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The map label for this gene is potH [H]

Identifier: 237812379

GI number: 237812379

Start: 2103814

End: 2104698

Strand: Reverse

Name: potH [H]

Synonym: GBP346_A2127

Alternate gene names: 237812379

Gene position: 2104698-2103814 (Counterclockwise)

Preceding gene: 237812380

Following gene: 237812378

Centisome position: 51.35

GC content: 63.84

Gene sequence:

>885_bases
ATGCCTAATGTACTGGGTTCCACCGCGCATCCGGCCGCGCGGCCGGCGCGTCGCCGACGAGATTGGCGCAGTTTTCGTCT
GCTCGCGCCGGCACTGCTGTTGCTCGTGATCTTCTTTCTGCTGCCCGTGCTGTCGCTGCTGCTGCACAGCATCCTCGAGC
CGACGCTGGGCTTGCAGAACTATACGCAACTGCTCGGTTCTACTACGTATTTGCGCGTGTTCGGTAACACTTTTCTCGTG
GCGACTGTCGTGACGGTCGCGACGGTCGCGATCGGCTTCCCGACGGCGTGGCTGCTTGCGATCGCGCCGCGCAAGGTGAG
CTCCCTGTTGTTTTCCATTTTGCTGCTGTCGATGTGGACGAACTTGCTGGCGCGGACGTTTGCCTGGATGGTACTCCTGC
AAGCGACGGGGCTGATCAACCGGACGCTGACGGCGCTGGGCCTCATTCATGAGCCGCTGGCACTCGTCAACAACCTCATC
GGCGTGACGATTGGCATGACCTACATCATGCTGCCGTTTCTGGTCATGCCGCTGCACGCGACGCTGCGCAGTATCGATCC
GTCGACGCTGCGGGCGGCTGCGGTCTGCGGTGCCAGCCGGTGGCAGGCGTTCTGGCGCGTGCTGGTGCCGCTGGCGATGC
CCGGGATCGCATCGGGTGCGCTGATGGTGTTCGTCATGGCGCTCGGCTACTTCGTGACCCCTGCATTGCTCGGCGGGCCT
TCGTACATGATGCTGGCCGAACTGATCGCGCAGCTCGTGCAGTCACTGCTGAACTGGGGACTCGCGGGTGCCGCCGCTTT
CGTGCTGCTGGCCGCGACGCTCTCGTTGTACGCGCTGCAACTCCGTTTCACCGGCAGTGCGCGCGCAAGCTTGGGAGGAC
GCTGA

Upstream 100 bases:

>100_bases
GTACGCCTGGCAATCGAAGTAAGCCGTTCGCCAGGCGAGGGGCTGTTGGCGGTTACAACGCCGATGGCCCCGTGCCGCTC
CGTCCGAATTGGGAGTCACC

Downstream 100 bases:

>100_bases
CATGCTTCTCGATTTCGACCGCTTGGGCGGGCTGCGCTGGGCGTTGATGGCGGTAGGCGGTGCCGTCGCTCTTTTCCTGC
TGCTGCCGATCGCGTTCATC

Product: ABC transporter, membrane spanning protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPNVLGSTAHPAARPARRRRDWRSFRLLAPALLLLVIFFLLPVLSLLLHSILEPTLGLQNYTQLLGSTTYLRVFGNTFLV
ATVVTVATVAIGFPTAWLLAIAPRKVSSLLFSILLLSMWTNLLARTFAWMVLLQATGLINRTLTALGLIHEPLALVNNLI
GVTIGMTYIMLPFLVMPLHATLRSIDPSTLRAAAVCGASRWQAFWRVLVPLAMPGIASGALMVFVMALGYFVTPALLGGP
SYMMLAELIAQLVQSLLNWGLAGAAAFVLLAATLSLYALQLRFTGSARASLGGR

Sequences:

>Translated_294_residues
MPNVLGSTAHPAARPARRRRDWRSFRLLAPALLLLVIFFLLPVLSLLLHSILEPTLGLQNYTQLLGSTTYLRVFGNTFLV
ATVVTVATVAIGFPTAWLLAIAPRKVSSLLFSILLLSMWTNLLARTFAWMVLLQATGLINRTLTALGLIHEPLALVNNLI
GVTIGMTYIMLPFLVMPLHATLRSIDPSTLRAAAVCGASRWQAFWRVLVPLAMPGIASGALMVFVMALGYFVTPALLGGP
SYMMLAELIAQLVQSLLNWGLAGAAAFVLLAATLSLYALQLRFTGSARASLGGR
>Mature_293_residues
PNVLGSTAHPAARPARRRRDWRSFRLLAPALLLLVIFFLLPVLSLLLHSILEPTLGLQNYTQLLGSTTYLRVFGNTFLVA
TVVTVATVAIGFPTAWLLAIAPRKVSSLLFSILLLSMWTNLLARTFAWMVLLQATGLINRTLTALGLIHEPLALVNNLIG
VTIGMTYIMLPFLVMPLHATLRSIDPSTLRAAAVCGASRWQAFWRVLVPLAMPGIASGALMVFVMALGYFVTPALLGGPS
YMMLAELIAQLVQSLLNWGLAGAAAFVLLAATLSLYALQLRFTGSARASLGGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=35.4545454545455, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787080, Length=216, Percent_Identity=35.6481481481481, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787713, Length=207, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31750; Mature: 31619

Theoretical pI: Translated: 12.10; Mature: 12.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVLGSTAHPAARPARRRRDWRSFRLLAPALLLLVIFFLLPVLSLLLHSILEPTLGLQN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YTQLLGSTTYLRVFGNTFLVATVVTVATVAIGFPTAWLLAIAPRKVSSLLFSILLLSMWT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLARTFAWMVLLQATGLINRTLTALGLIHEPLALVNNLIGVTIGMTYIMLPFLVMPLHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSIDPSTLRAAAVCGASRWQAFWRVLVPLAMPGIASGALMVFVMALGYFVTPALLGGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SYMMLAELIAQLVQSLLNWGLAGAAAFVLLAATLSLYALQLRFTGSARASLGGR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PNVLGSTAHPAARPARRRRDWRSFRLLAPALLLLVIFFLLPVLSLLLHSILEPTLGLQN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YTQLLGSTTYLRVFGNTFLVATVVTVATVAIGFPTAWLLAIAPRKVSSLLFSILLLSMWT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLARTFAWMVLLQATGLINRTLTALGLIHEPLALVNNLIGVTIGMTYIMLPFLVMPLHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSIDPSTLRAAAVCGASRWQAFWRVLVPLAMPGIASGALMVFVMALGYFVTPALLGGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SYMMLAELIAQLVQSLLNWGLAGAAAFVLLAATLSLYALQLRFTGSARASLGGR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]