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The map label for this gene is lgrA [H]

Identifier: 237812468

GI number: 237812468

Start: 2178103

End: 2178954

Strand: Reverse

Name: lgrA [H]

Synonym: GBP346_A2217

Alternate gene names: 237812468

Gene position: 2178954-2178103 (Counterclockwise)

Preceding gene: 237812470

Following gene: 237812467

Centisome position: 53.16

GC content: 71.95

Gene sequence:

>852_bases
ATGCGACATCTGCTTACAACCGAAACGAAACGAAATCGCGGCGGCGCCAATCGCGGCGGCGTGCGGAAAGCGCCGCCGGG
CGCGCCTGCCGATGTGCGTCGCGCGCGGGCGGGGGCGCGCATCGACGCATCGACGCGTCGACGCTGCGGCTGCTGCGCGC
GGATCGCGATATTCGACGAGCGACTGCGCGAGGCGCAGAGCGGCGCCGTCGGCGAGATTGTCATCTCGGGCCCGTGTCTC
GCGCGCGGCTATCCCGACGATCCGAAGCAGGCCGCGGAGAAGTGCCTCGCGCATCCGTCGCGCGCGGGTGAGCGCGCCTA
TCGGATGGGCAACCTCGCGCGCCGGCTGCCCGACGGCGCGATCCAGTTCGTCGGCCGCCTCGACGGTCAGGTGAAGATCC
GCGGCTATCTCGTCGAGCCGGGCGAGGTCGAGATCGCGATCGCGCGGCAGCCGGGCGTGCGGCGGCGCGCCGTCGTCGCG
ACCTCGCCCGCCGACGGCGCGCCGAGAGAATTCGTCGCGTTCGCCGTGCCGGACGATCCGGCCGCGCCGCGCCGCGCCGC
CCGCTCGTCGGCCGCCTGCGCGCGGGCGTGGCCGCATCGCTGCCGCCTTTCATGGCGCACGGGCATTTCGCGATCATCGA
TGCGCTGCCGCTGTCCGCGAACGGCAAGACCGACAAGGCGGCGCTCGTCGCGATGCACGGGCCGTCCATCATTCGGCGCG
CGCGGTCAACTTCATCAAGCCGTATGCGGCGATGAAGCGCGACAACGTCGACGGGCTCGTCAACATGATCCGGTTCGCCG
CCGCCGCGCGCGTGAAGGCGCTGTCGCGGCTGTCGACGATCTCGGTCTATAG

Upstream 100 bases:

>100_bases
CGTCGGACGGACATCCGGAAGGCGGGGGCGGCGCGCCCGGGCGCTTGCACGCCGCGCTTTCCGCGCTATTAGTAGCACGG
CGGCGGGCCATTGGGCGGCA

Downstream 100 bases:

>100_bases
CTGGGGGCACCGGATCACGGGCAAGACCGTGATGCGCGAGGACGACGACCTCGACCAGAATCTCGACGCCGTGCGCGCCG
ACATCGGCTACGTGAAGAGC

Product: pristinamycin I synthase 3 and 4

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine/leucine adenylase; Val/LeuA; Valine/leucine activase; ATP-dependent glycine adenylase; GlyA; Glycine activase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRHLLTTETKRNRGGANRGGVRKAPPGAPADVRRARAGARIDASTRRRCGCCARIAIFDERLREAQSGAVGEIVISGPCL
ARGYPDDPKQAAEKCLAHPSRAGERAYRMGNLARRLPDGAIQFVGRLDGQVKIRGYLVEPGEVEIAIARQPGVRRRAVVA
TSPADGAPREFVAFAVPDDPAAPRRAARSSAACARAWPHRCRLSWRTGISRSSMRCRCPRTARPTRRRSSRCTGRPSFGA
RGQLHQAVCGDEARQRRRARQHDPVRRRRAREGAVAAVDDLGL

Sequences:

>Translated_283_residues
MRHLLTTETKRNRGGANRGGVRKAPPGAPADVRRARAGARIDASTRRRCGCCARIAIFDERLREAQSGAVGEIVISGPCL
ARGYPDDPKQAAEKCLAHPSRAGERAYRMGNLARRLPDGAIQFVGRLDGQVKIRGYLVEPGEVEIAIARQPGVRRRAVVA
TSPADGAPREFVAFAVPDDPAAPRRAARSSAACARAWPHRCRLSWRTGISRSSMRCRCPRTARPTRRRSSRCTGRPSFGA
RGQLHQAVCGDEARQRRRARQHDPVRRRRAREGAVAAVDDLGL
>Mature_283_residues
MRHLLTTETKRNRGGANRGGVRKAPPGAPADVRRARAGARIDASTRRRCGCCARIAIFDERLREAQSGAVGEIVISGPCL
ARGYPDDPKQAAEKCLAHPSRAGERAYRMGNLARRLPDGAIQFVGRLDGQVKIRGYLVEPGEVEIAIARQPGVRRRAVVA
TSPADGAPREFVAFAVPDDPAAPRRAARSSAACARAWPHRCRLSWRTGISRSSMRCRCPRTARPTRRRSSRCTGRPSFGA
RGQLHQAVCGDEARQRRRARQHDPVRRRRAREGAVAAVDDLGL

Specific function: Activates valine (or leucine, but much less frequently), and then glycine and catalyzes the formation of the peptide bond in the first step of peptide synthesis. This enzyme may also play a role in N-formylation of the first amino acid residue in the synt

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=99, Percent_Identity=37.3737373737374, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR002376
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00551 Formyl_trans_N; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 30972; Mature: 30972

Theoretical pI: Translated: 12.07; Mature: 12.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLLTTETKRNRGGANRGGVRKAPPGAPADVRRARAGARIDASTRRRCGCCARIAIFDE
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RLREAQSGAVGEIVISGPCLARGYPDDPKQAAEKCLAHPSRAGERAYRMGNLARRLPDGA
HHHHHHCCCCEEEEEECCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
IQFVGRLDGQVKIRGYLVEPGEVEIAIARQPGVRRRAVVATSPADGAPREFVAFAVPDDP
HHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCC
AAPRRAARSSAACARAWPHRCRLSWRTGISRSSMRCRCPRTARPTRRRSSRCTGRPSFGA
CCHHHHHHHHHHHHHHCHHHHEEHHHCCCCCCCCEECCCCCCCCHHHHHCCCCCCCCCCC
RGQLHQAVCGDEARQRRRARQHDPVRRRRAREGAVAAVDDLGL
CCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCEEEHHHCCC
>Mature Secondary Structure
MRHLLTTETKRNRGGANRGGVRKAPPGAPADVRRARAGARIDASTRRRCGCCARIAIFDE
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RLREAQSGAVGEIVISGPCLARGYPDDPKQAAEKCLAHPSRAGERAYRMGNLARRLPDGA
HHHHHHCCCCEEEEEECCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
IQFVGRLDGQVKIRGYLVEPGEVEIAIARQPGVRRRAVVATSPADGAPREFVAFAVPDDP
HHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCC
AAPRRAARSSAACARAWPHRCRLSWRTGISRSSMRCRCPRTARPTRRRSSRCTGRPSFGA
CCHHHHHHHHHHHHHHCHHHHEEHHHCCCCCCCCEECCCCCCCCHHHHHCCCCCCCCCCC
RGQLHQAVCGDEARQRRRARQHDPVRRRRAREGAVAAVDDLGL
CCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA