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The map label for this gene is lpfB [H]

Identifier: 237812411

GI number: 237812411

Start: 2133008

End: 2133853

Strand: Reverse

Name: lpfB [H]

Synonym: GBP346_A2159

Alternate gene names: 237812411

Gene position: 2133853-2133008 (Counterclockwise)

Preceding gene: 237812412

Following gene: 237812410

Centisome position: 52.06

GC content: 64.07

Gene sequence:

>846_bases
ATGCGTGCAAGTGAGGATGCAAAACCGGATTCCTTGACGCGGCCCCGAGCCGCGGCTTTCCCAGAGAGACGAAACATGCG
CCTGCCTACCGTTTGTCATGCCGCTTTTACCGCCGTCCTCGCCCTGTGCGGCACCGCAGCCCACGCGGGTATCCAGGTAG
GCGGCACACGCGTGGTTTTCGACGCGAAAGTCGATGTGCGCGATGCATCGGTTGCCGTGCGCAACAAGGGCGAGACGCCG
TACGTGATCCAGATTTTCGTCGACGACGGCAACGGCAATACCCGCCGCATGCCGTTTGCGGTGACGCCGCCGCTTTTTCG
GCTGGACGGCGGCAAGGAACAGCGCGTCAGCGTGCGTTACGTGAAGCGAGGCGCGGGGTTGCCGCAGGACGTCGAATCCG
TTTATTGGCTCAACGTCAAGGAGATTCCGCCCACCGAGAAGCGCAACGCGGACATCAACACGCTCAAGATCGCCGTCCTC
ACGCGCATCAAGCTCTTTTACCGGCCGGCGAGCCTTGCCGGCAGCGCGGCCGACGCGCCGTCGCGGCTCAAATGGTCCGT
CGTGCCGAACCCGATCGGGCAAGGCGTGGCGCTGAAGGTGAGCAACCCGAGCGCGTACCACGTGACCTTTTCGACGATCG
AGATTCAGGCCGCGCAGAACGCGAGCGTCAACGCCGACATGGTGAATCCGAAAGGCGAACTGATCGTTCCCATTCCGCCG
AAGGCCGTCTCGCAGGTCGGCCCCGTCAAATTCAGCTATACCACCATCAACGATTACGGCGCCGTCACGCCGGCGACGGA
AGTGACGGCCCAACCGGCCGGTACGCCCGAGGCCGCGCCGCAGTAG

Upstream 100 bases:

>100_bases
GCGCTATATCGCGACGTCCGCGACCGTGACGGGCGGCGCGGCCAATGCCACGGCGGTATTCGCGCTGACGTACAAGTAGC
GGGCGCCGAAGCGTTCCTGC

Downstream 100 bases:

>100_bases
GGCGCGATGATTCGGCATCGTGTCGCCGATGTCGGCTTCTCGCGCATACGAGTGACCATGCTGGCCGCCGCGCTCACGGC
GCTTTCGGCGACCGCTCGCG

Product: fimbrial assembly chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRASEDAKPDSLTRPRAAAFPERRNMRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETP
YVIQIFVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVL
TRIKLFYRPASLAGSAADAPSRLKWSVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPP
KAVSQVGPVKFSYTTINDYGAVTPATEVTAQPAGTPEAAPQ

Sequences:

>Translated_281_residues
MRASEDAKPDSLTRPRAAAFPERRNMRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETP
YVIQIFVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVL
TRIKLFYRPASLAGSAADAPSRLKWSVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPP
KAVSQVGPVKFSYTTINDYGAVTPATEVTAQPAGTPEAAPQ
>Mature_281_residues
MRASEDAKPDSLTRPRAAAFPERRNMRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVFDAKVDVRDASVAVRNKGETP
YVIQIFVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRYVKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVL
TRIKLFYRPASLAGSAADAPSRLKWSVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPP
KAVSQVGPVKFSYTTINDYGAVTPATEVTAQPAGTPEAAPQ

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1790771, Length=219, Percent_Identity=38.3561643835616, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786333, Length=234, Percent_Identity=32.4786324786325, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789532, Length=229, Percent_Identity=34.4978165938865, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786743, Length=233, Percent_Identity=32.618025751073, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787171, Length=238, Percent_Identity=33.1932773109244, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788428, Length=220, Percent_Identity=31.3636363636364, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788677, Length=212, Percent_Identity=33.0188679245283, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786936, Length=176, Percent_Identity=28.4090909090909, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082203, Length=230, Percent_Identity=24.3478260869565, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081806, Length=239, Percent_Identity=26.3598326359833, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 30096; Mature: 30096

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASEDAKPDSLTRPRAAAFPERRNMRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVF
CCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEECCEEEEE
DAKVDVRDASVAVRNKGETPYVIQIFVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRY
EEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCEECCEEECCCEEEECCCCCCEEEEEE
VKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVLTRIKLFYRPASLAGSAADAP
EECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEHEEECCHHHCCCCCCCC
SRLKWSVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPP
CCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCCCCCEEEECCCCCEEEECCC
KAVSQVGPVKFSYTTINDYGAVTPATEVTAQPAGTPEAAPQ
HHHHHCCCEEEEEEEECCCCCCCCCHHEEECCCCCCCCCCC
>Mature Secondary Structure
MRASEDAKPDSLTRPRAAAFPERRNMRLPTVCHAAFTAVLALCGTAAHAGIQVGGTRVVF
CCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEECCEEEEE
DAKVDVRDASVAVRNKGETPYVIQIFVDDGNGNTRRMPFAVTPPLFRLDGGKEQRVSVRY
EEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCEECCEEECCCEEEECCCCCCEEEEEE
VKRGAGLPQDVESVYWLNVKEIPPTEKRNADINTLKIAVLTRIKLFYRPASLAGSAADAP
EECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEHEEECCHHHCCCCCCCC
SRLKWSVVPNPIGQGVALKVSNPSAYHVTFSTIEIQAAQNASVNADMVNPKGELIVPIPP
CCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCCCCCEEEECCCCCEEEECCC
KAVSQVGPVKFSYTTINDYGAVTPATEVTAQPAGTPEAAPQ
HHHHHCCCEEEEEEEECCCCCCCCCHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]