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The map label for this gene is iolD [H]

Identifier: 237811959

GI number: 237811959

Start: 1639173

End: 1641158

Strand: Reverse

Name: iolD [H]

Synonym: GBP346_A1699

Alternate gene names: 237811959

Gene position: 1641158-1639173 (Counterclockwise)

Preceding gene: 237811960

Following gene: 237811958

Centisome position: 40.04

GC content: 73.01

Gene sequence:

>1986_bases
ATGAATCGCAGAGCAATGGATGACGACACCCGACGGAGCCCGAGCGTGAACGACTTCATCGACGATCCGGCCGCGAGCGT
GCAACGCACGGTCCGGCTGACCGCCGCGCAGGCGCTCGTGCGCTATCTGGCCGCGCAGCGCGTCGCGACCGGCGACGGCG
ACGCGCCGCTGTTCGGCGGCGTGTTCGCGATCTTCGGGCACGGCAACGTCGCGGGCCTGGGCGAGGCGCTGCATCAATAC
CGCGACGCGCTGCCGACCTACCGCGCGCACAACGAGCAGGCGATGGCGCACAGCGCGATCGCGTACGCGAAGGCGCACCT
GCGCCGCCGGATGATGGCCGTGACGACATCGATCGGCCCCGGCGCGACGAACCTCGTCACCGCGGCCGCGCTCGCGCACG
TGAACCGGCTGCCGGTGCTGCTGCTGCCGGGCGACGTGTTCGTGTCGCGCGCGCCGGACCCGGTGCTGCAGCAGATCGAG
GATTTCCACGACGGCGCGATCTCGGCGAACGACGCGTTCAAGGCCGTGTCGCGCTACTTCGACCGGATCGTGCATCCGGC
CCAGTTGCTCACCGCGCTGCCGCGCGCGCTGCGCGTGCTGACCGACGCCGCGCTCTGCGGCCCCGTGACGCTCGCGCTGC
CGCAGGACGTGCAGACGATGGCCTACGACTACCCGGCCGAGTTCTTCGCGCCGCGCGCCGTCACGTTCCATGCGCCCGCG
CCCGTCGAGGCGGAGCTCGACGCGGCGCTCGCGCCACTGCGCGCGTCGGCCCGGCCGTTCATCGTCGCGGGCGGCGGCGC
GCTATACAGCGAAGGCGGCGCGGCCGCGCTCGCCGCGTTCGCCGAGCGCCACGGCATCCCGGTCGGCGAGACGCAGGCCG
GCAAGAGCGCACTGCCGTGGGACCACCCGCTGAACGCCGGCGCGATCGGCGTGACGGGCTCGCCCGCGGCGAACGCGCTC
GCGCACGATGCCGATTGCGTGATCGCGATCGGCACGCGGCTGCAGGATTTCACGACCGGCTCGAACACGCTGTTCGCGCG
CGCACGCGTCATCGGCATCAACGCGAACGCGTTCGATGCGCTCAAGCATCGCGCGACGATCGTGCAGGCGGACGCGCGGC
TCGCGCTCGACGCGCTCGGCGCGCGCCTCGAAGGCTGGCGCGCCGACGCCGCGTGGACGGCGCGCGCGCACGCGCGCGCG
AACGCATGGCGCGACGCCGTCGCGGCCGTCACGCATGCGCCGCCGCGCGCGCGGGCGCTGCCGTACGACGCCGACGTGAT
CGGCGCCGTGCAGCGCTCGGCGCCCGATTCGGCCGCGCACGACATCGCCGTGTGCGCGGCGGGCACGCTGCCCGCCGAGC
TGCACAAGCTGTGGCGCGCGGCGGCGCCGGGCGGCTATCACGTCGAATACGGCTATTCGTGCATGGGCTACGAGATCGCG
GGCGGGCTCGGCGCGAAGCTCGCGAAGCCCGAGCGCGAGGTGATCGTGATGGTCGGCGACGGCAGCTATCTGATGATGAA
CAGCGAGATCGCGACGTCGGTGATGCTCGGCGCGAAGCTGATCGTCGTGGTGCTCGACAACCGCGGCTACGGCTGCATCA
ACCGGCTGCAGCAGGCGTGCGGCGGCGCGCCGTTCAACAACCTGTTCGACGACTGCGTGCAAGGCCCGCTCGGCGCGCCG
CGCATCGATTTCGCCGCGCACGCGCGCGCGCTCGGCGCGCGCGCGGAGCACGTCGCCGATATCGCCGCGCTCGAAGCGGC
GATGGCGCGCGCCCGCGCGTCCGATCGCACGTACGTGATCTGCATCGACACCGATCCCGCCCGCACGACGCCCGACGGCG
GCTGGTGGTGGGAAGTCGCGGTGCCCGAAGTGTCGCCGCGCGACGCGGTGCGCGACGCGCGCGCGGATTACGAACGGCAC
CTCGCCGCGCGCGCAGGCGCTCCCGCCTCTCGTTCAACCCCCTCTACTCCGGAATCATCGACATGA

