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The map label for this gene is rluB [H]

Identifier: 237812039

GI number: 237812039

Start: 1727045

End: 1728709

Strand: Direct

Name: rluB [H]

Synonym: GBP346_A1781

Alternate gene names: 237812039

Gene position: 1727045-1728709 (Clockwise)

Preceding gene: 237812038

Following gene: 237812040

Centisome position: 42.14

GC content: 73.57

Gene sequence:

>1665_bases
TTGACTGATACCCACGACATCGATTCGTCCGAATCCGCGCATGCCGTTGCGACGGCGCGCGCCGACGACGCACCCGAGCA
GTCCGCAGCGGACGCGGGCGGCGAAGACCGCCCGCGCCGCGGTTTGCGGCGCGGGCCGCGCAGCCTGATCGCGCGCCGCC
GAGCGGCCGCGAAATCGAAGCATTCCGATGCGCCCGAAAGCGCCGACGCGGCGCCGGCGGCCGATGCCGGCGCGGGCGCC
GACGTCGCGAAAGCGCCCGCTCGCGCGCCGCGCGGCAAGGACGCCGCAGCGAAGCCGCCGCGCAAGACGGCGGGCAAGCG
CGAAGGCGCGGCGCGGCAGGGCGCTCAGCCGAAGCGAGGCGCGCAGCAGGCTGCCGCGGCGGTTGCGCCGTCCGCGGAGG
CCGGCCAGGACGACGTGTTCGCCTACGTGATTTCGCCGGCGTTCGACGCCGACAACAACGCGCCGGGCGGCGGCGTGCGC
GCGCCGATGCTGCGCCGGGGCCGCCAGACTCAGCCGAAGCGCGTGCTGTCGCCGGACGACGACGCGCCGAAGCTGCACAA
GGTGCTCGCGGAAGCCGGCATGGGCTCGCGCCGCGAGATGGAAGAGCTCATCATTGCCGGCCGGGTGTCGGTGAACGGCG
AGCCGGCGCACATCGGCCAACGGATCATGCCGACCGATCAGGTGCGGATCAACGGCAAGCCGGTCAAGCGCAAGCTGCCG
AGCAAGCCGCCGCGCGTGCTGCTGTATCACAAGCCGACGGGCGAGATCGTGAGCCACGCGGATCCGGAGGGCCGCCCGTC
CGTGTTCGATCGGCTGCCGCCGATGAAGACCGCGAAATGGCTCGCGGTCGGCCGCCTCGACTTCAACACCGAAGGCCTGC
TGATGCTGACGACGTCGGGCGATCTCGCGAACCGCTTCATGCATCCGCGCTATAGCGTCGAGCGCGAGTACGCGGTGCGC
GTCGTCGGCGAGCTGTCCGAGGCGTCGCGTCAGAAGCTGCTGCACGGCGTCGAGCTCGACGACGGCCCGGCGAATTTCCT
GCGCATTCGCGACGGCGGCGGCGAAGGCACGAATCACTGGTATCACGTCGCGCTTGCCGAAGGGCGCAACCGCGAGGTGC
GGCGGATGTTCGAGGCGGTCGGCCTGATGGTGAGCCGCCTGATCCGCACGCGCCACGGCCCGATCCCGCTGCCGCGCGGG
TTGAAGCGCGGCCGCTGGGAGGAACTCGACGAGGCGCAGGTGCGGCGCCTGATGTCGACGGTCGGCCTGAAGGCGCCGAC
CGAGGATAAGGGCGGCAAGCGCGGCGGCCCGGCCGAGCGCCGCCAGCCCGATCCGATGCAGACGTCGATGGGCTTCATCA
ATCGCGAGCCCGTGCTGACGACTCACGGCCAGCTCGACCAGCCGCGGCGCGGCCGCCGCGGGCCGGCGGGCGGCGGCTTC
GGCGCGGGCCTCGGCGGCGGCTACGCCGGCCTGCCGGGCTACGGCGGCGCGTCGCGCCAGGGCGGCCGCGATGTCGACGG
CAACCGCGCGTCCTACGGCGGCGCGGGCGCGAACAAGCGCGGCGCCGGCAAGGGCGGCCGCAATCCGAACGGCAATCGCG
CCGAAGGCGGTGCGCGCGGCGGCCCGCGTACGCCGCAGCAGCGCAATCGTTCGCGTAGCCGCTGA

Upstream 100 bases:

>100_bases
GGACGACGAGGACGCGCCTGCGAAGCGTCGCGCCTGAGACGGGCGGCCGAACCCCAGCCGTGCCCGCGACGGGCGCGAGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
TTGCGCGAGAACGGCATTCGCAACCGCCGCGCGCGTTGCGAATGCCTCGCAAAGTCGCCGTTTCGGGCTGATTTTGCGGG
GCCGAGCTTTGCGTTCGGGC

Product: RNA pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTDTHDIDSSESAHAVATARADDAPEQSAADAGGEDRPRRGLRRGPRSLIARRRAAAKSKHSDAPESADAAPAADAGAGA
DVAKAPARAPRGKDAAAKPPRKTAGKREGAARQGAQPKRGAQQAAAAVAPSAEAGQDDVFAYVISPAFDADNNAPGGGVR
APMLRRGRQTQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLP
SKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVR
VVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRG
LKRGRWEELDEAQVRRLMSTVGLKAPTEDKGGKRGGPAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGRRGPAGGGF
GAGLGGGYAGLPGYGGASRQGGRDVDGNRASYGGAGANKRGAGKGGRNPNGNRAEGGARGGPRTPQQRNRSRSR

Sequences:

>Translated_554_residues
MTDTHDIDSSESAHAVATARADDAPEQSAADAGGEDRPRRGLRRGPRSLIARRRAAAKSKHSDAPESADAAPAADAGAGA
DVAKAPARAPRGKDAAAKPPRKTAGKREGAARQGAQPKRGAQQAAAAVAPSAEAGQDDVFAYVISPAFDADNNAPGGGVR
APMLRRGRQTQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLP
SKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVR
VVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRG
LKRGRWEELDEAQVRRLMSTVGLKAPTEDKGGKRGGPAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGRRGPAGGGF
GAGLGGGYAGLPGYGGASRQGGRDVDGNRASYGGAGANKRGAGKGGRNPNGNRAEGGARGGPRTPQQRNRSRSR
>Mature_553_residues
TDTHDIDSSESAHAVATARADDAPEQSAADAGGEDRPRRGLRRGPRSLIARRRAAAKSKHSDAPESADAAPAADAGAGAD
VAKAPARAPRGKDAAAKPPRKTAGKREGAARQGAQPKRGAQQAAAAVAPSAEAGQDDVFAYVISPAFDADNNAPGGGVRA
PMLRRGRQTQPKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLPS
KPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRV
VGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGL
KRGRWEELDEAQVRRLMSTVGLKAPTEDKGGKRGGPAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGRRGPAGGGFG
AGLGGGYAGLPGYGGASRQGGRDVDGNRASYGGAGANKRGAGKGGRNPNGNRAEGGARGGPRTPQQRNRSRSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=252, Percent_Identity=56.7460317460317, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=29.9180327868852, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788510, Length=214, Percent_Identity=30.3738317757009, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.6787564766839, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 59042; Mature: 58911

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTHDIDSSESAHAVATARADDAPEQSAADAGGEDRPRRGLRRGPRSLIARRRAAAKSK
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHC
HSDAPESADAAPAADAGAGADVAKAPARAPRGKDAAAKPPRKTAGKREGAARQGAQPKRG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCCHHH
AQQAAAAVAPSAEAGQDDVFAYVISPAFDADNNAPGGGVRAPMLRRGRQTQPKRVLSPDD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLP
CCHHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEECCCHHHHCCC
SKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSG
CCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEECCC
DLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCE
YHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRRLMST
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VGLKAPTEDKGGKRGGPAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGRRGPAGGGF
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
GAGLGGGYAGLPGYGGASRQGGRDVDGNRASYGGAGANKRGAGKGGRNPNGNRAEGGARG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPRTPQQRNRSRSR
CCCCCHHHHHCCCC
>Mature Secondary Structure 
TDTHDIDSSESAHAVATARADDAPEQSAADAGGEDRPRRGLRRGPRSLIARRRAAAKSK
CCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHC
HSDAPESADAAPAADAGAGADVAKAPARAPRGKDAAAKPPRKTAGKREGAARQGAQPKRG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCCHHH
AQQAAAAVAPSAEAGQDDVFAYVISPAFDADNNAPGGGVRAPMLRRGRQTQPKRVLSPDD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DAPKLHKVLAEAGMGSRREMEELIIAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKLP
CCHHHHHHHHHHCCCCHHHHHHHHHHCEEECCCCHHHHCCCCCCCCEEEECCCHHHHCCC
SKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSG
CCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEECCC
DLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCE
YHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRRLMST
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VGLKAPTEDKGGKRGGPAERRQPDPMQTSMGFINREPVLTTHGQLDQPRRGRRGPAGGGF
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
GAGLGGGYAGLPGYGGASRQGGRDVDGNRASYGGAGANKRGAGKGGRNPNGNRAEGGARG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPRTPQQRNRSRSR
CCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]