCGTATCGAGCGCGGCGACGAAACC
GGCCTCGTGCGCCTGTCGAAGCGCATGTCGGAGCTGGGTCTCTGCTCGCGCCGCGAAGCAGACGAATGGATCGAGAAAGG
CTGGGTGCTCGTCGACGGCGAGCGCATCGACACGCTCGGCACGAAGGTGCGCGCCGACCAGCGCATCGAGATCGATTCGA
ACGCGCGCGCCGCGCAGGCCGCGCAAGTGACGATCCTGCTGCACAAGCCGGTGGGCTACGTGTCGGGCCAGGCGGAGGAC
GGCTACGCCCCCGCCGCGACGCTCGTCACGCGCGAGAACCACTGGAGCGGCGACCGCTCGCCGCTGCGCTTCTCGCCGCA
GCACCTGCGCGCGCTCGCGCCCGCGGGCCGGCTCGACATCGATTCGACGGGCCTTCTCGTGCTGACGCAGAACGGGCGCG
TCGCGAAACAGCTGATCGGCGAACAATCGGACATCGACAAGGAATACCTGGTGCGCGTGCGCTTCGGCGAGCGCACGGCC
GACATCGAACGCCACTTCCCCGCCGAGTCGCTCGCGAAGCTGCGCCACGGCCTCGAGCTCGACGGCGTGCCGCTCAAGCC
CGCGATGGTCAGTTGGCAGAACGGCGAGCAACTGCGCTTCGTGCTGCGCGAAGGCAAGAAGCGCCAGATTCGCCGGATGT
GCGAACTCGTCGGCCTCGAGGTGATCGGCCTGAAGCGCGTGCGGATGGGCCGCGTGATGCTGGGCGCGCTGCCGCAAGGC
GAGTGGCGCTATCTCGGGCCGGACGAATCGTTCTGA

Upstream 100 bases:

>100_bases
TCTTCATGCTCGACGGCACCGGCTTGTCCGACAACCGTCTGCAGATCCAAGTCGAAACCGAGCTGCTGCGCGCGATCGCA
GTGTGAGTATTCCAGCGTTT

Downstream 100 bases:

>100_bases
GCACGGCGGCCGCCGTGCCCGGGCCGCGGCTGCGCGCGGGGCGGCGCAATAGCCGCGCGCCGCGCCGCCGCGGTTTGCGG
CAACGGGAATCGAACGAAAA

Product: RNA pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTKLTVKNPRPATPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKPAAKSASAAKPAAPRGAANAAPKRAPGPSHPAAA
SEGKRVAKPRTAHDAGRTGGERAPAKRAAAPGAPGAASAPGTRRTDAKPARRTDERPAGRAGNRPAGRDERAPRDSDARA
FDAGTRGKDRAPREGARPGARGATGAKFGGAAHRSDDADRRTPRATRADSRARDAAPSSFAGKTATAGKRAPQRADDRYG
AAGKRTSPRPERTERPARFGERPATRASASGERRPTARAATGSRLKLAQPIKRGSGELGESARGGEHGERGKRIERGDET
GLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAAQAAQVTILLHKPVGYVSGQAED
GYAPAATLVTRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTA
DIERHFPAESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQG
EWRYLGPDESF

Sequences:

>Translated_571_residues
MRTKLTVKNPRPATPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKPAAKSASAAKPAAPRGAANAAPKRAPGPSHPAAA
SEGKRVAKPRTAHDAGRTGGERAPAKRAAAPGAPGAASAPGTRRTDAKPARRTDERPAGRAGNRPAGRDERAPRDSDARA
FDAGTRGKDRAPREGARPGARGATGAKFGGAAHRSDDADRRTPRATRADSRARDAAPSSFAGKTATAGKRAPQRADDRYG
AAGKRTSPRPERTERPARFGERPATRASASGERRPTARAATGSRLKLAQPIKRGSGELGESARGGEHGERGKRIERGDET
GLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAAQAAQVTILLHKPVGYVSGQAED
GYAPAATLVTRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTA
DIERHFPAESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQG
EWRYLGPDESF
>Mature_571_residues
MRTKLTVKNPRPATPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKPAAKSASAAKPAAPRGAANAAPKRAPGPSHPAAA
SEGKRVAKPRTAHDAGRTGGERAPAKRAAAPGAPGAASAPGTRRTDAKPARRTDERPAGRAGNRPAGRDERAPRDSDARA
FDAGTRGKDRAPREGARPGARGATGAKFGGAAHRSDDADRRTPRATRADSRARDAAPSSFAGKTATAGKRAPQRADDRYG
AAGKRTSPRPERTERPARFGERPATRASASGERRPTARAATGSRLKLAQPIKRGSGELGESARGGEHGERGKRIERGDET
GLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAAQAAQVTILLHKPVGYVSGQAED
GYAPAATLVTRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTA
DIERHFPAESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQG
EWRYLGPDESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=35.7429718875502, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788510, Length=250, Percent_Identity=29.6, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=31.4741035856574, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 61082; Mature: 61082

Theoretical pI: Translated: 11.74; Mature: 11.74

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKLTVKNPRPATPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKPAAKSASAAKPAAP
CCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
RGAANAAPKRAPGPSHPAAASEGKRVAKPRTAHDAGRTGGERAPAKRAAAPGAPGAASAP
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCC
GTRRTDAKPARRTDERPAGRAGNRPAGRDERAPRDSDARAFDAGTRGKDRAPREGARPGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
RGATGAKFGGAAHRSDDADRRTPRATRADSRARDAAPSSFAGKTATAGKRAPQRADDRYG
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
AAGKRTSPRPERTERPARFGERPATRASASGERRPTARAATGSRLKLAQPIKRGSGELGE
CCCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCC
SARGGEHGERGKRIERGDETGLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLG
CCCCCCCCHHCCHHHCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHCCCEEECCCCHHHHH
TKVRADQRIEIDSNARAAQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENHWSGDRS
HHHCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTA
CCCCCHHHHHHHCCCCCCEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEEECCCHH
DIERHFPAESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLE
HHHHCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCH
VIGLKRVRMGRVMLGALPQGEWRYLGPDESF
HCCHHHHHHHHHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRTKLTVKNPRPATPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKPAAKSASAAKPAAP
CCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
RGAANAAPKRAPGPSHPAAASEGKRVAKPRTAHDAGRTGGERAPAKRAAAPGAPGAASAP
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCC
GTRRTDAKPARRTDERPAGRAGNRPAGRDERAPRDSDARAFDAGTRGKDRAPREGARPGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
RGATGAKFGGAAHRSDDADRRTPRATRADSRARDAAPSSFAGKTATAGKRAPQRADDRYG
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
AAGKRTSPRPERTERPARFGERPATRASASGERRPTARAATGSRLKLAQPIKRGSGELGE
CCCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCC
SARGGEHGERGKRIERGDETGLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLG
CCCCCCCCHHCCHHHCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHCCCEEECCCCHHHHH
TKVRADQRIEIDSNARAAQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENHWSGDRS
HHHCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTA
CCCCCHHHHHHHCCCCCCEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEEECCCHH
DIERHFPAESLAKLRHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLE
HHHHCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCH
VIGLKRVRMGRVMLGALPQGEWRYLGPDESF
HCCHHHHHHHHHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]