Upstream 100 bases:

>100_bases
GAGACGCTGATCGGCGCGTGGCGCGCGGCCCGCACGGACGCCCGCGCGCGCGGCCGCCACGCCGGCGCCGCGCCGCGCGT
GGCCGCCTAGGAGCCGCGCG

Downstream 100 bases:

>100_bases
GCGCACCCGACATCCGTATCGGCATCAACCCGCTGTCGTGGATGAACGACGATCTGCCGTCGCTCGGCGGCGAGACGCCG
CTCGCCGTCGCGCTGACCGA

Product: IolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MNRRAMDDDTRRSPSVNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQY
RDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIE
DFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPA
PVEAELDAALAPLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANAL
AHDADCVIAIGTRLQDFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARA
NAWRDAVAAVTHAPPRARALPYDADVIGAVQRSAPDSAAHDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIA
GGLGAKLAKPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAP
RIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERH
LAARAGAPASRSTPSTPESST

Sequences:

>Translated_661_residues
MNRRAMDDDTRRSPSVNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQY
RDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIE
DFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPA
PVEAELDAALAPLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANAL
AHDADCVIAIGTRLQDFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARA
NAWRDAVAAVTHAPPRARALPYDADVIGAVQRSAPDSAAHDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIA
GGLGAKLAKPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAP
RIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERH
LAARAGAPASRSTPSTPESST
>Mature_661_residues
MNRRAMDDDTRRSPSVNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQY
RDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIE
DFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPA
PVEAELDAALAPLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANAL
AHDADCVIAIGTRLQDFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARA
NAWRDAVAAVTHAPPRARALPYDADVIGAVQRSAPDSAAHDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIA
GGLGAKLAKPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAP
RIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERH
LAARAGAPASRSTPSTPESST

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=530, Percent_Identity=25.6603773584906, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790104, Length=525, Percent_Identity=25.5238095238095, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=26.2172284644195, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787096, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=537, Percent_Identity=24.0223463687151, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531299, Length=538, Percent_Identity=24.7211895910781, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=607, Percent_Identity=23.0642504118616, Blast_Score=126, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69656; Mature: 69656

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRAMDDDTRRSPSVNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGG
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
VFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGP
EEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKAVSRYF
CHHHHHHHHHHHHHCCCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
DRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHCCCCEEEEECCC
PVEAELDAALAPLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPW
CCCHHHHHHHHHHHCCCCCEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
DHPLNAGAIGVTGSPAANALAHDADCVIAIGTRLQDFTTGSNTLFARARVIGINANAFDA
CCCCCCCEEECCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHHH
LKHRATIVQADARLALDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPPRARAL
HHHHHHEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYDADVIGAVQRSAPDSAAHDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIA
CCCHHHHHHHHHCCCCCHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCEEECEEEC
GGLGAKLAKPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQAC
CCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
GGAPFNNLFDDCVQGPLGAPRIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVI
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPSTPESS
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
T
C
>Mature Secondary Structure
MNRRAMDDDTRRSPSVNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGG
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC
VFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGP
EEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKAVSRYF
CHHHHHHHHHHHHHCCCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
DRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHCCCCEEEEECCC
PVEAELDAALAPLRASARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPW
CCCHHHHHHHHHHHCCCCCEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
DHPLNAGAIGVTGSPAANALAHDADCVIAIGTRLQDFTTGSNTLFARARVIGINANAFDA
CCCCCCCEEECCCCCHHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHHH
LKHRATIVQADARLALDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPPRARAL
HHHHHHEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYDADVIGAVQRSAPDSAAHDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIA
CCCHHHHHHHHHCCCCCHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCEEECEEEC
GGLGAKLAKPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQAC
CCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
GGAPFNNLFDDCVQGPLGAPRIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVI
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPSTPESS
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA